Compositions and methods for agrobacterium mediated transformation of chloroplasts in seed plants

ABSTRACT

Compositions and methods for agrobacterium-mediated chloroplast transgene expression are provided.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims priority to U.S. Provisional Application No. 63/223,881, filed on Jul. 20, 2021, the entire disclosure of which is incorporated herein by reference as though set forth in full.

INCORPORATION BY REFERENCE OF MATERIAL SUBMITTED IN ELECTRONIC FORM

The contents of the electronic sequence listing (RUT-102-US.xml; Size: 294,637 bytes; and Date of Creation: Jul. 20, 2022) is herein incorporated by reference in its entirety.

GRANT STATEMENT

This invention was made with government support under grant numbers MCB 1716102 and IOS 2037155 awarded by the National Science Foundation. The government has certain rights in the invention.

FIELD OF THE INVENTION

The invention relates to fields of transgenic plants and efficient means for expression of heterologous proteins and RNAs of interest. More specifically, the invention provides methods and compositions to increase the efficiency of plant cell transformation and to engineer desirable plant traits through Agrobacterium-mediated delivery of specific proteins and RNAs into targeted organelles (e.g., plastids, such as chloroplasts) in plants of interest.

BACKGROUND OF THE INVENTION

Several publications and patent documents are cited throughout the specification in order to describe the state of the art to which this invention pertains. Each of these citations is incorporated herein by reference as though set forth in full.

Agrobacterium tumefaciens is a Gram-negative phytopathogenic bacterium considered a natural genetic engineer because it transforms plants and results in crown gall disease. The gall is caused by the transferred DNA (T-DNA) encoding genes for plant growth regulators, auxins and cytokinins, that trigger cell division. The T-DNA also encodes genes for the synthesis of the opines: octopine (ocs), mannopine (mas) and agropine (ags). Opines are metabolites only Agrobacterium can utilize, thus the objective of T-DNA transfer is towards creating an exclusive food source for the species. The T-DNA is defined by 25-bp direct repeats which form the T-DNA borders. The T-DNA and genes required for T-DNA transfer (vir genes) are encoded on mega-plasmids, such as pTiC58 in A. tumefaciens strain C58 (Wood et al., 2001, Gelvin, 2003).

The T-DNA transfer process is triggered by plant metabolites, such as sugars and phenolic compounds via a two-component sensory response system consisting of constitutively expressed bacterial VirA and VirG proteins. VirA detects plant signaling molecules and phosphorylates VirG, which activates transcription of other vir genes on the Ti plasmid. VirD2, an endonuclease, in concert with VirD1, nicks the left and right border sequences of the T-DNA, and VirD2 covalently attaches to the 5′ end of single-stranded T-DNA (T-strand). The VirD2/T-strand complex along with other effector proteins including VirE2, VirE3, VirF and VirD5 are translocated to the plant cell through a Type IV secretion system (T4SS) composed of VirB 1-11 and VirD4 (Gelvin, 2003, McCullen and Binns, 2006).

In the plant cytoplasm, VirE2 covers the T-strand of the VirD2/T-strand complex, forming the T-complex to protect it from degradation. The T-complex is directed to the nucleus by VirD2, which has nuclear localization signals. VirE2 also participates in the nuclear targeting of the complex and facilitates the movement of the T-complex through the nuclear pore. Plant proteins such as importin α, β, CAK2Ms kinase, protein phosphatase 2C and VirE2 interacting protein 1 (VIP1) form complexes with either VirD2 and/or VirE2 for nuclear targeting. Upon entry of the complex in the nucleus, VirF recognizes VIP1 which leads to the destabilization and degradation of VirE2 and VIP1 via the Skp-Cullin-F-box pathway. Subsequently, the T-strand integrates into the nuclear genome by a variant of double-strand break repair (Brunaud et al., 2002, Tzfira et al., 2004, Gelvin, 2017).

Arabidopsis (Arabidopsis thaliana) is the best characterized model plant and is used to study all aspects of basic science. A notable exception is that studies involving plastid genome engineering are also carried out in tobacco, the only vascular plant species in which plastome engineering is routine. Recently, high-frequency plastid transformation in Arabidopsis was achieved by using plants hyper-sensitive to spectinomycin, the selective agent used in chloroplast transformation. The current bottleneck of plastid transformation in Arabidopsis is the difficulty of obtaining fertile plants from transplastomic tissue culture cells. It is an objective of the invention to create an Agrobacterium based system for efficient transformation of chloroplasts in seed plants which avoids the need for tissue culture-based selection.

SUMMARY OF THE INVENTION

Disarmed strains of Agrobacterium have been developed providing an efficient and practical gene transfer tool. By removing both the disease-causing and opine genes, the virulence plasmid is transformed to selectively aid the excision of T-DNA containing genes of interest from a modified binary plasmid. In one aspect, the invention provides a method for delivering a nucleic acid encoding a polypeptide or RNA of interest to plant cell chloroplasts. An exemplary method comprises providing an Agrobacterium variant strain lacking VirD2 in the Vir operon, introducing a binary vector plasmid encoding a polynucleotide fusion construct encoding a 1) VirD2 variant protein and chloroplast transit peptide operably linked to a promoter and terminator which drives expression in said Agrobacterium variant strain, and 2) a T DNA comprising a chloroplast sequence mediating homologous recombination into the chloroplast genome, a nucleic acid encoding a polypeptide or RNA of interest, and a nucleic acid encoding a selectable marker, wherein said VirD2 variant protein forms a complex with said T-DNA and delivers said complex to the chloroplast. Infected plant cells are then identified as those which survive in the presence of a selection agent, thereby identifying cells harboring the T-DNA and expressing said polynucleotide of interest. In certain embodiments the VirD2 variant protein expressed from the binary plasmid comprises a sequence selected from SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 42 and SEQ ID NO: 43. The selectable marker in the binary vector can be encoded by a sequence selected from SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO 40, SEQ ID NO: 41 and SEQ ID NO: 55.

The polynucleotide fusion construct can be present in a binary vector backbone selected from a pAGa vector of SEQ ID NO: 22, a pAGb vector of SEQ ID NO: 23, a pAGc vector of SEQ ID NO: 24, and a pAGd vector of SEQ ID NO: 25.

In a preferred embodiment, the pAG binary vector encoding said PtVirD2 variant is introduced into a XYA105ΔvirD2 strain for co-cultivation and chloroplast transformation in a plant. In yet another aspect, the pAG binary vector comprises a PtVirD2 variant encoded by SEQ ID NO: 29.

The invention also provides an isolated Agrobacterium strain XYA105Pt variant selected from XYA105Pt45, XYA105Pt46, and XYA105Pt47, comprising a reconstituted virD operon encoding a VirD2 variant selected from SEQ ID NO: 45, SEQ ID NO: 46 and SEQ ID NO: 47, said variant Agrobacterium strain optionally comprising a binary vector harboring a T-DNA encoding a nucleic acid of interest and a selectable marker, said T-DNA comprising a sequence which mediates homologous recombination in a chloroplast genome in said plant cell. In another aspect, the plant is infected with a XYA105ΔvirD2 Agrobacterium strain carrying said binary vector comprising Pt-VirD2 variant and said T-DNA.

In certain embodiments, the plant cell is a tobacco, Arabidopsis, corn, soybean, sorghum, wheat, rice, alfalfa, sunflower, or Brassica sp. cell. In certain embodiments, the selectable marker protein can confer a visibly selectable phenotype or it can confer resistance to a selection agent.

In a preferred embodiment, the polynucleotide fusion construct further comprises an operably linked polynucleotide encoding a transit polypeptide sequence, wherein said transit polypeptide sequence targets said VirD2 fusion polypeptide to a subcellular organelle other than the nucleus. In a particularly preferred embodiment, the subcellular organelle is a chloroplast.

The Agrobacterium can be a mutant Agrobacterium tumefaciens strain comprising a mutation in one or more of a virA, virG, virB, virD or virE gene. In certain embodiments, the plant cell is a monocot. In other embodiments, the plant cell is a dicot. The method described above can further include the step of growing the plant cell to produce a plant. In an alternative embodiment, the method can further comprise exposing a flower from the plant to the Agrobacterium, harvesting seed from said flower and optionally germinating said seed on selective media. In another aspect, the method further comprises exposing a flower from the plant to the Agrobacterium, harvesting seed from said flower and identifying transplastomic events by leaf phenotypic change.

In another aspect of the invention, a method for delivering a polynucleotide of interest to plant cell chloroplasts is disclosed. In this embodiment, the method comprises providing an Agrobacterium variant strain with a wild type Vir operon, introducing a binary vector plasmid encoding 1) a polynucleotide fusion construct encoding a VirD2 variant protein operably linked to a promoter and terminator and a chloroplast transit peptide which drives expression in said Agrobacterium variant strain, and 2) a T DNA comprising a chloroplast sequence mediating homologous recombination into the chloroplast genome, a nucleic acid encoding a polypeptide of interest, and a nucleic acid encoding a selectable marker, wherein said VirD2 variant protein forms a complex with said T-DNA and delivers said complex to the chloroplast, and selecting infected plant cells which survive in the presence of a selection agent, thereby identifying cells expressing said polynucleotide of interest. As above, the VirD2 variant protein can be expressed from a binary plasmid comprising a sequence selected from SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 42 and SEQ ID NO: 43.

Also provided is a kit for practicing the methods of any of the preceding method claims.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 . VirD2 and Pt-VirD2 protein domains. VirD2 contains a relaxase (endonuclease) domain, a domain of unknown function (DUF), a N-terminal monopartite type NLS (NLS1), a C-terminal bipartite type NLS2 and Y29, NLS 3 in in DUF region. Y29 is tyrosine 29, to which VirD2 links T-DNA. In the Pt-VirD2 protein TP is the Rubisco small subunit transit peptide, 5 aa are the five N-terminal amino acids of the Rubisco small subunit, R32T is the arginine to threonine mutation inactivating NLS 1.

FIGS. 2A - 2B. Schematic diagram of bar^(au) gene excision by Agro-Int or Nuclear-Int yielding plastid genome Nt-pSS33Δ. FIG. 2A) Agro-Int is expressed in Agrobacterium from plasmid pJCD6B and exported to chloroplasts. The tobacco Rubisco small subunit transit peptide (TP) and the VirF (F) T4SS signal were fused at the Int N- and C-termini, respectively. PvE, Promoter virE; TvF, terminator virF. FIG. 2B) Co-cultivation of Agrobacterium carrying a binary plastid pKO117 (Lutz et al., 2004) is followed by the transfer of the T-complex to the nucleus, symbolized with a blue circle (VirD2) and a black line (T-strand). Int is expressed in a nuclear P2′/Tnos cassette and is fused with the pea Rubisco small subunit transit peptide for plastid targeting. T1-ptDNA and T2-ptDNA refer to transformed plastid genomes before and after bar^(au) excision.

FIGS. 3A -3E. Integrase exported from Agrobacterium excises bar^(au) gene in the Nt-pSS33 chloroplast genome. FIG. 3A) Partial map of the wild-type plastid genome where arrow marks the site of transgene insertion. FIG. 3B) Partial map of the Nt-pSS33 plastid genome before and after the excision of bar^(au) gene. Shown are: the trnI, rrn16 and trnV plastid genes and of the transcription start site of 3’rps12/7 operon promoter; the attB, attP target sites and the attR recombination junction; the AM102 and AM103 primer sites; and the BamHI, EcoRI, and ApaI restriction sites. FIG. 3C), The minimal attB/attP sequence [SEQ ID NO 57, SEQ ID NO 58]: and the attL and attR recombination junctions (SEQ ID NO: 59, SEQ ID NO 60) (Groth et al., 2000). FIG. 3D) Tobacco leaf three days after Agroinfiltration. FIG. 3E) PCR amplification confirms target excision in syringe injected leaves. The AM102 and AM103 primers amplify a 1.9 kb fragment before, and a 0.9 kb fragment after excision of the bar^(au) gene.

FIG. 4 . Schematic of the split GFP system to detect Pt-VirD2 fluorescence in plant chloroplasts.

FIG. 5 . Plasmids for the split-GFP assay in E. coli. pAM305 is a chloroplast transformation vector carrying GFP1-10 and aadA genes under the chloroplast regulatory elements flanking with chloroplast homologous sequences. pAM31-36 vectors encode different configurations of Pt-VirD2 (TP:: virD2MRwt::F), GFP11, placed at either N- or C- terminus with or without the linker [L, (GGGS)x2; L*, GGGS], and the T4SS signal of VirF (F). pAM30 does not contain GFP11 and used as a complementation negative control. In pAM30-36, the genes can be induced by IPTG to determine the most efficient configuration.

FIG. 6 . Fluorescence in E. coli confirms complementation of GFP1-10 and VirD2-11 proteins. Transformants were selected in E. coli on LB plates containing 100 mg/L spectinomycin and 50 mg/L kanamycin. Plates contained 1 mM IPTG. The positive control was plasmid pAM29 [SEQ ID NO 6], expressing sulfite-reductase-GFP11. The negative control was pAM30 [SEQ ID NO 7], Pt-VirD2 without GFP 11.

FIG. 7 . Split-GFP assay in chloroplasts after split GFP expression from nuclear genes and transport to the chloroplasts.

FIG. 8 . Plasmids for the split-GFP assay in chloroplasts for expression from nuclear genes. pAM407 binary vector encodes TP-GFP1-10 gene and kanamycin resistance gene. pJF7-12 binary vectors carry different configurations of Pt-VirD2 (TP:: virD2MRwt::F), GFP11, placed at either N- or C- terminus with or without the linker [L, (GGGS)x2; L*, GGGS], and the T4SS signal of VirF (F). Pt-virD2-11 genes expressed in an Arabidopsis ubiquitin promoter/terminator cassette (PAtUBQ/TAtUBQ).

FIG. 9 . The T-DNA region of pAG binary vectors. Shown are the kanamycin resistance bacterial marker gene in the backbone, and the left border (LB) and right border (RB) repeats. Note duplicated 25 bp LB repeats, and the RB with the 24 nt overdrive (OD, gray box). To facilitate cloning of sequences for T-DNA transfer, we included the PacI, I-SecI and AscI restriction sites between the LB and RB. The a, b, c, and d derivatives differ in the position of multiple cloning sites (MCS).

FIG. 10 . The T-DNA region of pAG-CpTEST (also referred to as Pwise in FIG. 13A) binary chloroplast transformation vector. The chloroplast targeting regions contains nucleotides 104,087-101,147 of the tobacco ptDNA (NC_001879.2) with the dicistronic aadA-gfp gene as marker. We show above the MCS a Pt-VirD2 gene that could be any gene listed in Table 1..

FIG. 11 . The pAG-CHL-1 Agrobacterium binary vector for chloroplast transformation. The vector carries the aadA gene from vector pMRR13, an MCS and a Pt-VirD2gene. [SEQ ID NO 29). The Pt-VirtD2 gene can be any of the variants listed as SEQ ID NO 27, SEQ ID NO 28, SEQ ID NO 31, SEQ ID NO 32, SEQ ID NO 42, SEQ ID NO 43.

FIG. 12 . Reconstituting the virD operon with Pt-virD2 replacing wild type virD2 gene in the operon. On top is sacB plasmid with kanamycin resistance as bacterial marker and part of the virD1-Pt-virD2-virD3 operon for insertion in the virulence plasmid. Cointegrate formation via the D1 (left) or D3 (right) genes is shown, followed by loss of duplicated structures via second homologous recombination event yielding the desired reconstituted operon, or restoration of the original virD2-lacking structure.

FIGS. 13A-13D. Agrobacterium strains for Agrobacterium-mediated chloroplast transformation. FIG. 13A) pWISE binary vector for Agrobacterium-mediated chloroplast transformation. pWISE vector carries the chloroplast transformation vector flanked by T-DNA borders and a plastid-targeted Pt-virD2 gene variant. The Pt-virD2 gene can be any gene listed in Table 1. The pXYA105ΔvirD2 virulence plasmid lacks virD2 gene in the virD operon. FIG. 13B) virD operon in pXYA105ΔvirD2 Agrobacterium strain. FIG. 13C) pWISE0 binary vector in XYA105Pt Agrobacterium strain. Note that binary plasmid pWISE0 lacks Pt-virD2 which is now part of virD operon on the virulence plasmid. FIG. 13D) Reconstituted virD operon in XYA105Pt Agrobacterium strain. Pt-virD2 can be any gene listed in Table 1.

DETAILED DESCRIPTION OF THE INVENTION

Tissue culture limitations in Arabidopsis nuclear gene transformation were overcome by using Agrobacterium to directly transform the female gametocyte, and identifying nuclear transgenic events by germinating the resulting seedlings on a selective medium. Here we describe reengineering of an Agrobacterium for T-DNA chloroplast delivery to directly transform the plastids in the female gametocyte. Side-stepping the tissue culture process eliminates the need for specialized expertise to practice plastid transformation in Arabidopsis and other plant species. Therefore, the invention described herein enables widespread applications of Arabidopsis plastid genome engineering which, combined with the available extensive genomic resources, will have a major impact on basic science and applications in plant biotechnology.

Agrobacterium tumefaciens is a plant pathogen which delivers tumor-inducing genes encoded in the transferred DNA (T-DNA) to the plant nucleus. The T-DNA has been disarmed and converted into a vector commonly used to obtain transgenic plants. In conventional approaches, T-DNA is delivered by linkage to a virulence (VirD2) protein, which guides the T-DNA through linkage to a Type IV secretion signal sequence (T4SS). Subsequently, the T-DNA is integrated in the plant nucleus. As described herein, the Agrobacterium delivery system has been redesigned to transfer the T-DNA to chloroplasts. We demonstrate that proteins can be directly exported from Agrobacterium to chloroplasts provided a C-terminal Type IV secretion signal (T4SS) is present for export to the plant cell and an N-terminal transit peptide for import into chloroplasts. Targets for reengineering include Vir proteins involved in T-DNA transfer. In order to promote chloroplast delivery of VirD2, the strong nuclear localization (NLS) signals are removed for effective reengineering of the Vir proteins for chloroplast delivery of T-DNA.

In the examples below, compositions and methods are provided for the rapid and efficient reengineering of Agrobacterium and other plant species for T-DNA delivery to chloroplasts to directly transform the plastids in the female gametocyte. Direct transformation of plastids in the female gametocyte eliminates tissue culture as the bottleneck of chloroplast transformation.

Definitions

By “T-DNA” is meant the transferred DNA or T-DNA of an Agrobacterium tumefaciens Ti plasmid or from an Agrobacterium rhizogenes Ri plasmid, or a derivative thereof. The T-DNA may comprise an entire T-DNA, but need only comprise the minimal sequences required in cis for transfer (i.e., the right and the left T-DNA border sequences). T-DNA delivery to germline cells would enable chloroplast transformation in all crops in which direct transformation of germline cells is feasible by the floral dip protocol.

The invention includes plant transformation vectors for use in Agrobacterium-mediated transformation of chloroplasts in plants. The vectors of the present invention have the pVS 1 replication origin (Heeb et al., 2000), but the replication origin may be replaced by other replication origins from the repABC family in Agrobacterium in certain embodiments. Also, vectors without an Agrobacterium-maintaining replication origin are capable of maintaining themselves in Agrobacterium with sequences in the T-DNA that can be integrated into either the Ti plasmid in A. tumefaciens, Ri plasmid in A. rhizogenes or Agrobacterium chromosome through homologous recombination. This results in the same effect of a reduction in non T-DNA sequence insertion and an increase in low copy number transformation events, since the chromosome, Ti or Ri plasmid is present in a single copy. When Rhizobia or non Agrobacterium species are used for plant transformation, T-DNA containing nucleic acid of interest can be integrated into the chromosome or into repABC plasmids in the host bacteria, which can produce the same effect as Agrobacterium does.

A vector of the present invention may comprise at least one of Agrobacterium Ti plasmid right border or left border region. The vectors may comprise at least one pair of borders. The vectors can include four pairs of borders, but the vector often comprise one or two pairs.

The vectors of the present invention may further comprise a coding region for a selectable marker for the maintenance in bacterial hosts. Coding regions for selectable markers include Spec/Strp that encodes for Shigella flexneri aminoglycoside adenyltransferase (aadA) conferring resistance to spectinomycin or streptomycin (Chinault et al. 1986), or a gentamycin (Gm, Gent) selectable marker gene from a Tn21-like multiresistance transposon (Wohlleben et al., 1989). Other resistance genes include carbenecillin, ampicillin, and kanamycin resistance genes. Others are known and may be readily used in the present invention by those of skill in the art.

The vectors of the present invention may also comprise a coding region for a plant selectable marker gene, which is typically located in T-DNA, to select transformed plant cells with the corresponding selection agent. The plant selectable marker may provide resistance to a positive selection compound, for example, antibiotic resistance (e.g., streptomycin/spectinomycin, kanamycin, chloramphenicol, tobramycin, gentamycin, etc.), or herbicide resistance (e.g., including but not limited to: glyphosate, Dicamba, glufosinate, sulfonylureas, imidazolinones, bromoxynil, dalapon, cyclohexanedione, protoporphyrinogen oxidase inhibitors, and isoxaflutole herbicides). Polynucleotide molecules encoding proteins involved in herbicide tolerance are known in the art and can also be introduced into target plant cells using the compositions and methods of the invention.

In addition to a plant selectable marker, in some embodiments it may be desirable to use a reporter gene. In some instances, a reporter gene may be used with or without a selectable marker. Reporter genes are genes that are typically not present in the recipient organism or tissue and typically encode for proteins resulting in some phenotypic change or enzymatic property.

Preferred reporter genes include the beta-glucuronidase (GUS) of the uidA locus of E. coli, the chloramphenicol acetyl transferase gene from Tn9 of E. coli, the green fluorescent protein from the bioluminescent jellyfish Aequorea victoria, and the luciferase genes from firefly Photinus pyralis or mScarlet. An assay for detecting reporter gene expression may then be performed at a suitable time after said gene has been introduced into recipient cells. A preferred such assay entails the use of split GFP.

In some embodiments, the vectors of the present invention comprise one replication origin for maintenance in E. coli. These origins of replication may be derived, for example, from pBR322 or from pUC. One example of such an origin of replication is ColE1. The vectors of the present invention can include any origin of replication which maintains at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, or 100 or more plasmid copies in E. coli. In some embodiments, the ori maintains a higher copy number in E. coli (e.g., greater than 5, 10, 15, 20, 25, or 30, and in some further embodiments, greater than 10, 15, 20, 25 or 30). Such high copy number vectors make it easier to amplify the amount of DNA available for transformation.

As used herein “operably linked” includes reference to a functional linkage between two sequences in a nucleic acid construct, for example, a promoter and a second sequence, wherein the promoter sequence initiates and mediates transcription of the DNA sequence corresponding to the second sequence. Generally, operably linked means that the nucleic acid sequences being linked are contiguous and, where necessary to join two protein coding regions, contiguous and in the same reading frame.

As used herein “promoter” includes reference to a region of DNA involved in recognition and binding of RNA polymerase and other proteins to initiate transcription.

The invention discloses novel methods of delivering proteins of interest directly to plant organelles. Because the protein of interest is transferred directly into the plant cell, the potential negative effects which can result from the transient or stable introduction of genetic material are avoided. Another benefit of the invention is that a protein of interest can be directly delivered to a plant cell in conjunction with stable incorporation of a transgene. This allows for a broad range of utilities from increasing the efficiency of plant cell transformation to enhancing agroinfection.

In addition, the methods of the invention can be used to test the efficacy of visible selectable markers by transferring chimeric VirD fusions with peptides derived from proteins such as GFP as described in the split GFP assay shown in FIG. 7 . The visible markers could also be used in the system to test changes in protocols that would enhance transfer of molecules to various plant cells, or cells or tissues of recalcitrant species.

As used herein, the term “plant” includes reference to whole plants, plant organs (e.g., leaves, stems, roots, etc.), seeds and plant cells and progeny of same. Plant cell, as used herein includes, without limitation, plant cells within or isolated from seeds, suspension cultures, embryos, meristematic regions, callus tissue, leaves, roots, shoots, gametophytes, sporophytes, pollen, and microspores.

Proteins delivered to plant cells using the Agrobacterium-mediated method will generally be localized to the nucleus of the plant cell. Additional modifications to the protein could result in the targeting to other specific subcellular locations within the plant cell. For example, “signal” or “transit” peptides are capable of targeting a polypeptide to the chloroplast, as known in the art. It is recognized that in the Agrobacterium-mediated method of protein delivery to plant cells, the signal peptide would be added to the polypeptide of interest in the bacterium. More specifically, the modified Agrobacterium containing a replicon encoding a protein which has a T4SS at its C-terminus to facilitate the movement of proteins from Agrobacterium to the plant cell and an N-terminal “signal sequence” or “transit peptide” to target the polypeptide of interest to a specific subcellular location in the plant cell.

Known chloroplast transit peptides include, without limitation, the chloroplast small subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco); 5-enolpyruvyl)shikimate-3-phosphate synthase (EPSPS); tryptophan synthase; plastocyanin; chorismate synthase; and the light harvesting chlorophyll a/b binding protein (LHBP).

The DNA construct of the present invention may be introduced into the genome of a desired plant host by a suitable Agrobacterium-mediated plant transformation method. Several Agrobacterium species mediate the transfer of T-DNA, described above, that can be genetically engineered to carry any desired piece of DNA into many plant species. Recently, non-Agrobacterium systems are coming to the fore for plant transformation, specifically Ensifer adhaerens, Ochrobactrum haywardense and Rhizobium etli (Rathore DS, Mullins E , 2018).

Other methods for introducing DNA into cells may also be used. These methods are well known to those of skill in the art and can include chemical methods and physical methods such as microinjection, electroporation, and micro-projectile.

Plant cell regeneration techniques rely on manipulation of certain phytohormones in a tissue culture growth medium, also typically relying on a biocide and/or herbicide marker that has been introduced together with the desired nucleotide sequences. Choice of methodology with suitable protocols being available for hosts from Leguminosae (alfalfa, soybean, clover, etc.), Umbelliferae (carrot, celery, parsnip), Cruciferae (cabbage, radish, canola/rapeseed, etc.), Cucurbitaceae (melons and cucumber), Gramineae (wheat, barley, rice, maize, etc.), Solanaceae (potato, tobacco, tomato, peppers), various floral crops, such as sunflower, and nut-bearing trees, such as almonds, cashews, walnuts, and pecans. Such regeneration techniques are known to those of skill in the art. Methods and compositions for transforming plants by introducing a transgenic DNA construct into a plant genome in the practice of this invention can include any of the well-known and demonstrated methods. For example, Agrobacterium-mediated transformation as illustrated in U.S. Pat. Nos. 5,824,877; 5,591,616; and 6,384,301, all of which are incorporated herein by reference.

The present invention may be used for delivery of proteins or modulatory DNA/RNAs to any plant species, including, but not limited to, corn (Zea mays), Brassica sp. (e.g., B. napus, B. rapa, B. juncea), particularly those Brassica species useful as sources of seed oil, alfalfa (Medicago sativa), rice (Oryza sativa), rye (Secale cereale), sorghum (Sorghum bicolor, Sorghum vulgare), millet (e.g., pearl millet (Pennisetum glaucum), proso millet (Panicum miliaceum), foxtail millet (Setaria italica), finger millet (Eleusine coracana)), sunflower (Helianthus annuus), safflower (Carthamus tinctorius), wheat (Triticum aestivum), soybean (Glycine max), tobacco (Nicotiana tabacum), potato (Solanum tuberosum), peanuts (Arachis hypogaea), cotton (Gossypium barbadense, Gossypium hirsutum), sweet potato (Ipomoea batatus), cassava (Manihot esculenta), coffee (Coffea spp.), coconut (Cocos nucifera), pineapple (Ananas comosus), citrus trees (Citrus spp.), cocoa (Theobroma cacao), tea (Camellia sinensis), banana (Musa spp.), avocado (Persea americana), fig (Ficus casica), guava (Psidium guajava), mango (Mangifera indica), olive (Olea europaea), papaya (Carica papaya), cashew (Anacardium occidentale), macadamia (Macadamia integrifolia), almond (Prunus amygdalus), sugar beets (Beta vulgaris), sugarcane (Saccharum spp.), oats, barley, vegetables, ornamentals, and conifers.

Vegetables include tomatoes (Lycopersicon esculentum), lettuce (e.g., Lactuca sativa), green beans (Phaseolus vulgaris), lima beans (Phaseolus limensis), peas (Lathyrus spp.), and members of the genus Cucumis such as cucumber ( C. sativus), cantaloupe ( C. cantalupensis), and musk melon ( C. melo). Ornamentals include azalea (Rhododendron spp.), hydrangea (Macrophylla hydrangea), hibiscus (Hibiscus rosasanensis), roses (Rosa spp.), tulips (Tulipa spp.), daffodils (Narcissus spp.), petunias (Petunia hybrida ), carnation (Dianthus caryophyllus), poinsettia (Euphorbia pulcherrima), and chrysanthemum. Conifers that may be employed in practicing the present invention include, for example, pines such as loblolly pine (Pinus taeda), slash pine (Pinus elliotii), ponderosa pine (Pinus ponderosa), lodgepole pine (Pinus contorta), and Monterey pine (Pin us radiata); Douglas-fir (Pseudotsuga menziesii); Western hemlock (Tsuga canadensis); Sitka spruce (Picea glauca); redwood (Sequoia sempervirens); true firs such as silver fir (Abies amabilis) and balsam fir (Abies balsamea); and cedars such as Western red cedar (Thuja plicata) and Alaska yellow-cedar (Chamaecyparis nootkatensis). Preferably, plants of the present invention are crop plants (for example, corn, alfalfa, sunflower, Brassica, soybean, cotton, safflower, peanut, sorghum, wheat, millet, tobacco, etc.), more preferably corn and soybean plants, yet more preferably corn plants.

Kits Comprising for Practicing the Methods for Agrobacterium Mediated Transformation of Chloroplasts

The invention also provides a kit for comprising materials and reagents useful for practice of the methods of the present invention.

Suitable pAG-CHL binary vectors (FIG. 11 ) for practicing the invention are pAG-CHL-1 [SEQ ID NO 29], where the Pt-VirD2-1 [SEQ ID NO 27] is blunted to the right of RB; pAG-CHL- 2, where Pt-VirD2-2 [SEQ ID NO 28] is blunted to the right of RB; pAG-CHL- 3, where Pt-VirD2-3 [SEQ ID NO 30] is blunted to the right of RB; pAG-CHL- 4, where Pt-VirD2-4 [SEQ ID NO 31] is blunted in the to the right of RB.

A suitable Agrobacterium strain carrying a virulence plasmid lacking the T-DNA and a virD2 gene, is XYA105ΔvirD2. The Pt-VirD2 protein can nick the T-DNA at the borders and guide it to plastids (e.g. chloroplasts) subsequent to transfer to the plant cell. Pt-VirD2 plasmids are incorporated on the binary plasmid. These plasmids will be made part of the virD operon carried on the helper plasmid. The reconstituted virD operon will comprise Pt-VirD2 variants, such as [SEQ ID NO 1], [SEQ ID NO 31], [SEQ ID NO 42] and [SEQ ID NO 43].

Other reagents include, without limitation, media and selection agents, described herein useful for the production of the transplastomic plants of the invention.

The following materials and methods are provided to facilitate the practice of the present invention.

Plant materials and growth conditions. Nicotiana tabacum cv. Petit Havana Nt-pSS33 is a transplastomic tobacco plant, carrying the excisable, attB/attP flanked bar^(au) gene in the plastid genome (Tungsuchat-Huang et al., 2010). The plants were grown in the greenhouse with supplemental lighting (16 hrs).

Vector construction. Agro-Int gene in plasmid pJCD6B is based on a pPZP200 binary vector derivative (Hajdukiewicz et al., 1994), in which the BclI restriction site was converted into a StuI site. The T-DNA borders have been removed by digestion with StuI/ScaI restriction enzymes, and replaced with the Agro-Int gene consisting of the virE promoter (Winans et al., 1987) (GenBank: X06826.2), the tobacco Rubisco small subunit transit peptide (Gnanasambandam et al., 2007) fused with the phiC31 integrase (AJ006589.3) at the N-terminus and 37 amino acids of the virF T4SS signal at the C-terminus, followed by the virF terminator (Melchers et al., 1990, Vergunst et al., 2005). Removal of the transit peptide and both, the transit peptide and the T4SS signal, yielded binary plasmids pAMland pAM2, respectively.

Agroinfiltration. Agrobacterium strain EHA105 (Hood et al., 1993) was transformed with the pAM1 (TP-Int), pAM2 (Int), or pJCD6B (Agro-Int) binary vectors described herein or pKO117 Nuclear-Int vector (Lutz et al., 2004). Infiltration of leaves with syringe was carried out as described (Sparkes et al., 2006). It was important to choose fully extended leaves of young plants before flower buds appeared. The Agrobacterium strains were inoculated into 30 mL YEB medium supplemented with 100 mg/L spectinomycin and 25 mg/L chloramphenicol and grown overnight at 28° C. to a final OD₆₀₀ of 0.5. The cultures were centrifuged at 4,200 g for 10 min. The cells were resuspended in 3 mL of the infiltration medium (250 mg D-glucose, 5 mL of 500 mM MES, 5 mL of Na₂PO₄·7H₂O, 50 µL of 100 µL of 100 mM acetosyringone, dH₂O up to 50 mL final volume). To wash the cells, the cell suspension was centrifuged again and then resuspended in 3 mL of the infiltration medium. The final OD₆₀₀ was adjusted to 1 for infiltration. Leaf sections (1 cm²) were collected daily for four days after infiltration (FIG. 3D.

PCR and DNA gel blot analyses. Total cellular DNA was isolated using the CTAB protocol. Excision of the target sequence was confirmed by PCR using total cellular DNA as template and primers AM102 (5′-GTTGAGTCGATACTTCGGCG-3′) (SEQ ID NO: 48) and AM103 (5′-GGCACCAGTCCTACATTTTG-3′) (SEQ ID NO: 49). The primer positions are shown in FIG. 3B. PCR amplicons were sequenced to verify the attR junction sequence (FIG. 3C). PCR was performed in a 20 µL reaction mixture containing 1.3 µg of total plant cellular DNA, 0.5 µM of primers AM102 and AM103, 1xJumpStart™REDTaq® ReadyMix™ Reaction Mix (SIGMA-ALDRICH, St. Louis, MO, Cat. No. P0982 ). PCR parameters were as follows: one cycle at 95° C. for 3 min, 30 cycles at 95° C. for 30 s, 57° C. for 30 s, 72° C. for 30 s, and one cycle at 72° C. for 10 min.

DNA gel blot analysis was also performed to detect excision of the target sequence (Tungsuchat-Huang et al., 2010). Total cellular DNA was digested with the BamHI restriction enzyme and probed with the 2.1 kb ApaI-BamHI ptDNA fragment.

The following examples are provided to illustrate certain embodiments of the invention. They are not intended to limit the invention in any way.

Example I Split GFP Assay to Visualize Engineered Vird2 Movement to Chloroplasts

We have shown direct export of the integrase site-specific recombinase by the excision of target sequences in chloroplasts. As a follow-up, we confirmed that VirD2, the protein guiding the T-DNA from Agrobacterium to the plant nucleus can also be re-targeted to chloroplasts. For this, we have used the split GFP assay (Cabantous et al., 2005). In the assay a small part of GFP (GFP11; RDHMBLHEYBNAAGIT) (SEQ ID NO: 50) is incorporated in the VirD2 protein (VirD2-11) and when it is transported from Agrobacterium (or the cytoplasm) to chloroplasts it complements a GFP 1-10 protein [SEQ ID NO 5] lacking the small peptide (GFP 11) (FIG. 4 ). Localization of the engineered VirD2-11 to chloroplasts is detected by fluorescence under a confocal microscope of complementing GFP parts.

Split GFP Complementation In E. Coli

We tested complementation of VirD2-11 variants and GFP 1-10 in E. coli by expressing GFP 1-10 in a pUC plasmid carrying a spectinomycin resistance marker (pAM305 [SEQ ID NO 5]) and VirD2-11 in a PET28a vector carrying a kanamycin resistance marker. Selection for spectinomycin resistance and kanamycin resistance ensured that both plasmids were present in E. coli. The VirD2-11 plasmids expressed a variant lacking the transit peptide (TP) but had the five SSU amino acids at the N-terminus, the form that will be present when the TP is cleaved off after import in chloroplasts. Some of the VirD2 variants carried the GFP 11 repeat at the N-terminus between the 5 SSU amino acids and the VirD2 N-terminus (pAM31 [SEQ ID NO 8]; pM32 [SEQ ID NO 9]); others between the truncated VirD2 C-terminus and the VirF T4SS (pAM33 [SEQ ID NO 10], AM34 [SEQ ID NO 11], pAM35 [SEQ ID NO 12] and pAM36 [SEQ ID NO 13]). Plasmids pAM32, pAM34, pA35 and pAM36 carry a linker sequence adjacent to the 16 aa GFP 11 peptide. The schematic maps of the plasmids used for the split GFP assay in E. coli are shown in FIG. 5 .

Each of the VirD2-11 proteins complemented GFP 1-10, indicated by fluorescence upon illumination with UV light (UVL-56, 366 nm Black-Ray Lamp, UVP Upland, CA 91786) (FIG. 6 ). The positive control was plasmid pAM29 [SEQ ID NO 6], expressing sulfite-reductase-GFP11 (Cabantous et al., 2005). The negative control was pAM30 [SEQ ID NO 7], Pt-VirD2 without GFP 11.

Split-GFP Complementation in Chloroplasts From Nuclear Genes

A split-GFP assay in chloroplasts when both GFP-1-10 and the Pt-VirD2 variants are expressed from nuclear genes can also be performed. The assay is schematically shown in FIG. 7 . GFP1-10 gene is cloned in a 35S promoter/ocs terminator cassette in plasmid pM407, a pBIN19 vector derivative [Seq. ID 14](Bevan, 1984). The GFP1-10 gene was introduced into the tobacco nucleus by standard protocols, selecting for kanamycin resistance. The gene was transcribed in the nucleus, its mRNA translated in the cytoplasm and the GFP 1-10 protein imported into chloroplasts where the transit peptide (RP) is cleaved off. GFP 1-10 is not fluorescent under UV light.

Pt-VirD2-11 plasmids are a series of plastid VirD2 variants, which carry a complementing GFP11 fragment in a pPZP 221 binary plasmid (Hajdukiewicz et al., 1994). The Pt-VirD2-11 genes encode proteins similar to those tested in XE. coli (FIG. 5 ), other than having the transit peptide sequence. The genes are cloned in a ubiquitin promoter/terminator cassette (Liu et al., 2015). The genes for the Pt-VirD2-11 variants are introduced into the tobacco nucleus of GFP 1-10 transgenic plants, where the genes are transcribed in the nucleus, their mRNA is translated in the cytoplasm and the Pt-VirD2-11 protein is imported in the chloroplasts where it complements GFP 1-10, indicating successful re-targeting of VirD2 to chloroplasts. The targeting region of Pt-VirD2-11 plasmids is shown in FIG. 8 . The DNA sequence of plasmids is also available: pJF7 [SEQ ID NO 15], pJF8 [SEQ ID NO 16], pJF9 [SEQ ID NO 17], pJF10 [SEQ ID NO 18], pJF11 [SEQ ID NO 19] and pJF12 [SEQ ID NO 20].

Agrobacterium-Mediated Chloroplast Transformation

To accomplish Agrobacterium-mediated chloroplast transformation we had to develop a new toolkit, because the markers in the current toolkit are not compatible with the Agrobacterium strain lacking VirD2. The new constructs we developed providing the new tools necessary for practice Agrobacterium-mediated chloroplast transformation are described in detail below.

XYA105ΔvirD2 Agrobacterium Strain

In certain embodiments, to avoid competition between chloroplast and nuclear targeting, we utilize an Agrobacterium strain lacking the virD2 gene (XYA105ΔvirD2). This strain is the derivative of the EHA101 agrobacterium strain, a C58 nopalin strain carrying the pTiBo542 virulence plasmid lacking the transferred DNA (T-DNA) (Hood et al., 1986). DNA sequence of plasmid pTi_EHA101 is deposited in GenBank (KY000035.1). The kanamycin resistance gene in pEHA101 was deleted (Hood et al., 1993), then the left-T-DNA border still present on the virulence plasmid was removed to obtain plasmid XYA105 (Li et al., 2020). The virD2 gene in XYA105 was subsequently deleted to obtain XYA105ΔvirD2 (Li et al., 2020). The deleted sequence is shown as [SEQ ID NO 21].

pAG Binary Backbone Plasmid Family

To adjust to the markers in XYA105, we constructed a new binary Agrobacterium binary vector family based on the pAG backbone with kanamycin resistance as the bacterial marker. The backbone of pAG serves as the foundation for both the chloroplast vector pAGC and the nuclear vector pAGN. The kanamycin resistance gene is described as part of pUK-S expression vector encoding APH(3′)-I family aminoglycoside 0-phosphotransferase (WP_099588326; AF053406.1)(Krieg et al., 1998). We modified the coding region by removing two NsiI restriction sites and one NruI restriction site in the coding region by silent mutagenesis and converted the kanR gene into an NsiI-StuI fragment by adding the restriction sites at the ends [SEQ ID NO 30]. The spectinomycin resistance gene in the pPZP200 binary vectors could be replaced with the synthetic StuI-NsiI fragment purchased from BlueHeron Biotechnology.

The pAG T-DNA region was designed as follows (FIG. 9 ). The LB is the 25 bp pTiT37 left border repeat, as in the pPZP vectors (Hajdukiewicz et al., 1994). We have used two 25-bp repeats [SEQ ID NO 51] with a spacer [SEQ ID NO 52], because two repeats improved the correct processing of the T-stand, reducing the transfer of vector sequences beyond the LB (Kuraya et al., 2004, Podevin et al., 2006, Thole et al., 2007). The RB also derives from pTiT37 [SEQ ID NO 53], as in the pPZP binary vectors (Hajdukiewicz et al., 1994). For efficient T-DNA transfer, sequences termed overdrive [SEQ ID NO 54], that are binding sites for VirC1, are also included (Toro et al., 1989). Because the pTiT37 plasmid does not contain a well-defined overdrive sequence, we have utilized the pTiA6 overdrive (Peralta et al., 1986, Thole et al., 2007). Between the LB and RB we included the PacI, I-SecI and AscI restriction sites to facilitate insertion of sequences for T-DNA transfer.

Four variants of the pAG backbone were created differing in the position of pUC multiple cloning sites relative to the T-DNA. In version a (pAGa) the multiple cloning site (MCS) is adjacent to the T-DNA right border (RB) and the restriction sites are as in pUC18 (From EcoRI to HindIII); in version b (pAGb) the MCS is also adjacent to the RB, but the restriction sites are in the reverse order (from HindIII- to EcoRI); in c (pAGc) and d (pAGd) the restriction sites are outside the LB and are arranged as in pUC18 (pAGc) and in reverse order (pAGd) for cloning flaxibility. The map of the T-DNA region of the four pGA vectors is shown in FIG. 9 . The DNA sequences of the vectors are: pAGa [SEQ ID NO 22], pAGb [SEQ ID NO 23], pAGc [SEQ ID NO 24] and pAGd [SEQ ID NO 25].

The pAGC Chloroplast Transformation Vector

The pAGC chloroplast transformation backbone is based on the pAGa-pAGd vector backbones and targets insertion between the trnV gene and 3′-rpsl2/7 operon in the inverted repeat region of the tobacco chloroplast genome. The targeting regions contain nucleotides 104,087-101,147 of the tobacco ptDNA (NC_001879.2) where one of the HincII site was converted into a cloning site, using SnaBI and HpaI enzymes. The position of the insertion site is the same as in the pPRV chloroplast transformation vectors (Zoubenko et al., 1994). To facilitate identification of transplastomic events a dicistronic aadA-gfp operon (MF461355) was inserted into the cloning site, so that transplastomic events can be selected by spectinomycin resistance, and non-transplastomic spectinomycin resistant clones eliminated by the lack of GFP accumulation (Yu et al., 2017, Yu et al., 2020). Spectinomcyin resistance of non-transplastomic clones can be due to nuclear insertion of the marker gene cassette and spontaneous mutations in the 16S rRNA gene. The map of binary chloroplast transformation vector pAGCa carrying the dicistronic aad-gfp cassette [SEQ ID NO 26] is shown in FIG. 10 . The schematic map of Pt-VirD2-1 gene in a VirD promoter/VirF terminator cassette [SEQ ID NO 27] is shown above the MCS. Pt-VirD2-2 can also be expressed in a VirE promoter/VirF terminator cassette [SEQ ID NO 28] for efficiency in chloroplast transformation.

Chloroplast Transformation by Agrobacterium Using the pAG-CpTEST Chloroplast Transformation Vector

For successful chloroplast transformation by Agrobacterium, a Pt-VirD2 gene should be inserted in the multiple cloning site. To obtain a properly regulated plastid Pt-VirD2 gene, the Pt-VirD2 coding sequence can be inserted into a VirD operon promoter/VirF terminator cassette and the cassette cloned in the pAGCa multiple cloning site to obtain an agrobacterium binary vector, pAG-CpTEST [SEQ ID NO 26]. The vector can then be introduced by electroporation into a XYA105ΔvirD2 strain and used for cocultivation using a protocol modified for chloroplast transformation.

For chloroplast transformation by Agrobacterium, leaf sections of sterile plants are infiltrated with Agrobacterium, and then the leaf sections are transferred to a selective medium containing 500 mg/L spectinomycin to select for transplastomic events and 500 mg/L carbenicillin to control Agrobacterium proliferation.

Agroinfiltration of tobacco leaves is carried out as described (Kapila et al., 1997, Lutz et al., 2006). Briefly, Agrobacterium strain XYA105ΔvirD2 strain is transformed with vector pAGC-1. The Agrobacterium strains were inoculated into 100 mL yeast extract beef medium supplemented with 50 mg/L kanamycin and 25 mg/L chloramphenicol and grown overnight at 28° C. to a final OD₆₀₀ = 0.8. The cultures were sedimented by centrifugation, and resuspended in MMA medium (MS salts, 10 mM of MES, 20 g/L sucrose, pH 5.6, and 200 µM acetosyringone) to a final OD₆₀₀ of 2.4. The Agrobacterium suspension was kept at 22° C. for one hour, then used for Agroinfiltration of sterile leaves (1 cm²). It is important to select fully expanded leaves Agroinfiltration. Vacuum was applied at 5 Torr for 20 min. Leaf sections were placed on RMOP medium for co-cultivation. Placing the infiltrated leaf disks on a filter paper helps to control Agrobacterium growth during cocultivation. After 2 days of co-cultivation, leaves are transferred to RMOP medium supplemented with 500 mg/L carbenicillin to inhibit Agrobacterium cell division and 500 mg/L spectinomycin to select for transplastomic events. Transplastomic events are identified as green proliferating calli and shoots on a selective medium that suppresses growth and greening of wild type tobacco cells (Svab and Maliga, 1993).

pAG-CHL Vectors for Introduction of Genes of Interest in the Plastid Genome

In vector pAG-CpTEST-1, (also referred to as Pwise herein) the Pt-VirD2-1 gene is flanked by restriction sites, so that alternative Pt-VirD2 variants can be readily cloned into the vector. The resulting vectors can also be tested for improving the efficiency T-DNA transfer. The tobacco SSU transit peptide and truncated VirD2 fusion protein has been shown to be efficiently processed by VirD2 in a nicking assay (Matsuoka, 2015), but T-DNA delivery to chloroplasts may be improved by using alternative transit peptide sequences (Armbruster et al., 2009, Sjuts et al., 2017). When suitable variants are identified, the Pt-VirD2 variant will be inserted without any major cloning sites, and the targeting region will be modified to include a multiple cloning site for the insertion of genes of interest (vector pAG-CHL-1; SEQ ID NO 29; FIG. 11 ).

Floral Dip Protocol

The Arabidopsis thaliana floral dip protocol is caried out as described (Clough and Bent, 1998) and modified (Zhang et al., 2006). The Arabidopsis plants (~nine per pot) are grown in soil until they bolt and 80% start to produce first floral inflorescences. The first shoots are then cut down to 3 cm to provide a mechano-stimulus for a uniform second batch of shoots with more branching (7-10 days). When the second batch of shoots are 15-20 cm, they are ready for the floral dip. To prepare Agrobacterium cultures for floral dip, cultures are streaked on fresh LB medium with antibiotics. 2mL liquid LB is then inoculated with 2-3 colonies for overnight (~16 hours) culture at 28° C. with shaking at 220-230 rpm in a 1000 mL flask. Agrobacterium cells are collected by centrifugation, then resuspended in 1L ddH₂O, 50 g sucrose, 0.48 g MgCl₂, 300-500 µl Silwett L-77 added just prior to resuspending Agrobacterium). The flowers are briefly dipped (30-60 seconds) in the Agrobacterium solution with gentle swirling of the the inflorescences. The pots are then laid sideways and the plants covered with a plastic cover to preserve humidity overnight. The plants are placed upright in a clean tray and allowed to flower.

Seed is collected and germinated on RM medium modified for Arabidopsis (Yu et al., 2017; Yu et al., 2019). Seedlings with green sectors that fluoresce are then identified under UV light. The seedlings are then transferred to soil and seeds collected from branches with GFP sectors. The seed is then germinated in the presence of spectinomycin (50 mg/L) plants having uniformly transformed plastid genomes selected.

Example II T-DNA Markers in Agrobacterium Including Antibiotic Resistance

The T-DNA in the T-complex is assumed to be single stranded (Stachel et al., 1986). As described in Example I, Agrobacterium binary vectors can deliver several kb-long T-strands. Smaller T-DNA strands are more likely to be imported into chloroplasts via the translocons that evolved for protein import from the cytoplasm. The shortest T-DNA strands that can be used as markers for chloroplast transformation is approximately 60 nt. Antibiotic resistance is a preferred marker; however, many other marker options are known to those skilled in the art.

Selection for Antibiotic Resistance by Conversion of Native Plastid Genes With T-DNA Carrying Short Oligonucleotides

Early evidence for plastid transformation was obtained by selection for spectinomycin resistance encoded in 16S rRNA which is present in the plastid genome. The wild-type sensitive allele can be converted into a resistant one by a short single-stranded DNA transported by the Pt-VirD2 protein. There are several point mutations that disrupt a short stem structure in the 16S rRNA (Svab and Maliga, 1991). 60 nt-long synthetic oligonucleotides containing point mutations can be cloned as PacI/AscI fragments to replace the plastid targeting region in the pAG-CHL-1 Agrobacterium binary vector (FIG. 11 ). DNA sequences of PacI/AscI fragments with oligonucleotides conferring streptomycin resistance in the 16S rRNA include [SEQ ID NO 33] [SEQ ID NO 34] and [SEQ ID NO 35]. PacI/AscI fragments conferring spectinomycin resistance to the 16S rRNA include [SEQ ID NO 36], [SEQ ID NO 37], [SEQ ID NO 38], [SEQ ID NO 39] and [SEQ ID NO 40]. Single nucleotide exchanges occur randomly, yielding identical phenotypes. The Agrobacterium cocultivation and selection for spectinomycin (or streptomycin) resistance predominantly yielding the mutation that is present in the vector strongly supports gene conversion by T-DNA as the source of spectinomycin (streptomycin) resistance.

In an alternative approach, streptomycin resistance can also be conferred by introducing point mutations in rps12, a plastid-encoded ribosomal protein gene (Galili et al., 1989; Staub and Maliga, 1992; Hsu et al., 1993). Chloroplast transformants and spontaneous mutants could be distinguished via selection. The C to T mutation in codon 91 results in a Pro to Ser substitution, conferring streptomycin resistance in rps12. Silent mutations TTA to TTG or GGT to GGA in the adjacent codons do not result in amino acid substitutions. The point mutation resulting in streptomycin resistance will be included in the T-DNA, alongside with at least one of the adjacent silent mutations. Under these conditions, the presence of the silent mutation alongside with the mutation conferring streptomycin resistance. an event that is unlikely to occur spontaneously, provides irrefutable evidence that streptomycin resistance is due to gene conversion by the T-DNA. The sequence of the PacI/AscI fragment that yields streptomycin resistance and the TTA to TTG silent mutation in the adjacent codon is [SEQ ID NO: 41]. The vector with C271T changes Pro to Ser conferring streptomycin resistance and T276A is a silent mutation in [SEQ ID NO: 55]. An additional oligonucleotide that confers streptomycin resistance is SEQ ID NO 61, in which the C271T changes Pro to Ser conferring streptomycin resistance and two additional silent mutations create a KpnI site (Staub and Maliga, 1992).

Alternative Transit Peptides to Facilitate the Import of T-Complex by Pt-VirD2 Variants

Transit peptides facilitate the import of proteins which are targeted to chloroplasts. These peptides are cleaved off by a stromal processing peptidase (SPP) upon entry into the stroma (Sjuts et al., 2017). In certain embodiments, the Pt-VirD2 constructs have the tobacco Rubisco small subunit transit peptide (TP) [SEQ ID NO 1]. The tobacco Rubisco SSU is relatively small (XM_016585367; 180 aa). Plastidic homomeric acetyl-CoA carboxylase (ACC2, AT1G36180.1) is one of the largest proteins (2355 aa) targeted to chloroplasts and its transit peptide, is provided on ppdb.tc.cornell.edu and the PeptideAtlas. The mature protein starts with C76 or F77. Inclusion of the 6 amino acids of the mature ACC2 N-terminus, renders information on the exact cleavage site is irrelevant. It is expected that the ACC2 TP which normally imports large proteins, (SEQ ID NO: 42) will be highly efficient. A third TP that shows promise is a non-canonical stromal targeted protein in which the transit peptides are not processed by a SPP. For example, Arabidopsis mitogen-activated protein kinase kinase4 (AtMKK4) has a non-canonical transit peptide; its first 65 residues are necessary for chloroplast targeting (GenBank: NP_175577, NM_104044) (Samuel et al., 2008). PtMKK4-VirD2 [SEQ ID NO 43] comprising the Arabidopsis MKK4 transit peptide can also be used.

Construction of Agrobacterium Strains Carrying New Helper Plasmids With Pt-VirD2 as Part of the Vird Operon

The Pt-VirD2 plasmids are incorporated on the binary plasmid. These plasmids can also be made part of the virD operon carried on the helper plasmid. This can be accomplished using a sacB-based gene replacement strategy as illustrated in FIG. 12 (Hoang et al., 1998; Huang and Wilks, 2017; Li et al., 2020).

First, a tetracycline resistance gene will be replaced with a kanamycin resistance gene in pEX18Tc (GenBank Accession No. AF047519) (Available on the world wide web at novoprolabs.com/vector/Vgyytina) yielding plasmid pEX18Kan [SEQ ID NO 44]. For insertion into the virD operon, Pt-VirD2 genes were flanked by part of the virD1 gene and virD3 gene in vector pEX18Kan. The pEX18Kan derivatives will be introduced into XYA105ΔvirD2 strain. Agrobacterium first will be selected for kanamycin resistance to obtain cointegrated forms, then grown on sucrose to recover derivatives in which Pt-VirD2 was incorporated in the virD operon (FIG. 12 ). The vector for insertion of Pt-VirD2 with the Rubisco SSU-TP is [SEQ ID NO 45], with the ACC2-TP is [SEQ ID NO 46] and with the MKK4-TP is [SEQ ID NO 47].

FIG. 13 depicts chloroplast transformation using Agrobacterium. (A) Chloroplast transformation in a tobacco cell with the XYA105ΔvirD2 stain, carrying a pWISE binary vector. (B) The virD2 operon on the XYA105ΔvirD2 virulence plasmid. (C) Chloroplast transformation in a tobacco cell with a pWISE0 binary vector, using a pM&M105 Agrobacterium strain. (D) The Pt-virD2 gene on the pM&M105 virulence plasmid.

In case of the pWISE vectors (FIG. 13A), the binary plasmid encodes both the T-DNA and the Pt-virD2. An example is SEQ ID NO 29 (FIG. 11 ). Similar WISE vectors can be obtained by combining the pAGa, pAGb, pAGc and pAGd backbones (FIG. 9 ) with the vector sequences in Table 2 and Pt-VirD2 genes in Table 1.

When pWISE0 vectors are used in pM&M105 Agrobacterium strains (FIG. 13C), the pWISE0 binary vector may encode the T-DNA sequence listed in Table 2 only, because Pt-VirD2 is part of the virD operon.

TABLE 1 SEQ ID OF Pt-virD2 genes SEQ ID NO Promoter/Terminator Cassette Chloroplast Transit Peptide virD2 derivative T4SS 31 PvirD/TvirF Rubisco SSU+5aa Pt-VirD2 virF 27 PvirD/TvirF Rubisco SSU+5aa Pt-VirD2M virF 42 PvirD/TvirF ACC2+6aa Pt-virD2 virF 43 PvirD/TvirF mKK4 Pt-virD2 virF 32 PvirE/TvirF Rubisco SSU+5aa Pt-VirD2 virF 28 PvirE/TvirF Rubisco SSU+5aa Pt-VirD2M virF

TABLE 2 Chloroplast targeting region of pWISE vectors between PacI and AscI sites SEQ ID NO Targeting seq. Marker / selection Reporter gene or goi 26 trnV/3′rps12/7 (long) aadA gfp 29 trnV/3′rps12/7 (long) aadA 56 trnV/3′rps12/7 (short) aadA 33 rrn16 Streptomycin r. 34 rrn16 Streptomycin r. 35 rrn16 Streptomycin r. 36 rrn16 Spectinomycin r. 37 rrn16 Spectinomycin r. 38 rrn16 Spectinomycin r. 39 rrn16 Spectinomycin r. 40 rrn16 Spectinomycin r. 41 rps12 Streptomycin r. 55 rps12 Streptomycin r. 61 rps12 Streptomycin r.

Sequences useful for the practice of the present invention

SEQ ID NO 1 DNA sequence of Pt-VirD2. Coding sequence, tobacco SSU-TP+5aa fused with mutant minimal VirD2+T4SS signal SEQ ID NO 2 DNA sequence of integrase gene in binary vector pJCD6B. SEQ ID NO 3 Amino acid sequence of the phiC31 integrase in plasmid pJCD6. SEQ ID NO 4 DNA sequence of binary plasmid pJCD6B. SEQ ID NO 5 pAM305, with dicistronic aadA-gfp1-10 gene, for expression in chloroplasts. SEQ ID NO 6 pAM29. Sulfite-reductase-GFP11 fusion protein, in a T7 P/T cassette. SEQ ID NO 7 pAM30. Pt-VirD2 (without GFP1)1, in a T7 P/T cassette. SEQ ID NO 8 pAM31 SEQ ID NO 9 pAM32 SEQ ID NO 10 pAM33 SEQ ID NO 11 pAM34 SEQ ID NO 12 pAM35 SEQ ID NO 13 pAM36 SEQ ID NO 14 pAM407 SEQ ID NO 15 pJF7 SEQ ID NO 16 pJF8 SEQ ID NO 17 pJF9 SEQ ID NO 18 pJF10 SEQ ID NO 19 pJF11 SEQ ID NO 20 pJF12 SEQ ID NO 21 Deleted virD2 sequence in XYA105DvirD2 SEQ ID NO 22 DNA sequence of backbone vector pAGa SEQ ID NO 23 DNA sequence of backbone vector pAGb SEQ ID NO 24 DNA sequence of backbone vector pAGc SEQ ID NO 25 DNA sequence of backbone vector pAGd SEQ ID NO 26 DNA sequence of binary chloroplast transformation vector pAG-CpTEST Note dicistronic aadA-gfp marker genes. SEQ ID NO 27 The DNA sequence of Pt-VirD2 in a VirD promoter/VirF terminator cassette. The Pt-VirD2 has the tobacco SSU Transit peptide+5aa, the mutant minimal VirD2-T4SS signal. SEQ ID NO 28 The DNA sequence of Pt-VirD2 in a VirE promoter/VirF terminator cassette. The Pt-VirD2 has the tobacco SSU Transit peptide+5aa, the mutant minimal VirD2-T4SS signal. SEQ ID NO 29 DNA sequence of pAG-CHL-1 chloroplast binary transformation vector for introduction of transgenes (goi) into the plastid genome. The binary vector carries a Pt-VirD2 gene outside the T-DNA listed as SEQ ID No 27. SEQ ID NO 30 DNA sequence of kanamycin resistance gene in StuI-NsiI fragment. SEQ ID NO 31 The DNA sequence of Pt-VirD2-3 in a VirD promoter/VirF terminator cassette where Pt-VirD2 has the wild type NLS1. The Pt-VirD2-3 has the tobacco SSU Transit peptide+5aa, the wt minimal VirD2-T4SS signal. SEQ ID NO 32 The DNA sequence of Pt-VirD2-4 in a VirE promoter/VirF terminator cassette where Pt-VirD2 has the wild type NLS1. The Pt-VirD2-4 has the tobacco SSU Transit peptide+5aa, the wt minimal VirD2-T4SS signal. SEQ ID NO 33 DNA sequence introducing a C to T point mutation at nucleotide 473 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring streptomycin resistance SEQ ID NO 34 DNA sequence introducing a C to A point mutation at nucleotide 861 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring streptomycin resistance SEQ ID NO 35 DNA sequence introducing a C to T point mutation at nucleotide 861 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring streptomycin resistance SEQ ID NO 36 DNA sequence introducing a G to A point mutation at nucleotide 1013 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring spectinomycin resistance SEQ ID NO 37 DNA sequence introducing a A to C point mutation at nucleotide 1139 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring spectinomycin resistance SEQ ID NO 38 DNA sequence introducing a C to T point mutation at nucleotide 1140 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring spectinomycin resistance SEQ ID NO 39 DNA sequence introducing a G to A point mutation at nucleotide 1141 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring spectinomycin resistance SEQ ID NO 40 DNA sequence introducing a G to A point mutation at nucleotide 1335 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring spectinomycin resistance SEQ ID NO 41 DNA sequence introducing streptomycin resistance and a silent mutation in the tobacco rps12 coding sequence. Flanked by PacI and AscI restriction sites.. C271T changes Pro to Ser conferring streptomycin resistance. A270G is a silent mutation. SEQ ID NO 42 DNA sequence of Pt-ACC2-virD2 in a VirE promoter/VirF terminator cassette (P/T are in lower case) where Pt-ACC2-virD2 has A. thaliana ACC2 transit peptide+6aa fused with wt minimal VirD2 and T4SS signal. SEQ ID NO 43 DNA sequence of PtMKK4-virD2 in a VirE promoter/VirF terminator cassette (P/T are in lower case) where PtMKK4-virD2 has A. thaliana MKK4 transit peptide fused with wt minimal VirD2 and T4SS signal. SEQ ID NO 44 DNA sequence of pEX18Kan pEX18Tc (Accession no. AF047519) derivative with kanamycin resistance as selectable marker. SEQ ID NO 45 EcoRI-HindIII fragment of Pt-virD2 flanked with 500 bp of virD1 and 500 bp of virD3. The PtVirD2 has the tobacco SSU Transit peptide, the wt minimal VirD2-T4SS signal listed as SEQ ID NO 31. SEQ ID NO 46 EcoRI-HindIII fragment of PtACC2-virD2 flanked with 500 bp of virD1 and 500 bp of virD3. The PtACC2-VirD2 has the Arabidopsis ACC2 Transit peptide, the wt minimal VirD2-T4SS signal listed as SEQ ID NO 42. SEQ ID NO 47 EcoRI-HindIII fragment of PtMKK4-virD2 flanked with 500 bp of virD1 and 500 bp of virD3. The PtMKK4-VirD2 has the Arabidopsis MKK4 Transit peptide, the wt minimal VirD2-T4SS signal listed as SEQ ID NO 43. SEQ ID NO 48 AM 102 Primer SEQ ID NO 49 AM 102 Primer SEQ ID NO 50 GFP11 SEQ ID NO 51 Left Border sequence SEQ ID NO 52 Spacer between Left Border repeats SEQ ID NO 53 Right Border sequence SEQ ID NO 54 Overdrive sequence SEQ ID NO 55 DNA sequence introducing streptomycin resistance and a silent mutation in the tobacco rps12 coding sequence. Flanked by PacI and AscI restriction sites.. C271T changes Pro to Ser conferring streptomycin resistance. T276A is a silent mutation. SEQ ID NO 56. A variant of SEQ ID NO 29 (FIG. 11 ), with shorter plastid targeting sequences. Note that the PacI and AscI sites have been blunted. SEQ ID NO 57 The minimal attB sequence SEQ ID NO 58 The minimal attP sequence SEQ ID NO 59 The attL sequence SEQ ID NO 60 The attR sequence SEQ ID NO 61 Oligonucleotide confers streptomycin resistance and the silent mutations create a KpnI restriction site in the rps12 plastid gene.

> SEQ ID NO 1 DNA sequence of Pt-VirD2. Coding sequence, tobacco SSU-TP+5aa fused with mutant minimal VirD2+T4SS signal

atggcttcctcagttctttcctccgcagcagttgccacccgcagcaatgt tgctcaagctaacatggttgcacctttcactggccttaagtcagctgcct cattccctgtttcaaggaagcaaaaccttgacatcacttccattgccagc aacggcggaagagtgcaatgcatgCAGGTGTGGCCAGCGATGGCTCCCGA TCGGGCCcaagtaatcattcgcattgtgccaggaggtggaaccaagaccc ttcagcagataatcaatcagctggagtacctgtccacgaagggaaagctg gaactccagcgttcagcccggcatctcgatattcccgttccgccggatca aatccgtgagcttgcccaaagctgggttacggaggccgggatttatgacg aaagtcagtcagacgatgacaggcaacaagacttaacaacacacattatt gtgagcttccccgcaggtacggaccaaaccgcagcttatgaagcaagccg ggaatgggcagccgagatgtttgggtcaggatacgggggtggccgctata actatctgacagcctaccacgtggaccgcgatcatccacatttacatgtc gtggtcaatcgtcgggaacttctggggcaggggtggctgaaaatatccag gcgccatccccagctgaattatgacggcttacggaaaaagatggcagaga tttcacttcgtcacggcatagtcctggatgcgacttcccgagcagaaagg ggaatagcagagcgaccaatcacctatgctgaatatcgacgtCTTGAGCG GATGCTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCATTAAAA CGGCTCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGACCTCGA TCAACGCGCGGTCTATGA

>SEQ ID NO 2 DNA sequence of integrase gene in binary vector pJCD6B

tgcggaacatagaaagcgtcagagacgagacgcaccgtaagtgcgtgcta cattgtcctcggccgcacctaaagaccggggcagttgaaatccgattgtg atggcaactataagcgggtgacgaacagcacggattctagaatcatagac cgcgcgttgatcgaggtctgtccgccgacattaattccgctcgcgcatcg tcgtgagccgttttaatggatcgggctatgggccgaacttctgccataac ttcgagggcgcccgccgcgacgtcttccgtgccgtcctgggcgtcgtctt cgtcgtcgtcggtcggcggcttcgcccacgtgatcgaagcgcgcttctcg atgggcgttccctgccccctgcccgtagtcgacttcgtgacaacgatctt gtctacgaagagcccgacgaacacgcgcttgtcgtctactgacgcgcgcc cccaccacgacttagggccggtcgggtcagcgtcggcgtcttcggggaac cattggtcaaggggcagcttcggggcttcggcggcttcaagttcggcaag ccgctcttccgccccttgctgccggagcgtcagcgctgcctgttgcttcc ggaagtgcttcctgccaacgggtccgtcgtacgcgcctgccgcgcggtct tcgtacagctcttcaagggcgttcagggcgtcggcgcgctccgcaacaag gttcgcccgttcgccgctcttctcaggcgcctcagtgagcttgccgaagc gtcgggcggcttcccacagaagcgccaacgtctcttcgtcgccttcggcg tgcctgatcttgttgaagatgcgttccgcaacgaacttgtcgagtgccgc catgctgacgttgcacgtgccttcgtgctgcccaggtgcggacgggtcga ccaccttccggcgacggcagcggtaagagtccttgatcgattcttccccg cgcttcgaagtcatgacggcgccacactcgcagtacagcttgtccatcgc ggacagaatggcttgcccccgggaaagccccttgccgcgccccctgccgt ccaaccacgcctgaagctcataccactcagcgggctcgatgatcggtccg caatcaagctcgaccggccggagcgtgatcgggtcgcgctgaatgcggta accctcaatcttcgtggtcggcgtgccgtccggcttcttcttgtagatca cctcagcggcgaagcccgcaatacgcgggtcccgaaggattcgcataacg gttgccgggtcccaggcgcttgaagcggtcttcttcccaatcgtctcgcc ccgggtcggcacggcgtcagcgtccatgcgcttacaaagccccgtgatgc tgcccgggtgaatggcggcttgactgcccggcttgaagggaaggtgtttg tgcgtcttgatctcacgccaccaccaccggattacgtcgggctcgaactc gaagggtccggtaaggggagtggtcgagtgcgccagcttgttgatgacga cattgaccattcggccgttgcgcgtgatctccttcgtctccgaaacaagc tcgaagccgtaaggcgccttcccgccgacgtacccgcccaattcgcgctg aaggttcttcgtgtcgagaatcttcgccgacttcagcgaagattctttgt gcgacgcgtcgagccgcataatcaggtgaatcaggtccatgacgtttccc tgccggaagacgccttcctgagtggaaacaatcgtcacgcccagggcgag caattccgagacaatcggaatcgcgtccatgaccttcaggcgcgagaagc gcgacacgtcatagacaatgatcatgttgagccgcccggcgcggcattcg ttcaggatgcgttcgaactccgggcgctccgccgtcccgaacgccgacgt gcccggcgcttcgctgaaatgcccgacgaacctgaaccggcccccgtcgc gctcgacttcgcgctgaaggtcggccgccttgtcttcgttggcgctacgc tgtgtcgctgggcttgctgcgctcgaattttcgcgctcgcgcgactgacg gtcgtaagcacccgcgtacgtgtccatgctagccatggctggccacacct gcatgcattgcactcttccgccgttgctggcaatggaagtgatgtcaagg ttttgcttccttgaaacagggaatgaggcagctgacttaaggccagtgaa aggtgcaaccatgttagcttgagcaacattgctgcgggtggcaactgctg cggaggaaagaactgaggaagccatatgttctctcctgcaaaattgcggt tttccagaaaatcctctgcaacccgagataaagctgctttgctactttga gcaaagccgggggcggaactgttttgatcagtgcgaccactagacgaccc acttataaggtccgtcgtcagtaacctcttatcggaatttttaagtctcc gaccgggaagtctggcaagtgctcatatctccaaagtaccatccatactc gggtcttgaaacggatatcgcgtttcaactgcaatgagcaggcaataaat gatgccacaatcgacctgaaagggaggaactggaaagactggacgcacgc ggcctgactgtcgaggccatcgtatccctgtgagcattgttggtgacgag cagccg

>SEQ ID NO 3 Amino acid sequence of the phiC31 integrase in plasmid pJCD6

MASSVLSSAAVATRSNVAQANMVAPFTGLKSAASFPVSRKQNLDITSIAS NGGRVQCMQVWPAMASMDTYAGAYDRQSRERENSSAASPATQRSANEDKA ADLQREVERDGGRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLN MIIVYDVSRFSRLKVMDAIPIVSELLALGVTIVSTQEGVFRQGNVMDLIH LIMRLDASHKESSLKSAKILDTKNLQRELGGYVGGKAPYGFELVSETKEI TRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWREIKTHKHLPFKPG SQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRILRDP RIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAE WYELQAWLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDS YRCRRRKVVDPSAPGQHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETL ALLWEAARRFGKLTEAPEKSGERANLVAERADALNALEELYEDRAAGAYD GPVGRKHFRKQQAALTLRQQGAEERLAELEAAEAPKLPLDQWFPEDADAD PTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPIEKRASITW AKPPTDDDEDDAQDGTEDVAAGALEVMAEVRPIARSIKTAHDDARAELMS ADRPRSTRGL

>SEQ ID NO 4 DNA sequence of binary plasmid pJCD6B

agctttgcggaacatagaaagcgtcagagacgagacgcaccgtaagtgcg tgctacattgtcctcggccgcacctaaagaccggggcagttgaaatccga ttgtgatggcaactataagcgggtgacgaacagcacggattctagaatca tagaccgcgcgttgatcgaggtctgtccgccgacattaattccgctcgcg catcgtcgtgagccgttttaatggatcgggctatgggccgaacttctgcc ataacttcgagggcgcccgccgcgacgtcttccgtgccgtcctgggcgtc gtcttcgtcgtcgtcggtcggcggcttcgcccacgtgatcgaagcgcgct tctcgatgggcgttccctgccccctgcccgtagtcgacttcgtgacaacg atcttgtctacgaagagcccgacgaacacgcgcttgtcgtctactgacgc gcgcccccaccacgacttagggccggtcgggtcagcgtcggcgtcttcgg ggaaccattggtcaaggggcagcttcggggcttcggcggcttcaagttcg gcaagccgctcttccgccccttgctgccggagcgtcagcgctgcctgttg cttccggaagtgcttcctgccaacgggtccgtcgtacgcgcctgccgcgc ggtcttcgtacagctcttcaagggcgttcagggcgtcggcgcgctccgca acaaggttcgcccgttcgccgctcttctcaggcgcctcagtgagcttgcc gaagcgtcgggcggcttcccacagaagcgccaacgtctcttcgtcgcctt cggcgtgcctgatcttgttgaagatgcgttccgcaacgaacttgtcgagt gccgccatgctgacgttgcacgtgccttcgtgctgcccaggtgcggacgg gtcgaccaccttccggcgacggcagcggtaagagtccttgatcgattctt ccccgcgcttcgaagtcatgacggcgccacactcgcagtacagcttgtcc atcgcggacagaatggcttgcccccgggaaagccccttgccgcgccccct gccgtccaaccacgcctgaagctcataccactcagcgggctcgatgatcg gtccgcaatcaagctcgaccggccggagcgtgatcgggtcgcgctgaatg cggtaaccctcaatcttcgtggtcggcgtgccgtccggcttcttcttgta gatcacctcagcggcgaagcccgcaatacgcgggtcccgaaggattcgca taacggttgccgggtcccaggcgcttgaagcggtcttcttcccaatcgtc tcgccccgggtcggcacggcgtcagcgtccatgcgcttacaaagccccgt gatgctgcccgggtgaatggcggcttgactgcccggcttgaagggaaggt gtttgtgcgtcttgatctcacgccaccaccaccggattacgtcgggctcg aactcgaagggtccggtaaggggagtggtcgagtgcgccagcttgttgat gacgacattgaccattcggccgttgcgcgtgatctccttcgtctccgaaa caagctcgaagccgtaaggcgccttcccgccgacgtacccgcccaattcg cgctgaaggttcttcgtgtcgagaatcttcgccgacttcagcgaagattc tttgtgcgacgcgtcgagccgcataatcaggtgaatcaggtccatgacgt ttccctgccggaagacgccttcctgagtggaaacaatcgtcacgcccagg gcgagcaattccgagacaatcggaatcgcgtccatgaccttcaggcgcga gaagcgcgacacgtcatagacaatgatcatgttgagccgcccggcgcggc attcgttcaggatgcgttcgaactccgggcgctccgccgtcccgaacgcc gacgtgcccggcgcttcgctgaaatgcccgacgaacctgaaccggccccc gtcgcgctcgacttcgcgctgaaggtcggccgccttgtcttcgttggcgc tacgctgtgtcgctgggcttgctgcgctcgaattttcgcgctcgcgcgac tgacggtcgtaagcacccgcgtacgtgtccatgctagccatggctggcca cacctgcatgcattgcactcttccgccgttgctggcaatggaagtgatgt caaggttttgcttccttgaaacagggaatgaggcagctgacttaaggcca gtgaaaggtgcaaccatgttagcttgagcaacattgctgcgggtggcaac tgctgcggaggaaagaactgaggaagccatatgttctctcctgcaaaatt gcggttttccagaaaatcctctgcaacccgagataaagctgctttgctac tttgagcaaagccgggggcggaactgttttgatcagtgcgaccactagac gacccacttataaggtccgtcgtcagtaacctcttatcggaatttttaag tctccgaccgggaagtctggcaagtgctcatatctccaaagtaccatcca tactcgggtcttgaaacggatatcgcgtttcaactgcaatgagcaggcaa taaatgatgccacaatcgacctgaaagggaggaactggaaagactggacg cacgcggcctgactgtcgaggccatcgtatccctgtgagcattgttggtg acgagcagccggaattactttgatccaacccctccgctgctatagtgcag tcggcttctgacgttcagtgcagccgtcttctgaaaacgacatgtcgcac aagtcctaagttacgcgacaggctgccgccctgcccttttcctggcgttt tcttgtcgcgtgttttagtcgcataaagtagaatacttgcgactagaacc ggagacattacgccatgaacaagagcgccgccgctggcctgctgggctat gcccgcgtcagcaccgacgaccaggacttgaccaaccaacgggccgaact gcacgcggccggctgcaccaagctgttttccgagaagatcaccggcacca ggcgcgaccgcccggagctggccaggatgcttgaccacctacgccctggc gacgttgtgacagtgaccaggctagaccgcctggcccgcagcacccgcga cctactggacattgccgagcgcatccaggaggccggcgcgggcctgcgta gcctggcagagccgtgggccgacaccaccacgccggccggccgcatggtg ttgaccgtgttcgccggcattgccgagttcgagcgttccctaatcatcga ccgcacccggagcgggcgcgaggccgccaaggcccgaggcgtgaagtttg gcccccgccctaccctcaccccggcacagatcgcgcacgcccgcgagctg atcgaccaggaaggccgcaccgtgaaagaggcggctgcactgcttggcgt gcatcgctcgaccctgtaccgcgcacttgagcgcagcgaggaagtgacgc ccaccgaggccaggcggcgcggtgccttccgtgaggacgcattgaccgag gccgacgccctggcggccgccgagaatgaacgccaagaggaacaagcatg aaaccgcaccaggacggccaggacgaaccgtttttcattaccgaagagat cgaggcggagatgatcgcggccgggtacgtgttcgagccgcccgcgcacg tctcaaccgtgcggctgcatgaaatcctggccggtttgtctgatgccaag ctggcggcctggccggccagcttggccgctgaagaaaccgagcgccgccg tctaaaaaggtgatgtgtatttgagtaaaacagcttgcgtcatgcggtcg ctgcgtatatgatgcgatgagtaaataaacaaatacgcaaggggaacgca tgaaggttatcgctgtacttaaccagaaaggcgggtcaggcaagacgacc atcgcaacccatctagcccgcgccctgcaactcgccggggccgatgttct gttagtcgattccgatccccagggcagtgcccgcgattgggcggccgtgc gggaagatcaaccgctaaccgttgtcggcatcgaccgcccgacgattgac cgcgacgtgaaggccatcggccggcgcgacttcgtagtgatcgacggagc gccccaggcggcggacttggctgtgtccgcgatcaaggcagccgacttcg tgctgattccggtgcagccaagcccttacgacatatgggccaccgccgac ctggtggagctggttaagcagcgcattgaggtcacggatggaaggctaca agcggcctttgtcgtgtcgcgggcgatcaaaggcacgcgcatcggcggtg aggttgccgaggcgctggccgggtacgagctgcccattcttgagtcccgt atcacgcagcgcgtgagctacccaggcactgccgccgccggcacaaccgt tcttgaatcagaacccgagggcgacgctgcccgcgaggtccaggcgctgg ccgctgaaattaaatcaaaactcatttgagttaatgaggtaaagagaaaa tgagcaaaagcacaaacacgctaagtgccggccgtccgagcgcacgcagc agcaaggctgcaacgttggccagcctggcagacacgccagccatgaagcg ggtcaactttcagttgccggcggaggatcacaccaagctgaagatgtacg cggtacgccaaggcaagaccattaccgagctgctatctgaatacatcgcg cagctaccagagtaaatgagcaaatgaataaatgagtagatgaattttag cggctaaaggaggcggcatggaaaatcaagaacaaccaggcaccgacgcc gtggaatgccccatgtgtggaggaacgggcggttggccaggcgtaagcgg ctgggttgtctgccggccctgcaatggcactggaacccccaagcccgagg aatcggcgtgagcggtcgcaaaccatccggcccggtacaaatcggcgcgg cgctgggtgatgacctggtggagaagttgaaggccgcgcaggccgcccag cggcaacgcatcgaggcagaagcacgccccggtgaatcgtggcaagcggc cgctgatcgaatccgcaaagaatcccggcaaccgccggcagccggtgcgc cgtcgattaggaagccgcccaagggcgacgagcaaccagattttttcgtt ccgatgctctatgacgtgggcacccgcgatagtcgcagcatcatggacgt ggccgttttccgtctgtcgaagcgtgaccgacgagctggcgaggtgatcc gctacgagcttccagacgggcacgtagaggtttccgcagggccggccggc atggccagtgtgtgggattacgacctggtactgatggcggtttcccatct aaccgaatccatgaaccgataccgggaagggaagggagacaagcccggcc gcgtgttccgtccacacgttgcggacgtactcaagttctgccggcgagcc gatggcggaaagcagaaagacgacctggtagaaacctgcattcggttaaa caccacgcacgttgccatgcagcgtacgaagaaggccaagaacggccgcc tggtgacggtatccgagggtgaagccttgattagccgctacaagatcgta aagagcgaaaccgggcggccggagtacatcgagatcgagctagctgattg gatgtaccgcgagatcacagaaggcaagaacccggacgtgctgacggttc accccgattactttttgatcgatcccggcatcggccgttttctctaccgc ctggcacgccgcgccgcaggcaaggcagaagccagatggttgttcaagac gatctacgaacgcagtggcagcgccggagagttcaagaagttctgtttca ccgtgcgcaagctgatcgggtcaaatgacctgccggagtacgatttgaag gaggaggcggggcaggctggcccgatcctagtcatgcgctaccgcaacct gatcgagggcgaagcatccgccggttcctaatgtacggagcagatgctag ggcaaattgccctagcaggggaaaaaggtcgaaaaggtctctttcctgtg gatagcacgtacattgggaacccaaagccgtacattgggaaccggaaccc gtacattgggaacccaaagccgtacattgggaaccggtcacacatgtaag tgactgatataaaagagaaaaaaggcgatttttccgcctaaaactcttta aaacttattaaaactcttaaaacccgcctggcctgtgcataactgtctgg ccagcgcacagccgaagagctgcaaaaagcgcctacccttcggtcgctgc gctccctacgccccgccgcttcgcgtcggcctatcgcggccgctggccgc tcaaaaatggctggcctacggccaggcaatctaccagggcgcggacaagc cgcgccgtcgccactcgaccgccggcgcccacatcaaggcaccctgcctc gcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccgga gacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtc agggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccag tcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagag cagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatg cgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactg actcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactca aaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaa catgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgt tgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat cgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacca ggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgc cgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctt tctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctc caagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcct tatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcg ccactggcagcagccactggtaacaggattagcagagcgaggtatgtagg cggtgctacagagttcttgaagtggtggcctaactacggctacactagaa ggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaa agagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtgg tttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaag aagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaac tcacgttaagggattttggtcatgcatgatatatctcccaatttgtgtag ggcttattatgcacgcttaaaaataataaaagcagacttgacctgatagt ttggctgtgagcaattatgtgcttagtgcatctaatcgcttgagttaacg ccggcgaagcggcgtcggcttgaacgaatttctagctagacattatttgc cgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaac tgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctg tctagcttcaagtatgacgggctgatactgggccggcaggcgctccattg cccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctg taccaaatgcgggacaacgtaagcactacatttcgctcatcgccagccca gtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaata gatcctgttcaggaaccggatcaaagagttcctccgccgctggacctacc aaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaat gtcgatcgtggctggctcgaagatacctgcaagaatgtcattgcgctgcc attctccaaattgcagttcgcgcttagctggataacgccacggaatgatg tcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctc tccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcg ttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactg tgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccag caacgtcggttcgagatggcgctcgatgacgccaactacctctgatagtt gagtcgatacttcggcgatcaccgcttcccccatgatgtttaactttgtt ttagggcgactgccctgctgcgtaacatcgttgctgctccataacatcaa acatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatag actgtaccccaaaaaaacatgtcataacaagaagccatgaaaaccgccac tgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtg acggcagttacgctacttgcattacagcttacgaaccgaacgaggcttat gtccactgggttcgtgcccgaattgatccagg

>SEQ ID NO 5 pAM305, with dicistronic aadA-gfp1-10 gene, for expression in chloroplasts

GCTCCCCCGCCGTCGTTCAATGAGAATGGATAAGAGGCTCGTGGGATTGA CGTGAGGGGGCAGGGATGGCTATATTTCTGGGAGAATTAACCGATCGACG TGCaAGCGGACATTTATTTTaAATTCGATAATTTTTGCAAAAACATTTCG ACATATTTATTTATTTTATTATTATGAGAATCAATCCTACTACTTCTGGT TCTGGGGTTTCCACGgctactagcGAAGCGGTGATCGCCGAAGTATCGAC TCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGT TGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCA CACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAAC AACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTG GAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGAC GACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGA ATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCG ACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCC TTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGA TCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCG ACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGG TACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTG GGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTA GACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGAT CAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGT gGGCAAAgaaCAAAAACTCATTTCTGAAGAAGACTTGTAACTGCAGATAA CCCAAATAATGTTTTAAAATTTTAAAAATAATGTAGGAGGAAAAATTATG AGCAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATT AGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGAGGAGAGGGTGAAG GTGATGCTACAATCGGAAAACTCACCCTTAAATTTATTTGCACTACTGGA AAACTACCTGTTCCtTGGCCAACACTTGTCACTACTCTGACCTATGGTGT TCAATGCTTTTCCCGTTATCCGGATCACATGAAAAGGCATGACTTTTTCA AGAGTGCCATGCCCGAAGGTTATGTtCAGGAACGCACTATATCTTTCAAA GATGACGGGAAATACAAGACGCGTGCTGTAGTCAAGTTTGAAGGTGATAC CCTTGTTAATCGTATCGAGTTAAAGGGTACTGATTTTAAAGAAGATGGAA ACATTCTCGGACACAAACTtGAGTACAACTTTAACTCACACAATGTATAC ATCACGGCAGACAAACAAAAGAATGGAATCAAAGCTAACTTCACAGTTCG CCACAACGTTGAAGATGGTTCCGTTCAACTAGCAGACCATTATCAACAAA ATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTG TCGACACAAACTGTCCTTTCGAAAGATCCCAACGAAAAGGGTACCTAAGc tctagaGATCCTGGCCTAGTCTATAGGAGGTTTTGAAAAGAAAGGAGCAA TAATCATTTTCTTGTTCTATCAAGAGGGTGCTATTGCTCCTTTCTTTTTT TCTTTTTATTTATTTACTAGTATTTTACTTACATAGACTTTTTTGTTTAC ATTATAGAAAAAGAAGGAGAGGTTATTTTCTTGCATTTATTCATG

>SEQ ID NO 6 - pAM29. Sulfite-reductase-GFP11 fusion protein, in a T7 P/T cassette.

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGG TGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT CCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTAC GGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTT CTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT CGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGG TTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAAT ATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAA CCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG AATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTAT TCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAAT GAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCC CCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACT GAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTC AACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAAC CGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACAC TGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA CCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCA TCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGT CAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTAC CTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAT CGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATA CCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAG ACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTT CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGA GATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACC GCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTA GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAC ATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCG AACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGG GTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTA TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTG AGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCT GTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTC TGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTG GGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGAC GGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCC GGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAG GCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGT CTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAAT GTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTT GGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGA TACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAA CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGAT GCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTA ATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAG ATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTA CGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACG TTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTC TGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAG GAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCT GCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCG AGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGC GCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCA CCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACG ATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCT CAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGA TATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCC GCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGC CATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCA GCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCC CGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCC AGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCG CGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTA CCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGAC ATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGG CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGC GCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTC TACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAA TCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCA ACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTT GGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTT TCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAA GAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATT CACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAA AGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATG CGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCA CCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCC CCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAG CCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGG CGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCG GCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATA GGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAA CTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCAC AGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGCCAGTGAAATGTCCCGG CGAGTACCAAGTTGATGGCAAAAAAGTTATACTAGACGAGGACTGTTTTA TGCAAAACCCAGAGGACTGGGACGAAAAAGTGGCTGAGTGGTTGGCCAGA GAGCTAGAGGGCATACAAAAAATGACCGAGGAGCATTGGAAGTTGGTAAA ATACTTAAGGGAGTATTGGGAGACCTTCGGCTCCTGCCCGCCAATTAAAA TGGTCACTAAAGAGACGGGCTTCTCCTTGGAGAAAATCTACCAGCTATTC CCCTCGGGGCCTGCGCACGGAGCTTGTAAAGTGGCAGGCGCGCCGAAGCC CACAGGCTGCGTCGGATCCGGTGGAGGGTCTGGTGGCGGATCAACTAGTC GTGACCACATGGTCCTTCATGAGTACGTAAATGCTGCTGGGATTACATAA ttctagaTAACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTA ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAA CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCT GAAAGGAGGAACTATATCCGGAT

>SEQ ID NO 7 - pAM30. Pt-VirD2 (without GFP1)1, in a T7 P/T cassette.

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGG TGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT CCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTAC GGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTT CTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT CGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGG TTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAAT ATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAA CCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG AATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTAT TCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAAT GAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCC CCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACT GAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTC AACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAAC CGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACAC TGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA CCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCA TCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGT CAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTAC CTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAT CGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATA CCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAG ACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTT CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGA GATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACC GCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTA GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAC ATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCG AACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGG GTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTA TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTG AGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCT GTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTC TGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTG GGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGAC GGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCC GGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAG GCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGT CTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAAT GTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTT GGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGA TACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAA CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGAT GCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTA ATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAG ATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTA CGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACG TTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTC TGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAG GAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCT GCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCG AGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGC GCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCA CCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACG ATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCT CAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGA TATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCC GCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGC CATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCA GCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCC CGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCC AGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCG CGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTA CCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGAC ATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGG CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGC GCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTC TACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAA TCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCA ACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTT GGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTT TCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAA GAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATT CACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAA AGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATG CGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCA CCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCC CCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAG CCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGG CGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCG GCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATA GGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAA CTTTAAGAAGGAGATATACCATGGGCATGCAGGTGTGGCCAGCGATGGCT CCCGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAA GACCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAA AGCTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCG GATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTA TGACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACA TTATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCA AGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCG CTATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTAC ATGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATA TCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGC AGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAG AAAGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTT GAGCGGATGCTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCAT TAAAACGGCTCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGAC CTCGATCAACGCGCGGTCTATGATTCTAGATATACTCGAGCACCACCACC ACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTG GCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAA ACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

>SEQ ID NO 8 pAM31

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGG TGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT CCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTAC GGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTT CTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT CGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGG TTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAAT ATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAA CCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG AATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTAT TCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAAT GAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCC CCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACT GAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTC AACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAAC CGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACAC TGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA CCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCA TCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGT CAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTAC CTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAT CGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATA CCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAG ACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTT CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGA GATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACC GCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTA GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAC ATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCG AACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGG GTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTA TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTG AGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCT GTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTC TGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTG GGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGAC GGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCC GGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAG GCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGT CTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAAT GTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTT GGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGA TACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAA CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGAT GCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTA ATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAG ATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTA CGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACG TTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTC TGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAG GAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCT GCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCG AGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGC GCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCA CCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACG ATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCT CAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGA TATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCC GCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGC CATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCA GCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCC CGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCC AGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCG CGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTA CCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGAC ATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGG CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGC GCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTC TACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAA TCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCA ACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTT GGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTT TCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAA GAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATT CACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAA AGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATG CGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCA CCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCC CCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAG CCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGG CGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCG GCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATA GGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAA CTTTAAGAAGGAGATATACCATGGGCATGCAGGTGTGGCCAGCGCGTGAC CACATGGTCCTTCATGAGTACGTAAATGCTGCTGGGATTACAATGGCTCC CGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAAGA CCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAAAG CTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCGGA TCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTATG ACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACATT ATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCAAG CCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCGCT ATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTACAT GTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATATC CAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGCAG AGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAGAA AGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTTGA GCGGATGCTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCATTA AAACGGCTCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGACCT CGATCAACGCGCGGTCTATGATTCTAGATATACTCGAGCACCACCACCAC CACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGC TGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAAC GGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

>SEQ ID NO 9 pAM32

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGG TGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT CCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTAC GGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTT CTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT CGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGG TTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAAT ATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAA CCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG AATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTAT TCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAAT GAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCC CCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACT GAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTC AACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAAC CGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACAC TGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA CCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCA TCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGT CAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTAC CTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAT CGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATA CCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAG ACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTT CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGA GATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACC GCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTA GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAC ATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCG AACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGG GTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTA TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTG AGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCT GTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTC TGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTG GGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGAC GGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCC GGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAG GCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGT CTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAAT GTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTT GGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGA TACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAA CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGAT GCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTA ATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAG ATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTA CGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACG TTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTC TGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAG GAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCT GCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCG AGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGC GCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCA CCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACG ATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCT CAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGA TATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCC GCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGC CATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCA GCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCC CGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCC AGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCG CGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTA CCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGAC ATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGG CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGC GCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTC TACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAA TCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCA ACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTT GGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTT TCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAA GAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATT CACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAA AGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATG CGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCA CCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCC CCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAG CCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGG CGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCG GCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATA GGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAA CTTTAAGAAGGAGATATACCATGGGCATGCAGGTGTGGCCAGCGCGTGAC CACATGGTCCTTCATGAGTACGTAAATGCTGCTGGGATTACAGGTGGAGG GTCTGGTGGCGGATCAATGGCTCCCGATCGGGCCCAAGTAATCATTCGCA TTGTGCCAGGAGGTGGAACCAAGACCCTTCAGCAGATAATCAATCAGCTG GAGTACCTGTCCCGAAAGGGAAAGCTGGAACTCCAGCGTTCAGCCCGGCA TCTCGATATTCCCGTTCCGCCGGATCAAATCCGTGAGCTTGCCCAAAGCT GGGTTACGGAGGCCGGGATTTATGACGAAAGTCAGTCAGACGATGACAGG CAACAAGACTTAACAACACACATTATTGTGAGCTTCCCCGCAGGTACCGA CCAAACCGCAGCTTATGAAGCAAGCCGGGAATGGGCAGCCGAGATGTTTG GGTCAGGATACGGGGGTGGCCGCTATAACTATCTGACAGCCTACCACGTG GACCGCGATCATCCACATTTACATGTCGTGGTCAATCGTCGGGAACTTCT GGGGCAGGGGTGGCTGAAAATATCCAGGCGCCATCCCCAGCTGAATTATG ACGGCTTACGGAAAAAGATGGCAGAGATTTCACTTCGTCACGGCATAGTC CTGGATGCGACTTCCCGAGCAGAAAGGGGAATAGCAGAGCGACCAATCAC CTATGCTGAATATCGACGTCTTGAGCGGATGCTCGAAGTTATGGCAGAAG TTCGGCCCATAGCCCGATCCATTAAAACGGCTCACGACGATGCGCGAGCG GAATTAATGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATGATTCTA GATATACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAA AGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG CATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA GGAGGAACTATATCCGGAT

>SEQ ID NO 10 pAM33

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGG TGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT CCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTAC GGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTT CTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT CGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGG TTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAAT ATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAA CCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG AATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTAT TCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAAT GAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCC CCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACT GAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTC AACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAAC CGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACAC TGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA CCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCA TCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGT CAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTAC CTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAT CGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATA CCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAG ACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTT CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGA GATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACC GCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTA GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAC ATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCG AACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGG GTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTA TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTG AGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCT GTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTC TGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTG GGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGAC GGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCC GGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAG GCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGT CTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAAT GTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTT GGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGA TACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAA CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGAT GCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTA ATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAG ATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTA CGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACG TTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTC TGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAG GAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCT GCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCG AGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGC GCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCA CCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACG ATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCT CAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGA TATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCC GCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGC CATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCA GCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCC CGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCC AGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCG CGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTA CCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGAC ATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGG CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGC GCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTC TACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAA TCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCA ACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTT GGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTT TCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAA GAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATT CACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAA AGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATG CGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCA CCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCC CGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGC CCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGC GCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGG CGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAG GGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAAC TTTAAGAAGGAGATATACCATGGGCATGCAGGTGTGGCCAGCGATGGCTC CCGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAAG ACCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAAA GCTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCGG ATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTAT GACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACAT TATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCAA GCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCGC TATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTACA TGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATAT CCAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGCA GAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAGA AAGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTTG AGCGGATGCGTGACCACATGGTCCTTCATGAGTACGTAAATGCTGCTGGG ATTACACTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCATTAA AACGGCTCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGACCTC GATCAACGCGCGGTCTATGATTCTAGATATACTCGAGCACCACCACCACC ACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCT GCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACG GGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

>SEQ ID NO 11 pAM34

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGG TGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT CCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTAC GGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTT CTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT CGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGG TTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAAT ATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAA CCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG AATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTAT TCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAAT GAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCC CCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACT GAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTC AACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAAC CGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACAC TGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA CCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCA TCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGT CAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTAC CTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAT CGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATA CCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAG ACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTT CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGA GATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACC GCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTA GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAC ATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCG AACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGG GTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTA TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTG AGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCT GTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTC TGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTG GGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGAC GGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCC GGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAG GCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGT CTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAAT GTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTT GGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGA TACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAA CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGAT GCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTA ATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAG ATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTA CGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACG TTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTC TGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAG GAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCT GCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCG AGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGC GCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCA CCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACG ATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCT CAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGA TATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCC GCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGC CATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCA GCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCC CGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCC AGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCG CGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTA CCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGAC ATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGG CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGC GCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTC TACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAA TCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCA ACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTT GGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTT TCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAA GAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATT CACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAA AGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATG CGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCA CCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCC CCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAG CCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGG CGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCG GCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATA GGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAA CTTTAAGAAGGAGATATACCATGGGCATGCAGGTGTGGCCAGCGATGGCT CCCGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAA GACCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAA AGCTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCG GATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTA TGACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACA TTATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCA AGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCG CTATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTAC ATGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATA TCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGC AGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAG AAAGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTT GAGCGGATGGGTGGAGGGTCTGGTGGCGGATCACGTGACCACATGGTCCT TCATGAGTACGTAAATGCTGCTGGGATTACACTCGAAGTTATGGCAGAAG TTCGGCCCATAGCCCGATCCATTAAAACGGCTCACGACGATGCGCGAGCG GAATTAATGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATGATTCTA GATATACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAA AGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG CATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA GGAGGAACTATATCCGGAT

>SEQ ID NO 12 pAM35

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGG TGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT CCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTAC GGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTT CTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT CGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGG TTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAAT ATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAA CCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG AATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTAT TCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAAT GAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCC CCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACT GAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTC AACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAAC CGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACAC TGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA CCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCA TCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGT CAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTAC CTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAT CGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATA CCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAG ACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTT CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGA GATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACC GCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTA GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAC ATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCG AACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGG GTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTA TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTG AGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCT GTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTC TGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTG GGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGAC GGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCC GGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAG GCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGT CTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAAT GTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTT GGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGA TACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAA CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGAT GCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTA ATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAG ATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTA CGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACG TTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTC TGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAG GAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCT GCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCG AGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGC GCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCA CCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACG ATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCT CAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGA TATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCC GCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGC CATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCA GCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCC CGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCC AGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCG CGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTA CCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGAC ATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGG CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGC GCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTC TACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAA TCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCA ACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTT GGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTT TCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAA GAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATT CACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAA AGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATG CGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCA CCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCC CCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAG CCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGG CGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCG GCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATA GGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAA CTTTAAGAAGGAGATATACCATGGGCATGCAGGTGTGGCCAGCGATGGCT CCCGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAA GACCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAA AGCTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCG GATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTA TGACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACA TTATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCA AGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCG CTATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTAC ATGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATA TCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGC AGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAG AAAGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTT GAGCGGATGCGTGACCACATGGTCCTTCATGAGTACGTAAATGCTGCTGG GATTACAGGTGGAGGGTCTGGTGGCGGATCACTCGAAGTTATGGCAGAAG TTCGGCCCATAGCCCGATCCATTAAAACGGCTCACGACGATGCGCGAGCG GAATTAATGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATGATTCTA GATATACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAA AGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG CATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA GGAGGAACTATATCCGGAT

>SEQ ID NO 13 pAM36

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGG TGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCT CCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTAC GGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGG CCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTT CTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCT CGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGG TTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAAT ATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAA CCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG AATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTAT TCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAAT GAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTA TCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCC CCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACT GAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTC AACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAAC CGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTG TTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACAC TGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATA CCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCA TCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGT CAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTAC CTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAAT CGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATA CCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAG ACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATG TAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTT CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGA GATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACC GCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTC CGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTA GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTAC ATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCG CAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCG AACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCG CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGG GTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTA TCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCG GCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTT TCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGT GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTG AGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCT GTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTC TGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTG GGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGAC GGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCC GGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAG GCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGT CTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAAT GTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTT GGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGA TACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAA CATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGAT GCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTA ATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAG ATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTA CGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACG TTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTC TGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAG GAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCT GCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCG AGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGC GCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCA CCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACG ATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCT CAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACAT TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT TGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGA TTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGA TATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCC GCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGC CATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCA GCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCC CGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCC AGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCG CGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTA CCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGAC ATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGG CATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGC GCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTC TACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAA TCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCA ACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTT GGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTT TCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAA GAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATT CACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAA AGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATG CGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCA CCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCC CCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAG CCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGG CGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCG GCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATA GGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAA CTTTAAGAAGGAGATATACCATGGGCATGCAGGTGTGGCCAGCGATGGCT CCCGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAA GACCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAA AGCTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCG GATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTA TGACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACA TTATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCA AGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCG CTATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTAC ATGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATA TCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGC AGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAG AAAGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTT GAGCGGATGGGTGGAGGGTCTCGTGACCACATGGTCCTTCATGAGTACGT AAATGCTGCTGGGATTACAGGTGGCGGATCACTCGAAGTTATGGCAGAAG TTCGGCCCATAGCCCGATCCATTAAAACGGCTCACGACGATGCGCGAGCG GAATTAATGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATGATTCTA GATATACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAA AGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG CATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAA GGAGGAACTATATCCGGAT

>SEQ ID NO 14 pAM407

ccgggctggttgccctcgccgctgggctggcggccgtctatggccctgca aacgcgccagaaacgccgtcgaagccgtgtgcgagacaccgcggccgccg gcgttgtggatacctcgcggaaaacttggccctcactgacagatgagggg cggacgttgacacttgaggggccgactcacccggcgcggcgttgacagat gaggggcaggctcgatttcggccggcgacgtggagctggccagcctcgca aatcggcgaaaacgcctgattttacgcgagtttcccacagatgatgtgga caagcctggggataagtgcctgcggtattgacacttgaggggcgcgacta ctgacagatgaggggcgcgatccttgacacttgaggggcagagtgctgac agatgaggggcgcacctattgacatttgaggggctgtccacaggcagaaa atccagcatttgcaagggtttccgcccgtttttcggccaccgctaacctg tcttttaacctgcttttaaaccaatatttataaaccttgtttttaaccag ggctgcgccctgtgcgcgtgaccgcgcacgccgaaggggggtgccccccc ttctcgaaccctcccggcccgctaacgcgggcctcccatccccccagggg ctgcgcccctcggccgcgaacggcctcaccccaaaaatggcagcgctggc agtccttgccattgccgggatcggggcagtaacgggatgggcgatcagcc cgagcgcgacgcccggaagcattgacgtgccgcaggtgctggcatcgaca ttcagcgaccaggtgccgggcagtgagggcggcggcctgggtggcggcct gcccttcacttcggccgtcggggcattcacggacttcatggcggggccgg caatttttaccttgggcattcttggcatagtggtcgcgggtgccgtgctc gtgttcgggggtgcgataaacccagcgaaccatttgaggtgataggtaag attataccgaggtatgaaaacgagaattggacctttacagaattactcta tgaagcgccatatttaaaaagctaccaagacgaagaggatgaagaggatg aggaggcagattgccttgaatatattgacaatactgataagataatatat cttttatatagaagatatcgccgtatgtaaggatttcagggggcaaggca taggcagcgcgcttatcaatatatctatagaatgggcaaagcataaaaac ttgcatggactaatgcttgaaacccaggacaataaccttatagcttgtaa attctatcataattgggtaatgactccaacttattgatagtgttttatgt tcagataatgcccgatgactttgtcatgcagctccaccgattttgagaac gacagcgacttccgtcccagccgtgccaggtgctgcctcagattcaggtt atgccgctcaattcgctgcgtatatcgcttgctgattacgtgcagctttc ccttcaggcgggattcatacagcggccagccatccgtcatccatatcacc acgtcaaagggtgacagcaggctcataagacgccccagcgtcgccatagt gcgttcaccgaatacgtgcgcaacaaccgtcttccggagactgtcatacg cgtaaaacagccagcgctggcgcgatttagccccgacatagccccactgt tcgtccatttccgcgcagacgatgacgtcactgcccggctgtatgcgcga ggttaccgactgcggcctgagttttttaagtgacgtaaaatcgtgttgag gccaacgcccataatgcgggctgttgcccggcatccaacgccattcatgg ccatatcaatgattttctggtgcgtaccgggttgagaagcggtgtaagtg aactgcagttgccatgttttacggcagtgagagcagagatagcgctgatg tccggcggtgcttttgccgttacgcaccaccccgtcagtagctgaacagg agggacagctgatagacacagaagccactggagcacctcaaaaacaccat catacactaaatcagtaagttggcagcatcacccataattgtggtttcaa aatcggctccgtcgatactatgttatacgccaactttgaaaacaactttg aaaaagctgttttctggtatttaaggttttagaatgcaaggaacagtgaa ttggagttcgtcttgttataattagcttcttggggtatctttaaatactg tagaaaagaggaaggaaataataaatggctaaaatgagaatatcaccgga attgaaaaaactgatcgaaaaataccgctgcgtaaaagatacggaaggaa tgtctcctgctaaggtatataagctggtgggagaaaatgaaaacctatat ttaaaaatgacggacagccggtataaagggaccacctatgatgtggaacg ggaaaaggacatgatgctatggctggaaggaaagctgcctgttccaaagg tcctgcactttgaacggcatgatggctggagcaatctgctcatgagtgag gccgatggcgtcctttgctcggaagagtatgaagatgaacaaagccctga aaagattatcgagctgtatgcggagtgcatcaggctctttcactccatcg acatatcggattgtccctatacgaatagcttagacagccgcttagccgaa ttggattacttactgaataacgatctggccgatgtggattgcgaaaactg ggaagaagacactccatttaaagatccgcgcgagctgtatgattttttaa agacggaaaagcccgaagaggaacttgtcttttcccacggcgacctggga gacagcaacatctttgtgaaagatggcaaagtaagtggctttattgatct tgggagaagcggcagggcggacaagtggtatgacattgccttctgcgtcc ggtcgatcagggaggatatcggggaagaacagtatgtcgagctatttttt gacttactggggatcaagcctgattgggagaaaataaaatattatatttt actggatgaattgttttagtacctagatgtggcgcaacgatgccggcgac aagcaggagcgcaccgacttcttccgcatcaagtgttttggctctcaggc cgaggcccacggcaagtatttgggcaaggggtcgctggtattcgtgcagg gcaagattcggaataccaagtacgagaaggacggccagacggtctacggg accgacttcattgccgataaggtggattatctggacaccaaggcaccagg cgggtcaaatcaggaataagggcacattgccccggcgtgagtcggggcaa tcccgcaaggagggtgaatgaatcggacgtttgaccggaaggcatacagg caagaactgatcgacgcggggttttccgccgaggatgccgaaaccatcgc aagccgcaccgtcatgcgtgcgccccgcgaaaccttccagtccgtcggct cgatggtccagcaagctacggccaagatcgagcgcgacagcgtgcaactg gctccccctgccctgcccgcgccatcggccgccgtggagcgttcgcgtcg tctcgaacaggaggcggcaggtttggcgaagtcgatgaccatcgacacgc gaggaactatgacgaccaagaagcgaaaaaccgccggcgaggacctggca aaacaggtcagcgaggccaagcaggccgcgttgctgaaacacacgaagca gcagatcaaggaaatgcagctttccttgttcgatattgcgccgtggccgg acacgatgcgagcgatgccaaacgacacggcccgctctgccctgttcacc acgcgcaacaagaaaatcccgcgcgaggcgctgcaaaacaaggtcatttt ccacgtcaacaaggacgtgaagatcacctacaccggcgtcgagctgcggg ccgacgatgacgaactggtgtggcagcaggtgttggagtacgcgaagcgc acccctatcggcgagccgatcaccttcacgttctacgagctttgccagga cctgggctggtcgatcaatggccggtattacacgaaggccgaggaatgcc tgtcgcgcctacaggcgacggcgatgggcttcacgtccgaccgcgttggg cacctggaatcggtgtcgctgctgcaccgcttccgcgtcctggaccgtgg caagaaaacgtcccgttgccaggtcctgatcgacgaggaaatcgtcgtgc tgtttgctggcgaccactacacgaaattcatatgggagaagtaccgcaag ctgtcgccgacggcccgacggatgttcgactatttcagctcgcaccggga gccgtacccgctcaagctggaaaccttccgcctcatgtgcggatcggatt ccacccgcgtgaagaagtggcgcgagcaggtcggcgaagcctgcgaagag ttgcgaggcagcggcctggtggaacacgcctgggtcaatgatgacctggt gcattgcaaacgctagggccttgtggggtcagttccggctgggggttcag cagccagcgctttactggcatttcaggaacaagcgggcactgctcgacgc acttgcttcgctcagtatcgctcgggacgcacggcgcgctctacgaactg ccgataaacagaggattaaaattgacaattgtgattaaggctcagattcg acggcttggagcggccgacgtgcaggatttccgcgagatccgattgtcgg ccctgaagaaagctccagagatgttcgggtccgtttacgagcacgaggag aaaaagcccatggaggcgttcgctgaacggttgcgagatgccgtggcatt cggcgcctacatcgacggcgagatcattgggctgtcggtcttcaaacagg aggacggccccaaggacgctcacaaggcgcatctgtccggcgttttcgtg gagcccgaacagcgaggccgaggggtcgccggtatgctgctgcgggcgtt gccggcgggtttattgctcgtgatgatcgtccgacagattccaacgggaa tctggtggatgcgcatcttcatcctcggcgcacttaatatttcgctattc tggagcttgttgtttatttcggtctaccgcctgccgggcggggtcgcggc gacggtaggcgctgtgcagccgctgatggtcgtgttcatctctgccgctc tgctaggtagcccgatacgattgatggcggtcctgggggctatttgcgga actgcgggcgtggcgctgttggtgttgacaccaaacgcagcgctagatcc tgtcggcgtcgcagcgggcctggcgggggcggtttccatggcgttcggaa ccgtgctgacccgcaagtggcaacctcccgtgcctctgctcacctttacc gcctggcaactggcggccggaggacttctgctcgttccagtagctttagt gtttgatccgccaatcccgatgcctacaggaaccaatgttctcggcctgg cgtggctcggcctgatcggagcgggtttaacctacttcctttggttccgg gggatctcgcgactcgaacctacagttgtttccttactgggctttctcag ccccagatctggggtcgatcagccggggatgcatcaggccgacagtcgga acttcgggtccccgacctgtaccattcggtgagcaatggataggggagtt gatatcgtcaacgttcacttctaaagaaatagcgccactcagcttcctca gcggctttatccagcgatttcctattatgtcggcatagttctcaagatcg acagcctgtcacggttaagcgagaaatgaataagaaggctgataattcgg atctctgcgagggagatgatatttgatcacaggcagcaacgctctgtcat cgttacaatcaacatgctaccctccgcgagatcatccgtgtttcaaaccc ggcagcttagttgccgttcttccgaatagcatcggtaacatgagcaaagt ctgccgccttacaacggctctcccgctgacgccgtcccggactgatgggc tgcctgtatcgagtggtgattttgtgccgagctgccggtcggggagctgt tggctggctggtggcaggatatattgtggtgtaaacaaattgacgcttag acaacttaataacacattgcggacgtttttaatgtactggggtggttttt cttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggcc ctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaa aatcctgtttgatggtggttccgaaatcggcaaaatcccttataaatcaa aagaatagcccgagatagggttgagtgttgttccagtttggaacaagagt ccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtcta tcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgg ggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccga tttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaa gaaagcgaaaggagcgggcgccattcaggctgcgcaactgttgggaaggg cgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatg tgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgac gttgtaaaacgacggccagtgAATTCTTAATtaagccaacatggtggagc acgacactctcgtctactccaagaatatcaaagatacagtctcagaagac caaagggctattgagacttttcaacaaagggtaatatcgggaaacctcct cggattccattgcccagctatctgtcacttcatcaaaaggacagtagaaa aggaaggtggcacctacaaatgccatcattgcgataaaggaaaggctatc gttcaagatgcctctgccgacagtggtcccaaagatggacccccacccac gaggagcatcgtggaaaaagaagacgttccaaccacgtcttcaaagcaag tggattgatgtgaacatggtggagcacgacactctcgtctactccaagaa tatcaaagatacagtctcagaagaccaaagggctattgagacttttcaac aaagggtaatatcgggaaacctcctcggattccattgcccagctatctgt cacttcatcaaaaggacagtagaaaaggaaggtggcacctacaaatgcca tcattgcgataaaggaaaggctatcgttcaagatgcctctgccgacagtg gtcccaaagatggacccccacccacgaggagcatcgtggaaaaagaagac gttccaaccacgtcttcaaagcaagtggattgatgtgatatctccactga cgtaagggatgacgcacaatcccactatccttcgcaagacccttcctcta tataaggaagttcatttcatttggagaggacacgctgaaatcaccagtct ctctctacaaatctatctctcTCGAGgtctcGGTACCATGGCTTCCTCAG TTCTTTCCTCCGCAGCAGTTGCCACCCGCAGCAATGTTGCTCAAGCTAAC ATGGTTGCACCTTTCACTGGCCTTAAGTCAGCTGCCTCATTCCCTGTTTC AAGGAAGCAAAACCTTGACATCACTTCCATTGCCAGCAACGGCGGAAGAG TGCAATGCATGCAGGTGTGGCCAATGAGCAAAGGAGAAGAACTTTTCACT GGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAA ATTTTCTGTCAGAGGAGAGGGTGAAGGTGATGCTACAATCGGAAAACTCA CCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCTTGGCCAACA CTTGTCACTACTCTGACCTATGGTGTTCAATGCTTTTCCCGTTATCCGGA TCACATGAAAAGGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATG TTCAGGAACGCACTATATCTTTCAAAGATGACGGGAAATACAAGACGCGT GCTGTAGTCAAGTTTGAAGGTGATACCCTTGTTAATCGTATCGAGTTAAA GGGTACTGATTTTAAAGAAGATGGAAACATTCTCGGACACAAACTTGAGT ACAACTTTAACTCACACAATGTATACATCACGGCAGACAAACAAAAGAAT GGAATCAAAGCTAACTTCACAGTTCGCCACAACGTTGAAGATGGTTCCGT TCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTG TCCTTTTACCAGACAACCATTACCTGTCGACACAAACTGTCCTTTCGAAA GATCCCAACGAAAAGGGAACCTAATTCTAGACTGCTTTAATGAGATATGC GAGACGCCTATGATCGCATGATATTTGCTTTCAATTCTGTTGTGCACGTT GTAAAAAACCTGAGCATGTGTAGCTCAGATCCTTACCGCCGGTTTCGGTT CATTCTAATGAATATATCACCCGTTACTATCGTATTTTTATGAATAATAT TCTCCGTTCAATTTACTGATTGTCCAagcttggcgtaatcatggtcatag ctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacg agccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaac tcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctg tcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggttt gcgtattgggccaaagacaaaagggcgacattcaaccgattgagggaggg aaggtaaatattgacggaaattattcattaaaggtgaattatcaccgtca ccgacttgagccatttgggaattagagccagcaaaatcaccagtagcacc attaccattagcaaggccggaaacgtcaccaatgaaaccatcgatagcag caccgtaatcagtagcgacagaatcaagtttgcctttagcgtcagactgt agcgcgttttcatcggcattttcggtcatagcccccttattagcgtttgc catcttttcataatcaaaatcaccggaaccagagccaccaccggaaccgc ctccctcagagccgccaccctcagaaccgccaccctcagagccaccaccc tcagagccgccaccagaaccaccaccagagccgccgccagcattgacagg aggcccgatctagtaacatagatgacaccgcgcgcgataatttatcctag tttgcgcgctatattttgttttctatcgcgtattaaatgtataattgcgg gactctaatcataaaaacccatctcataaataacgtcatgcattacatgt taattattacatgcttaacgtaattcaacagaaattatatgataatcatc gcaagaccggcaacaggattcaatcttaagaaactttattgccaaatgtt tgaacgatcggggatcatccgggtctgtggcgggaactccacgaaaatat ccgaacgcagcaagatatcgcggtgcatctcggtcttgcctgggcagtcg ccgccgacgccgttgatgtggacgccgggcccgatcatattgtcgctcag gatcgtggcgttgtgcttgtcggccgttgctgtcgtaatgatatcggcac cttcgaccgcctgttccgcagagatcccgtgggcgaagaactccagcatg agatccccgcgctggaggatcatccagccggcgtcccggaaaacgattcc gaagcccaacctttcatagaaggcggcggtggaatcgaaatctcgtgatg gcaggttgggcgtcgcttggtcggtcatttcgaaccccagagtcccgctc agaagaactcgtcaagaaggcgatagaaggcgatgcgctgcgaatcggga gcggcgataccgtaaagcacgaggaagcggtcagcccattcgccgccaag ctcttcagcaatatcacgggtagccaacgctatgtcctgatagcggtccg ccacacccagccggccacagtcgatgaatccagaaaagcggccattttcc accatgatattcggcaagcaggcatcgccatgggtcacgacgagatcatc gccgtcgggcatgcgcgccttgagcctggcgaacagttcggctggcgcga gcccctgatgctcttcgtccagatcatcctgatcgacaagaccggcttcc atccgagtacgtgctcgctcgatgcgatgtttcgcttggtggtcgaatgg gcaggtagccggatcaagcgtatgcagccgccgcattgcatcagccatga tggatactttctcggcaggagcaaggtgagatgacaggagatcctgcccc ggcacttcgcccaatagcagccagtcccttcccgcttcagtgacaacgtc gagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcg ctgcctcgtcctgcagttcattcagggcaccggacaggtcggtcttgaca aaaagaaccgggcgcccctgcgctgacagccggaacacggcggcatcaga gcagccgattgtctgttgtgcccagtcatagccgaatagcctctccaccc aagcggccggagaacctgcgtgcaatccatcttgttcaatcatgcgaaac gatccagatccggtgcagattatttggattgagagtgaatatgagactct aattggataccgaggggaatttatggaacgtcagtggagcatttttgaca agaaatatttgctagctgatagtgaccttaggcgacttttgaacgcgcaa taatggtttctgacgtatgtgcttagctcattaaactccagaaacccgcg gctgagtggctccttcaacgttgcggttctgtcagttccaaacgtaaaac ggcttgtcccgcgtcatcggcgggggtcataacgtgactcccttaattct ccgctcatgatcagattgtcgtttcccgccttcagtttaaactatcagtg tttgacaggatatattggcgggtaaacctaagagaaaagagcgtttatta gaataatcggatatttaaaagggcgtgaaaaggtttatccgttcgtccat ttgtatgtgcatgccaaccacagggttccccagatctggcgccggccagc gagacgagcaagattggccgccgcccgaaacgatccgacagcgcgcccag cacaggtgcgcaggcaaattgcaccaacgcatacagcgccagcagaatgc catagtgggcggtgacgtcgttcgagtgaaccagatcgcgcaggaggccc ggcagcaccggcataatcaggccgatgccgacagcgtcgagcgcgacagt gctcagaattacgatcaggggtatgttgggtttcacgtctggcctccgga ccagcctccgctggtccgattgaacgcgcggattctttatcactgataag ttggtggacatattatgtttatcagtgataaagtgtcaagcatgacaaag ttgcagccgaatacagtgatccgtgccgccctggacctgttgaacgaggt cggcgtagacggtctgacgacacgcaaactggcggaacggttgggggttc agcagccggcgctttactggcacttcaggaacaagcgggcgctgctcgac gcactggccgaagccatgctggcggagaatcatacgcattcggtgccgag agccgacgacgactggcgctcatttctgatcgggaatgcccgcagcttca ggcaggcgctgctcgcctaccgcgatggcgcgcgcatccatgccggcacg cgaccgggcgcaccgcagatggaaacggccgacgcgcagcttcgcttcct ctgcgaggcgggtttttcggccggggacgccgtcaatgcgctgatgacaa tcagctacttcactgttggggccgtgcttgaggagcaggccggcgacagc gatgccggcgagcgcggcggcaccgttgaacaggctccgctctcgccgct gttgcgggccgcgatagacgccttcgacgaagccggtccggacgcagcgt tcgagcagggactcgcggtgattgtcgatggattggcgaaaaggaggctc gttgtcaggaacgttgaaggaccgagaaagggtgacgattgatcaggacc gctgccggagcgcaacccactcactacagcagagccatgtagacaacatc ccctccccctttccaccgcgtcagacgcccgtagcagcccgctacgggct ttttcatgccctgccctagcgtccaagcctcacggccgcgctcggcctct ctggcggccttctggcgctcttccgcttcctcgctcactgactcgctgcg ctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaa tacggttatccacagaatcaggggataacgcaggaaagaacatgtgagca aaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtt tttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaa gtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccc cctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgg atacctgtccgcctttctcccttcgggaagcgtggcgcttttccgctgca taaccctgcttcggggtcattatagcgattttttcggtatatccatcctt tttcgcacgatatacaggattttgccaaagggttcgtgtagactttcctt ggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccaccc gcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgct caacgggaatcctgctctgcgaggctggccggctaccgccggcgtaacag atgagggcaagcggatggctgatgaaaccaagccaaccaggaagggcagc ccacctatcaaggtgtactgccttccagacgaacgaagagcgattgagga aaaggcggcggcggccggcatgagcctgtcggcctacctgctggccgtcg gccagggctacaaaatcacgggcgtcgtggactatgagcacgtccgcgag ctggcccgcatcaatggcgacctgggccgcctgggcggcctgctgaaact ctggctcaccgacgacccgcgcacggcgcggttcggtgatgccacgatcc tcgccctgctggcgaagatcgaagagaagcaggacgagcttggcaaggtc atgatgggcgtggtccgcccgagggcagagccatgacttttttagccgct aaaacggccggggggtgcgcgtgattgccaagcacgtccccatgcgctcc atcaagaagagcgacttcgcggagctggtgaagtacatcaccgacgagca aggcaagaccgagcgcctttgcgacgctca

>SEQ ID NO 15 pJF7

AGTACTTTGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACG TTCAGTGCAGCCGTCTTCTGAAAACGACATGTCGCACAAGTCCTAAGTTA CGCGACAGGCTGCCGCCCTGCCCTTTTCCTGGCGTTTTCTTGTCGCGTGT TTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTACGC CATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCA CCGACGACCAGGACTTGACCAACCAACGGGCCGAACTGCACGCGGCCGGC TGCACCAAGCTGTTTTCCGAGAAGATCACCGGCACCAGGCGCGACCGCCC GGAGCTGGCCAGGATGCTTGACCACCTACGCCCTGGCGACGTTGTGACAG TGACCAGGCTAGACCGCCTGGCCCGCAGCACCCGCGACCTACTGGACATT GCCGAGCGCATCCAGGAGGCCGGCGCGGGCCTGCGTAGCCTGGCAGAGCC GTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGTGTTCG CCGGCATTGCCGAGTTCGAGCGTTCCCTAATCATCGACCGCACCCGGAGC GGGCGCGAGGCCGCCAAGGCCCGAGGCGTGAAGTTTGGCCCCCGCCCTAC CCTCACCCCGGCACAGATCGCGCACGCCCGCGAGCTGATCGACCAGGAAG GCCGCACCGTGAAAGAGGCGGCTGCACTGCTTGGCGTGCATCGCTCGACC CTGTACCGCGCACTTGAGCGCAGCGAGGAAGTGACGCCCACCGAGGCCAG GCGGCGCGGTGCCTTCCGTGAGGACGCATTGACCGAGGCCGACGCCCTGG CGGCCGCCGAGAATGAACGCCAAGAGGAACAAGCATGAAACCGCACCAGG ACGGCCAGGACGAACCGTTTTTCATTACCGAAGAGATCGAGGCGGAGATG ATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGTGCG GCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGC CGGCCAGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGA TGTGTATTTGAGTAAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGAT GCGATGAGTAAATAAACAAATACGCAAGGGGAACGCATGAAGGTTATCGC TGTACTTAACCAGAAAGGCGGGTCAGGCAAGACGACCATCGCAACCCATC TAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCTGTTAGTCGATTCC GATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGATCAACC GCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGG CCATCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCG GACTTGGCTGTGTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGT GCAGCCAAGCCCTTACGACATATGGGCCACCGCCGACCTGGTGGAGCTGG TTAAGCAGCGCATTGAGGTCACGGATGGAAGGCTACAAGCGGCCTTTGTC GTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAGGC GCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACGCAGCGCG TGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAA CCCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAA ATCAAAACTCATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCAC AAACACGCTAAGTGCCGGCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAA CGTTGGCCAGCCTGGCAGACACGCCAGCCATGAAGCGGGTCAACTTTCAG TTGCCGGCGGAGGATCACACCAAGCTGAAGATGTACGCGGTACGCCAAGG CAAGACCATTACCGAGCTGCTATCTGAATACATCGCGCAGCTACCAGAGT AAATGAGCAAATGAATAAATGAGTAGATGAATTTTAGCGGCTAAAGGAGG CGGCATGGAAAATCAAGAACAACCAGGCACCGACGCCGTGGAATGCCCCA TGTGTGGAGGAACGGGCGGTTGGCCAGGCGTAAGCGGCTGGGTTGTCTGC CGGCCCTGCAATGGCACTGGAACCCCCAAGCCCGAGGAATCGGCGTGAGC GGTCGCAAACCATCCGGCCCGGTACAAATCGGCGCGGCGCTGGGTGATGA CCTGGTGGAGAAGTTGAAGGCCGCGCAGGCCGCCCAGCGGCAACGCATCG AGGCAGAAGCACGCCCCGGTGAATCGTGGCAAGCGGCCGCTGATCGAATC CGCAAAGAATCCCGGCAACCGCCGGCAGCCGGTGCGCCGTCGATTAGGAA GCCGCCCAAGGGCGACGAGCAACCAGATTTTTTCGTTCCGATGCTCTATG ACGTGGGCACCCGCGATAGTCGCAGCATCATGGACGTGGCCGTTTTCCGT CTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTACGAGCTTCC AGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTGT GGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATG AACCGATACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCC ACACGTTGCGGACGTACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGC AGAAAGACGACCTGGTAGAAACCTGCATTCGGTTAAACACCACGCACGTT GCCATGCAGCGTACGAAGAAGGCCAAGAACGGCCGCCTGGTGACGGTATC CGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAAGAGCGAAACCG GGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGCGAG ATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTT TTTGATCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCG CCGCAGGCAAGGCAGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGC AGTGGCAGCGCCGGAGAGTTCAAGAAGTTCTGTTTCACCGTGCGCAAGCT GATCGGGTCAAATGACCTGCCGGAGTACGATTTGAAGGAGGAGGCGGGGC AGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCTGATCGAGGGCGAA GCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATTGCCCT AGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACA TTGGGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAAC CCAAAGCCGTACATTGGGAACCGGTCACACATGTAAGTGACTGATATAAA AGAGAAAAAAGGCGATTTTTCCGCCTAAAACTCTTTAAAACTTATTAAAA CTCTTAAAACCCGCCTGGCCTGTGCATAACTGTCTGGCCAGCGCACAGCC GAAGAGCTGCAAAAAGCGCCTACCCTTCGGTCGCTGCGCTCCCTACGCCC CGCCGCTTCGCGTCGGCCTATCGCGGCCGCTGGCCGCTCAAAAATGGCTG GCCTACGGCCAGGCAATCTACCAGGGCGCGGACAAGCCGCGCCGTCGCCA CTCGACCGCCGGCGCCCACATCAAGGCACCCTGCCTCGCGCGTTTCGGTG ATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCT TGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGC GGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATA GCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGA GAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAA TACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTC GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATAC GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTT CCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTA TCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGC AAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGAT CTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA TTTTGGTCATGCATGATATATCTCCCAATTTGTGTAGGGCTTATTATGCA CGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCTGTGAGCA ATTATGTGCTTAGTGCATCTAATCGCTTGAGTTAACGCCGGCGAAGCGGC GTCGGCTTGAACGAATTTCTAGCTAGACATTATTTGCCGACTACCTTGGT GATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCG AGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGT ATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGC GACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGG ACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAG TTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGG AACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTAT GTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCT GGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTG CAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAA CAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCC GAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAG CCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGC CGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCG AGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTC GGCGATCACCGCTTCCCCCATGATGTTTAACTTTGTTTTAGGGCGACTGC CCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACG GCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACCCCAAA AAAACATGTCATAACAAGAAGCCATGAAAACCGCCACTGCGCCGTTACCA CCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGACGGCAGTTACGC TACTTGCATTACAGCTTACGAACCGAACGAGGCTTATGTCCACTGGGTTC GTGCCCGAATTGATCACAGGCAGCAACGCTCTGTCATCGTTACAATCAAC ATGCTACCCTCCGCGAGATCATCCGTGTTTCAAACCCGGCAGCTTAGTTG CCGTTCTTCCGAATAGCATCGGTAACATGAGCAAAGTCTGCCGCCTTACA ACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAG TGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGTG GCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC ACATTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTAATTCGGGG GATCTATAGGGACTTTAGGTGATCTGGATTTTAGTACTGGATTTTGGTTT TAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACAT ACTAAGGGTTTCTTATATGCTCAACACGTGAGCGAAACCCTATAAGAACC CTAATTCCCTTATCTGGGAACTACTCACACATTATTATGGAGAAACTCGA TGTCGATCGACTCTAGCTAGAACGAATTGTTAGGTGGCGGTACTTGGGTC GATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAG CCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCA CCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAAT GCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCT CACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCA ACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACG GAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGG CTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGAT TTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGA GCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGC TGCTGCGTACCATGGTCGATCGACAGATCTGCGAATCGAGAGAGATAGAT TTGTAGAGAGAGACTGGTGATTTCAGCGTGTCCTCTCCAAATGAAATGAA CTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACG TGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCA TCTTTGGGACCACTGTCGGCAGAGGCATCTTGAACGATAGCCTTTCCTTT ATCGCAATGATGGCATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTT TGATGAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCCGAT ATTACCCTTTGTTGAAAAGTCTCAATAGCCCTTTGGTCTTCTGAGACTGT ATCTTTGATATTCTTGGAGTAGACGAGAGTGTCGTGCTCCACCATGTTAT CACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCC ACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGA GGCATCTTGAACGATAGCCTTTCCTTTATCGCAATGATGGCATTTGTAGG TGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAGTGACAGATAGCTGGG CAATGGAATCCGAGGAGGTTTCCCGATATTACCCTTTGTTGAAAAGTCTC AATAGCCCTTTGGTCTTCTGAGACTGTATCTTTGATATTCTTGGAGTAGA CGAGAGTGTCGTGCTCCACCATGTTGGCAAGCTGCTCTAGCCAATACGCA AACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGAC AGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAG TTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTC GTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGC TATGACCATGATTACGAATTCGGGATATTTCACAAATTGAACATAGACTA CAGAATTTTAGAAAACAAACTTTCTCTCTCTTATCTCACCTTTATCTTTT AGAGAGAAAAAGTTCGATTTCCGGTTGACCGGAATGTATCTTTGTTTTTT TTGTTTTGTAACATATTTCGTTTTCCGATTTAGATCGGATCTCCTTTTCC GTTTTGTCGGACCTTCTTCCGGTTTATCCGGATCTAATAATATCCATCTT AGACTTAGCTAAGTTTGGATCTGTTTTTTGGTTAGCTCTTGTCAATCGCC TCATCATCAGCAAGAAGGTGAAATTTTTGACAAATAAATCTTAGAATCAT GTAGTGTCTTTGGACCTTGGGAATGATAGAAACGATTTGTTATAGCTACT CTATGTATCAGACCCTGACCAAGATCCAACAATCTCATAGGTTTTGTGCA TATGAAACCTTCGACTAACGAGAAGTGGTCTTTTAATGAGAGAGATATCT AAAATGTTATCTTAAAAGCCCACTCAAATCTCAAGGCATAAGGTAGAAAT GCAAATTTGGAAAGTGGGCTGGGCCTTTTGTGGTAAAGGCCTGTAACCTA GCCCAATATTAGCAAAACCCTAGACGCGTACATTGACATATATAAACCCG CCTCCTCCTTGTTTAGGGTTTCTACGTGAGAGAGACGAAACACAAACCAT GGCTTCCTCAGTTCTTTCCTCCGCAGCAGTTGCCACCCGCAGCAATGTTG CTCAAGCTAACATGGTTGCACCTTTCACTGGCCTTAAGTCAGCTGCCTCA TTCCCTGTTTCAAGGAAGCAAAACCTTGACATCACTTCCATTGCCAGCAA CGGCGGAAGAGTGCAATGCATGCAGGTGTGGCCAGCGCGTGACCACATGG TCCTTCATGAGTACGTAAATGCTGCTGGGATTACAATGGCTCCCGATCGG GCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAAGACCCTTCA GCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAAAGCTGGAAC TCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCGGATCAAATC CGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTATGACGAAAG TCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACATTATTGTGA GCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCAAGCCGGGAA TGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCGCTATAACTA TCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTACATGTCGTGG TCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATATCCAGGCGC CATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGCAGAGATTTC ACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAGAAAGGGGAA TAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTTGAGCGGATG CTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCATTAAAACGGC TCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGACCTCGATCAA CGCGCGGTCTATGATTCTAGAAGAGACTCTTATCAAGAATCCCATCTCTT GCTTGCTTTTTTTTGTTGCTTCCCTTTGATAGGGTTTGTTTTTCTTGTTT CAGTGACTTTCTATGTTAAAAGATAATGTCAGTAAAAGGATTTGGTTTTC TATTATTCTGAATCGATTACGGAAGATTCTTGCTTAATTCCAATCTATAC AAGTATCGTGAAATAATGACCGTTTATGTAAGCTTGGCACTGGCCGTCGT TTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGC ACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGCTAGAG CAGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAACTAT CAGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGAAAAGAGCGTT TATTAGAATAACGGATATTTAAAAGGGCGTGAAAAGGTTTATCCGTTCGT CCATTTGTATGTGCATGCCAACCACAGGGTTCCCCTCGGGATCAA

>SEQ ID NO 16 pJF8

AGTACTTTGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACG TTCAGTGCAGCCGTCTTCTGAAAACGACATGTCGCACAAGTCCTAAGTTA CGCGACAGGCTGCCGCCCTGCCCTTTTCCTGGCGTTTTCTTGTCGCGTGT TTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTACGC CATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCA CCGACGACCAGGACTTGACCAACCAACGGGCCGAACTGCACGCGGCCGGC TGCACCAAGCTGTTTTCCGAGAAGATCACCGGCACCAGGCGCGACCGCCC GGAGCTGGCCAGGATGCTTGACCACCTACGCCCTGGCGACGTTGTGACAG TGACCAGGCTAGACCGCCTGGCCCGCAGCACCCGCGACCTACTGGACATT GCCGAGCGCATCCAGGAGGCCGGCGCGGGCCTGCGTAGCCTGGCAGAGCC GTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGTGTTCG CCGGCATTGCCGAGTTCGAGCGTTCCCTAATCATCGACCGCACCCGGAGC GGGCGCGAGGCCGCCAAGGCCCGAGGCGTGAAGTTTGGCCCCCGCCCTAC CCTCACCCCGGCACAGATCGCGCACGCCCGCGAGCTGATCGACCAGGAAG GCCGCACCGTGAAAGAGGCGGCTGCACTGCTTGGCGTGCATCGCTCGACC CTGTACCGCGCACTTGAGCGCAGCGAGGAAGTGACGCCCACCGAGGCCAG GCGGCGCGGTGCCTTCCGTGAGGACGCATTGACCGAGGCCGACGCCCTGG CGGCCGCCGAGAATGAACGCCAAGAGGAACAAGCATGAAACCGCACCAGG ACGGCCAGGACGAACCGTTTTTCATTACCGAAGAGATCGAGGCGGAGATG ATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGTGCG GCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGC CGGCCAGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGA TGTGTATTTGAGTAAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGAT GCGATGAGTAAATAAACAAATACGCAAGGGGAACGCATGAAGGTTATCGC TGTACTTAACCAGAAAGGCGGGTCAGGCAAGACGACCATCGCAACCCATC TAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCTGTTAGTCGATTCC GATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGATCAACC GCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGG CCATCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCG GACTTGGCTGTGTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGT GCAGCCAAGCCCTTACGACATATGGGCCACCGCCGACCTGGTGGAGCTGG TTAAGCAGCGCATTGAGGTCACGGATGGAAGGCTACAAGCGGCCTTTGTC GTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAGGC GCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACGCAGCGCG TGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAA CCCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAA ATCAAAACTCATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCAC AAACACGCTAAGTGCCGGCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAA CGTTGGCCAGCCTGGCAGACACGCCAGCCATGAAGCGGGTCAACTTTCAG TTGCCGGCGGAGGATCACACCAAGCTGAAGATGTACGCGGTACGCCAAGG CAAGACCATTACCGAGCTGCTATCTGAATACATCGCGCAGCTACCAGAGT AAATGAGCAAATGAATAAATGAGTAGATGAATTTTAGCGGCTAAAGGAGG CGGCATGGAAAATCAAGAACAACCAGGCACCGACGCCGTGGAATGCCCCA TGTGTGGAGGAACGGGCGGTTGGCCAGGCGTAAGCGGCTGGGTTGTCTGC CGGCCCTGCAATGGCACTGGAACCCCCAAGCCCGAGGAATCGGCGTGAGC GGTCGCAAACCATCCGGCCCGGTACAAATCGGCGCGGCGCTGGGTGATGA CCTGGTGGAGAAGTTGAAGGCCGCGCAGGCCGCCCAGCGGCAACGCATCG AGGCAGAAGCACGCCCCGGTGAATCGTGGCAAGCGGCCGCTGATCGAATC CGCAAAGAATCCCGGCAACCGCCGGCAGCCGGTGCGCCGTCGATTAGGAA GCCGCCCAAGGGCGACGAGCAACCAGATTTTTTCGTTCCGATGCTCTATG ACGTGGGCACCCGCGATAGTCGCAGCATCATGGACGTGGCCGTTTTCCGT CTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTACGAGCTTCC AGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTGT GGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATG AACCGATACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCC ACACGTTGCGGACGTACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGC AGAAAGACGACCTGGTAGAAACCTGCATTCGGTTAAACACCACGCACGTT GCCATGCAGCGTACGAAGAAGGCCAAGAACGGCCGCCTGGTGACGGTATC CGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAAGAGCGAAACCG GGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGCGAG ATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTT TTTGATCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCG CCGCAGGCAAGGCAGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGC AGTGGCAGCGCCGGAGAGTTCAAGAAGTTCTGTTTCACCGTGCGCAAGCT GATCGGGTCAAATGACCTGCCGGAGTACGATTTGAAGGAGGAGGCGGGGC AGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCTGATCGAGGGCGAA GCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATTGCCCT AGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACA TTGGGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAAC CCAAAGCCGTACATTGGGAACCGGTCACACATGTAAGTGACTGATATAAA AGAGAAAAAAGGCGATTTTTCCGCCTAAAACTCTTTAAAACTTATTAAAA CTCTTAAAACCCGCCTGGCCTGTGCATAACTGTCTGGCCAGCGCACAGCC GAAGAGCTGCAAAAAGCGCCTACCCTTCGGTCGCTGCGCTCCCTACGCCC CGCCGCTTCGCGTCGGCCTATCGCGGCCGCTGGCCGCTCAAAAATGGCTG GCCTACGGCCAGGCAATCTACCAGGGCGCGGACAAGCCGCGCCGTCGCCA CTCGACCGCCGGCGCCCACATCAAGGCACCCTGCCTCGCGCGTTTCGGTG ATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCT TGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGC GGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATA GCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGA GAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAA TACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTC GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATAC GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTT CCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTA TCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGC AAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGAT CTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA TTTTGGTCATGCATGATATATCTCCCAATTTGTGTAGGGCTTATTATGCA CGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCTGTGAGCA ATTATGTGCTTAGTGCATCTAATCGCTTGAGTTAACGCCGGCGAAGCGGC GTCGGCTTGAACGAATTTCTAGCTAGACATTATTTGCCGACTACCTTGGT GATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCG AGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGT ATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGC GACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGG ACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAG TTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGG AACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTAT GTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCT GGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTG CAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAA CAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCC GAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAG CCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGC CGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCG AGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTC GGCGATCACCGCTTCCCCCATGATGTTTAACTTTGTTTTAGGGCGACTGC CCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACG GCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACCCCAAA AAAACATGTCATAACAAGAAGCCATGAAAACCGCCACTGCGCCGTTACCA CCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGACGGCAGTTACGC TACTTGCATTACAGCTTACGAACCGAACGAGGCTTATGTCCACTGGGTTC GTGCCCGAATTGATCACAGGCAGCAACGCTCTGTCATCGTTACAATCAAC ATGCTACCCTCCGCGAGATCATCCGTGTTTCAAACCCGGCAGCTTAGTTG CCGTTCTTCCGAATAGCATCGGTAACATGAGCAAAGTCTGCCGCCTTACA ACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAG TGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGTG GCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC ACATTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTAATTCGGGG GATCTATAGGGACTTTAGGTGATCTGGATTTTAGTACTGGATTTTGGTTT TAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACAT ACTAAGGGTTTCTTATATGCTCAACACGTGAGCGAAACCCTATAAGAACC CTAATTCCCTTATCTGGGAACTACTCACACATTATTATGGAGAAACTCGA TGTCGATCGACTCTAGCTAGAACGAATTGTTAGGTGGCGGTACTTGGGTC GATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAG CCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCA CCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAAT GCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCT CACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCA ACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACG GAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGG CTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGAT TTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGA GCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGC TGCTGCGTACCATGGTCGATCGACAGATCTGCGAATCGAGAGAGATAGAT TTGTAGAGAGAGACTGGTGATTTCAGCGTGTCCTCTCCAAATGAAATGAA CTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACG TGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCA TCTTTGGGACCACTGTCGGCAGAGGCATCTTGAACGATAGCCTTTCCTTT ATCGCAATGATGGCATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTT TGATGAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCCGAT ATTACCCTTTGTTGAAAAGTCTCAATAGCCCTTTGGTCTTCTGAGACTGT ATCTTTGATATTCTTGGAGTAGACGAGAGTGTCGTGCTCCACCATGTTAT CACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCC ACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGA GGCATCTTGAACGATAGCCTTTCCTTTATCGCAATGATGGCATTTGTAGG TGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAGTGACAGATAGCTGGG CAATGGAATCCGAGGAGGTTTCCCGATATTACCCTTTGTTGAAAAGTCTC AATAGCCCTTTGGTCTTCTGAGACTGTATCTTTGATATTCTTGGAGTAGA CGAGAGTGTCGTGCTCCACCATGTTGGCAAGCTGCTCTAGCCAATACGCA AACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGAC AGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAG TTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTC GTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGC TATGACCATGATTACGAATTCGGGATATTTCACAAATTGAACATAGACTA CAGAATTTTAGAAAACAAACTTTCTCTCTCTTATCTCACCTTTATCTTTT AGAGAGAAAAAGTTCGATTTCCGGTTGACCGGAATGTATCTTTGTTTTTT TTGTTTTGTAACATATTTCGTTTTCCGATTTAGATCGGATCTCCTTTTCC GTTTTGTCGGACCTTCTTCCGGTTTATCCGGATCTAATAATATCCATCTT AGACTTAGCTAAGTTTGGATCTGTTTTTTGGTTAGCTCTTGTCAATCGCC TCATCATCAGCAAGAAGGTGAAATTTTTGACAAATAAATCTTAGAATCAT GTAGTGTCTTTGGACCTTGGGAATGATAGAAACGATTTGTTATAGCTACT CTATGTATCAGACCCTGACCAAGATCCAACAATCTCATAGGTTTTGTGCA TATGAAACCTTCGACTAACGAGAAGTGGTCTTTTAATGAGAGAGATATCT AAAATGTTATCTTAAAAGCCCACTCAAATCTCAAGGCATAAGGTAGAAAT GCAAATTTGGAAAGTGGGCTGGGCCTTTTGTGGTAAAGGCCTGTAACCTA GCCCAATATTAGCAAAACCCTAGACGCGTACATTGACATATATAAACCCG CCTCCTCCTTGTTTAGGGTTTCTACGTGAGAGAGACGAAACACAAACCAT GGCTTCCTCAGTTCTTTCCTCCGCAGCAGTTGCCACCCGCAGCAATGTTG CTCAAGCTAACATGGTTGCACCTTTCACTGGCCTTAAGTCAGCTGCCTCA TTCCCTGTTTCAAGGAAGCAAAACCTTGACATCACTTCCATTGCCAGCAA CGGCGGAAGAGTGCAATGCATGCAGGTGTGGCCAGCGCGTGACCACATGG TCCTTCATGAGTACGTAAATGCTGCTGGGATTACAGGTGGAGGGTCTGGT GGCGGATCAATGGCTCCCGATCGGGCCCAAGTAATCATTCGCATTGTGCC AGGAGGTGGAACCAAGACCCTTCAGCAGATAATCAATCAGCTGGAGTACC TGTCCCGAAAGGGAAAGCTGGAACTCCAGCGTTCAGCCCGGCATCTCGAT ATTCCCGTTCCGCCGGATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTAC GGAGGCCGGGATTTATGACGAAAGTCAGTCAGACGATGACAGGCAACAAG ACTTAACAACACACATTATTGTGAGCTTCCCCGCAGGTACCGACCAAACC GCAGCTTATGAAGCAAGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGG ATACGGGGGTGGCCGCTATAACTATCTGACAGCCTACCACGTGGACCGCG ATCATCCACATTTACATGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAG GGGTGGCTGAAAATATCCAGGCGCCATCCCCAGCTGAATTATGACGGCTT ACGGAAAAAGATGGCAGAGATTTCACTTCGTCACGGCATAGTCCTGGATG CGACTTCCCGAGCAGAAAGGGGAATAGCAGAGCGACCAATCACCTATGCT GAATATCGACGTCTTGAGCGGATGCTCGAAGTTATGGCAGAAGTTCGGCC CATAGCCCGATCCATTAAAACGGCTCACGACGATGCGCGAGCGGAATTAA TGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATGATTCTAGAAGAGA CTCTTATCAAGAATCCCATCTCTTGCTTGCTTTTTTTTGTTGCTTCCCTT TGATAGGGTTTGTTTTTCTTGTTTCAGTGACTTTCTATGTTAAAAGATAA TGTCAGTAAAAGGATTTGGTTTTCTATTATTCTGAATCGATTACGGAAGA TTCTTGCTTAATTCCAATCTATACAAGTATCGTGAAATAATGACCGTTTA TGTAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG CAGCCTGAATGGCGAATGCTAGAGCAGCTTGAGCTTGGATCAGATTGTCG TTTCCCGCCTTCAGTTTAAACTATCAGTGTTTGACAGGATATATTGGCGG GTAAACCTAAGAGAAAAGAGCGTTTATTAGAATAACGGATATTTAAAAGG GCGTGAAAAGGTTTATCCGTTCGTCCATTTGTATGTGCATGCCAACCACA GGGTTCCCCTCGGGATCAA

>SEQ ID NO 17 pJF9

AGTACTTTGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACG TTCAGTGCAGCCGTCTTCTGAAAACGACATGTCGCACAAGTCCTAAGTTA CGCGACAGGCTGCCGCCCTGCCCTTTTCCTGGCGTTTTCTTGTCGCGTGT TTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTACGC CATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCA CCGACGACCAGGACTTGACCAACCAACGGGCCGAACTGCACGCGGCCGGC TGCACCAAGCTGTTTTCCGAGAAGATCACCGGCACCAGGCGCGACCGCCC GGAGCTGGCCAGGATGCTTGACCACCTACGCCCTGGCGACGTTGTGACAG TGACCAGGCTAGACCGCCTGGCCCGCAGCACCCGCGACCTACTGGACATT GCCGAGCGCATCCAGGAGGCCGGCGCGGGCCTGCGTAGCCTGGCAGAGCC GTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGTGTTCG CCGGCATTGCCGAGTTCGAGCGTTCCCTAATCATCGACCGCACCCGGAGC GGGCGCGAGGCCGCCAAGGCCCGAGGCGTGAAGTTTGGCCCCCGCCCTAC CCTCACCCCGGCACAGATCGCGCACGCCCGCGAGCTGATCGACCAGGAAG GCCGCACCGTGAAAGAGGCGGCTGCACTGCTTGGCGTGCATCGCTCGACC CTGTACCGCGCACTTGAGCGCAGCGAGGAAGTGACGCCCACCGAGGCCAG GCGGCGCGGTGCCTTCCGTGAGGACGCATTGACCGAGGCCGACGCCCTGG CGGCCGCCGAGAATGAACGCCAAGAGGAACAAGCATGAAACCGCACCAGG ACGGCCAGGACGAACCGTTTTTCATTACCGAAGAGATCGAGGCGGAGATG ATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGTGCG GCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGC CGGCCAGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGA TGTGTATTTGAGTAAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGAT GCGATGAGTAAATAAACAAATACGCAAGGGGAACGCATGAAGGTTATCGC TGTACTTAACCAGAAAGGCGGGTCAGGCAAGACGACCATCGCAACCCATC TAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCTGTTAGTCGATTCC GATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGATCAACC GCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGG CCATCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCG GACTTGGCTGTGTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGT GCAGCCAAGCCCTTACGACATATGGGCCACCGCCGACCTGGTGGAGCTGG TTAAGCAGCGCATTGAGGTCACGGATGGAAGGCTACAAGCGGCCTTTGTC GTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAGGC GCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACGCAGCGCG TGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAA CCCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAA ATCAAAACTCATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCAC AAACACGCTAAGTGCCGGCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAA CGTTGGCCAGCCTGGCAGACACGCCAGCCATGAAGCGGGTCAACTTTCAG TTGCCGGCGGAGGATCACACCAAGCTGAAGATGTACGCGGTACGCCAAGG CAAGACCATTACCGAGCTGCTATCTGAATACATCGCGCAGCTACCAGAGT AAATGAGCAAATGAATAAATGAGTAGATGAATTTTAGCGGCTAAAGGAGG CGGCATGGAAAATCAAGAACAACCAGGCACCGACGCCGTGGAATGCCCCA TGTGTGGAGGAACGGGCGGTTGGCCAGGCGTAAGCGGCTGGGTTGTCTGC CGGCCCTGCAATGGCACTGGAACCCCCAAGCCCGAGGAATCGGCGTGAGC GGTCGCAAACCATCCGGCCCGGTACAAATCGGCGCGGCGCTGGGTGATGA CCTGGTGGAGAAGTTGAAGGCCGCGCAGGCCGCCCAGCGGCAACGCATCG AGGCAGAAGCACGCCCCGGTGAATCGTGGCAAGCGGCCGCTGATCGAATC CGCAAAGAATCCCGGCAACCGCCGGCAGCCGGTGCGCCGTCGATTAGGAA GCCGCCCAAGGGCGACGAGCAACCAGATTTTTTCGTTCCGATGCTCTATG ACGTGGGCACCCGCGATAGTCGCAGCATCATGGACGTGGCCGTTTTCCGT CTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTACGAGCTTCC AGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTGT GGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATG AACCGATACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCC ACACGTTGCGGACGTACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGC AGAAAGACGACCTGGTAGAAACCTGCATTCGGTTAAACACCACGCACGTT GCCATGCAGCGTACGAAGAAGGCCAAGAACGGCCGCCTGGTGACGGTATC CGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAAGAGCGAAACCG GGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGCGAG ATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTT TTTGATCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCG CCGCAGGCAAGGCAGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGC AGTGGCAGCGCCGGAGAGTTCAAGAAGTTCTGTTTCACCGTGCGCAAGCT GATCGGGTCAAATGACCTGCCGGAGTACGATTTGAAGGAGGAGGCGGGGC AGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCTGATCGAGGGCGAA GCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATTGCCCT AGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACA TTGGGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAAC CCAAAGCCGTACATTGGGAACCGGTCACACATGTAAGTGACTGATATAAA AGAGAAAAAAGGCGATTTTTCCGCCTAAAACTCTTTAAAACTTATTAAAA CTCTTAAAACCCGCCTGGCCTGTGCATAACTGTCTGGCCAGCGCACAGCC GAAGAGCTGCAAAAAGCGCCTACCCTTCGGTCGCTGCGCTCCCTACGCCC CGCCGCTTCGCGTCGGCCTATCGCGGCCGCTGGCCGCTCAAAAATGGCTG GCCTACGGCCAGGCAATCTACCAGGGCGCGGACAAGCCGCGCCGTCGCCA CTCGACCGCCGGCGCCCACATCAAGGCACCCTGCCTCGCGCGTTTCGGTG ATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCT TGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGC GGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATA GCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGA GAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAA TACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTC GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATAC GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTT CCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTA TCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGC AAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGAT CTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA TTTTGGTCATGCATGATATATCTCCCAATTTGTGTAGGGCTTATTATGCA CGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCTGTGAGCA ATTATGTGCTTAGTGCATCTAATCGCTTGAGTTAACGCCGGCGAAGCGGC GTCGGCTTGAACGAATTTCTAGCTAGACATTATTTGCCGACTACCTTGGT GATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCG AGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGT ATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGC GACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGG ACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAG TTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGG AACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTAT GTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCT GGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTG CAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAA CAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCC GAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAG CCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGC CGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCG AGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTC GGCGATCACCGCTTCCCCCATGATGTTTAACTTTGTTTTAGGGCGACTGC CCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACG GCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACCCCAAA AAAACATGTCATAACAAGAAGCCATGAAAACCGCCACTGCGCCGTTACCA CCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGACGGCAGTTACGC TACTTGCATTACAGCTTACGAACCGAACGAGGCTTATGTCCACTGGGTTC GTGCCCGAATTGATCACAGGCAGCAACGCTCTGTCATCGTTACAATCAAC ATGCTACCCTCCGCGAGATCATCCGTGTTTCAAACCCGGCAGCTTAGTTG CCGTTCTTCCGAATAGCATCGGTAACATGAGCAAAGTCTGCCGCCTTACA ACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAG TGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGTG GCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC ACATTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTAATTCGGGG GATCTATAGGGACTTTAGGTGATCTGGATTTTAGTACTGGATTTTGGTTT TAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACAT ACTAAGGGTTTCTTATATGCTCAACACGTGAGCGAAACCCTATAAGAACC CTAATTCCCTTATCTGGGAACTACTCACACATTATTATGGAGAAACTCGA TGTCGATCGACTCTAGCTAGAACGAATTGTTAGGTGGCGGTACTTGGGTC GATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAG CCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCA CCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAAT GCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCT CACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCA ACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACG GAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGG CTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGAT TTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGA GCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGC TGCTGCGTACCATGGTCGATCGACAGATCTGCGAATCGAGAGAGATAGAT TTGTAGAGAGAGACTGGTGATTTCAGCGTGTCCTCTCCAAATGAAATGAA CTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACG TGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCA TCTTTGGGACCACTGTCGGCAGAGGCATCTTGAACGATAGCCTTTCCTTT ATCGCAATGATGGCATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTT TGATGAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCCGAT ATTACCCTTTGTTGAAAAGTCTCAATAGCCCTTTGGTCTTCTGAGACTGT ATCTTTGATATTCTTGGAGTAGACGAGAGTGTCGTGCTCCACCATGTTAT CACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCC ACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGA GGCATCTTGAACGATAGCCTTTCCTTTATCGCAATGATGGCATTTGTAGG TGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAGTGACAGATAGCTGGG CAATGGAATCCGAGGAGGTTTCCCGATATTACCCTTTGTTGAAAAGTCTC AATAGCCCTTTGGTCTTCTGAGACTGTATCTTTGATATTCTTGGAGTAGA CGAGAGTGTCGTGCTCCACCATGTTGGCAAGCTGCTCTAGCCAATACGCA AACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGAC AGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAG TTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTC GTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGC TATGACCATGATTACGAATTCGGGATATTTCACAAATTGAACATAGACTA CAGAATTTTAGAAAACAAACTTTCTCTCTCTTATCTCACCTTTATCTTTT AGAGAGAAAAAGTTCGATTTCCGGTTGACCGGAATGTATCTTTGTTTTTT TTGTTTTGTAACATATTTCGTTTTCCGATTTAGATCGGATCTCCTTTTCC GTTTTGTCGGACCTTCTTCCGGTTTATCCGGATCTAATAATATCCATCTT AGACTTAGCTAAGTTTGGATCTGTTTTTTGGTTAGCTCTTGTCAATCGCC TCATCATCAGCAAGAAGGTGAAATTTTTGACAAATAAATCTTAGAATCAT GTAGTGTCTTTGGACCTTGGGAATGATAGAAACGATTTGTTATAGCTACT CTATGTATCAGACCCTGACCAAGATCCAACAATCTCATAGGTTTTGTGCA TATGAAACCTTCGACTAACGAGAAGTGGTCTTTTAATGAGAGAGATATCT AAAATGTTATCTTAAAAGCCCACTCAAATCTCAAGGCATAAGGTAGAAAT GCAAATTTGGAAAGTGGGCTGGGCCTTTTGTGGTAAAGGCCTGTAACCTA GCCCAATATTAGCAAAACCCTAGACGCGTACATTGACATATATAAACCCG CCTCCTCCTTGTTTAGGGTTTCTACGTGAGAGAGACGAAACACAAACCAT GGCTTCCTCAGTTCTTTCCTCCGCAGCAGTTGCCACCCGCAGCAATGTTG CTCAAGCTAACATGGTTGCACCTTTCACTGGCCTTAAGTCAGCTGCCTCA TTCCCTGTTTCAAGGAAGCAAAACCTTGACATCACTTCCATTGCCAGCAA CGGCGGAAGAGTGCAATGCATGCAGGTGTGGCCAGCGATGGCTCCCGATC GGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAAGACCCTT CAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAAAGCTGGA ACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCGGATCAAA TCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTATGACGAA AGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACATTATTGT GAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCAAGCCGGG AATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCGCTATAAC TATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTACATGTCGT GGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATATCCAGGC GCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGCAGAGATT TCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAGAAAGGGG AATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTTGAGCGGA TGCGTGACCACATGGTCCTTCATGAGTACGTAAATGCTGCTGGGATTACA CTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCATTAAAACGGC TCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGACCTCGATCAA CGCGCGGTCTATGATTCTAGAAGAGACTCTTATCAAGAATCCCATCTCTT GCTTGCTTTTTTTTGTTGCTTCCCTTTGATAGGGTTTGTTTTTCTTGTTT CAGTGACTTTCTATGTTAAAAGATAATGTCAGTAAAAGGATTTGGTTTTC TATTATTCTGAATCGATTACGGAAGATTCTTGCTTAATTCCAATCTATAC AAGTATCGTGAAATAATGACCGTTTATGTAAGCTTGGCACTGGCCGTCGT TTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGC ACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGCTAGAG CAGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAACTAT CAGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGAAAAGAGCGTT TATTAGAATAACGGATATTTAAAAGGGCGTGAAAAGGTTTATCCGTTCGT CCATTTGTATGTGCATGCCAACCACAGGGTTCCCCTCGGGATCAA

>SEQ ID NO 18 pJF10

AGTACTTTGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACG TTCAGTGCAGCCGTCTTCTGAAAACGACATGTCGCACAAGTCCTAAGTTA CGCGACAGGCTGCCGCCCTGCCCTTTTCCTGGCGTTTTCTTGTCGCGTGT TTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTACGC CATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCA CCGACGACCAGGACTTGACCAACCAACGGGCCGAACTGCACGCGGCCGGC TGCACCAAGCTGTTTTCCGAGAAGATCACCGGCACCAGGCGCGACCGCCC GGAGCTGGCCAGGATGCTTGACCACCTACGCCCTGGCGACGTTGTGACAG TGACCAGGCTAGACCGCCTGGCCCGCAGCACCCGCGACCTACTGGACATT GCCGAGCGCATCCAGGAGGCCGGCGCGGGCCTGCGTAGCCTGGCAGAGCC GTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGTGTTCG CCGGCATTGCCGAGTTCGAGCGTTCCCTAATCATCGACCGCACCCGGAGC GGGCGCGAGGCCGCCAAGGCCCGAGGCGTGAAGTTTGGCCCCCGCCCTAC CCTCACCCCGGCACAGATCGCGCACGCCCGCGAGCTGATCGACCAGGAAG GCCGCACCGTGAAAGAGGCGGCTGCACTGCTTGGCGTGCATCGCTCGACC CTGTACCGCGCACTTGAGCGCAGCGAGGAAGTGACGCCCACCGAGGCCAG GCGGCGCGGTGCCTTCCGTGAGGACGCATTGACCGAGGCCGACGCCCTGG CGGCCGCCGAGAATGAACGCCAAGAGGAACAAGCATGAAACCGCACCAGG ACGGCCAGGACGAACCGTTTTTCATTACCGAAGAGATCGAGGCGGAGATG ATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGTGCG GCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGC CGGCCAGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGA TGTGTATTTGAGTAAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGAT GCGATGAGTAAATAAACAAATACGCAAGGGGAACGCATGAAGGTTATCGC TGTACTTAACCAGAAAGGCGGGTCAGGCAAGACGACCATCGCAACCCATC TAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCTGTTAGTCGATTCC GATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGATCAACC GCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGG CCATCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCG GACTTGGCTGTGTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGT GCAGCCAAGCCCTTACGACATATGGGCCACCGCCGACCTGGTGGAGCTGG TTAAGCAGCGCATTGAGGTCACGGATGGAAGGCTACAAGCGGCCTTTGTC GTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAGGC GCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACGCAGCGCG TGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAA CCCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAA ATCAAAACTCATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCAC AAACACGCTAAGTGCCGGCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAA CGTTGGCCAGCCTGGCAGACACGCCAGCCATGAAGCGGGTCAACTTTCAG TTGCCGGCGGAGGATCACACCAAGCTGAAGATGTACGCGGTACGCCAAGG CAAGACCATTACCGAGCTGCTATCTGAATACATCGCGCAGCTACCAGAGT AAATGAGCAAATGAATAAATGAGTAGATGAATTTTAGCGGCTAAAGGAGG CGGCATGGAAAATCAAGAACAACCAGGCACCGACGCCGTGGAATGCCCCA TGTGTGGAGGAACGGGCGGTTGGCCAGGCGTAAGCGGCTGGGTTGTCTGC CGGCCCTGCAATGGCACTGGAACCCCCAAGCCCGAGGAATCGGCGTGAGC GGTCGCAAACCATCCGGCCCGGTACAAATCGGCGCGGCGCTGGGTGATGA CCTGGTGGAGAAGTTGAAGGCCGCGCAGGCCGCCCAGCGGCAACGCATCG AGGCAGAAGCACGCCCCGGTGAATCGTGGCAAGCGGCCGCTGATCGAATC CGCAAAGAATCCCGGCAACCGCCGGCAGCCGGTGCGCCGTCGATTAGGAA GCCGCCCAAGGGCGACGAGCAACCAGATTTTTTCGTTCCGATGCTCTATG ACGTGGGCACCCGCGATAGTCGCAGCATCATGGACGTGGCCGTTTTCCGT CTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTACGAGCTTCC AGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTGT GGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATG AACCGATACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCC ACACGTTGCGGACGTACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGC AGAAAGACGACCTGGTAGAAACCTGCATTCGGTTAAACACCACGCACGTT GCCATGCAGCGTACGAAGAAGGCCAAGAACGGCCGCCTGGTGACGGTATC CGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAAGAGCGAAACCG GGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGCGAG ATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTT TTTGATCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCG CCGCAGGCAAGGCAGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGC AGTGGCAGCGCCGGAGAGTTCAAGAAGTTCTGTTTCACCGTGCGCAAGCT GATCGGGTCAAATGACCTGCCGGAGTACGATTTGAAGGAGGAGGCGGGGC AGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCTGATCGAGGGCGAA GCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATTGCCCT AGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACA TTGGGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAAC CCAAAGCCGTACATTGGGAACCGGTCACACATGTAAGTGACTGATATAAA AGAGAAAAAAGGCGATTTTTCCGCCTAAAACTCTTTAAAACTTATTAAAA CTCTTAAAACCCGCCTGGCCTGTGCATAACTGTCTGGCCAGCGCACAGCC GAAGAGCTGCAAAAAGCGCCTACCCTTCGGTCGCTGCGCTCCCTACGCCC CGCCGCTTCGCGTCGGCCTATCGCGGCCGCTGGCCGCTCAAAAATGGCTG GCCTACGGCCAGGCAATCTACCAGGGCGCGGACAAGCCGCGCCGTCGCCA CTCGACCGCCGGCGCCCACATCAAGGCACCCTGCCTCGCGCGTTTCGGTG ATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCT TGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGC GGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATA GCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGA GAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAA TACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTC GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATAC GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTT CCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTA TCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGC AAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGAT CTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA TTTTGGTCATGCATGATATATCTCCCAATTTGTGTAGGGCTTATTATGCA CGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCTGTGAGCA ATTATGTGCTTAGTGCATCTAATCGCTTGAGTTAACGCCGGCGAAGCGGC GTCGGCTTGAACGAATTTCTAGCTAGACATTATTTGCCGACTACCTTGGT GATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCG AGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGT ATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGC GACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGG ACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAG TTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGG AACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTAT GTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCT GGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTG CAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAA CAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCC GAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAG CCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGC CGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCG AGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTC GGCGATCACCGCTTCCCCCATGATGTTTAACTTTGTTTTAGGGCGACTGC CCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACG GCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACCCCAAA AAAACATGTCATAACAAGAAGCCATGAAAACCGCCACTGCGCCGTTACCA CCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGACGGCAGTTACGC TACTTGCATTACAGCTTACGAACCGAACGAGGCTTATGTCCACTGGGTTC GTGCCCGAATTGATCACAGGCAGCAACGCTCTGTCATCGTTACAATCAAC ATGCTACCCTCCGCGAGATCATCCGTGTTTCAAACCCGGCAGCTTAGTTG CCGTTCTTCCGAATAGCATCGGTAACATGAGCAAAGTCTGCCGCCTTACA ACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAG TGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGTG GCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC ACATTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTAATTCGGGG GATCTATAGGGACTTTAGGTGATCTGGATTTTAGTACTGGATTTTGGTTT TAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACAT ACTAAGGGTTTCTTATATGCTCAACACGTGAGCGAAACCCTATAAGAACC CTAATTCCCTTATCTGGGAACTACTCACACATTATTATGGAGAAACTCGA TGTCGATCGACTCTAGCTAGAACGAATTGTTAGGTGGCGGTACTTGGGTC GATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAG CCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCA CCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAAT GCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCT CACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCA ACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACG GAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGG CTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGAT TTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGA GCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGC TGCTGCGTACCATGGTCGATCGACAGATCTGCGAATCGAGAGAGATAGAT TTGTAGAGAGAGACTGGTGATTTCAGCGTGTCCTCTCCAAATGAAATGAA CTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACG TGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCA TCTTTGGGACCACTGTCGGCAGAGGCATCTTGAACGATAGCCTTTCCTTT ATCGCAATGATGGCATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTT TGATGAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCCGAT ATTACCCTTTGTTGAAAAGTCTCAATAGCCCTTTGGTCTTCTGAGACTGT ATCTTTGATATTCTTGGAGTAGACGAGAGTGTCGTGCTCCACCATGTTAT CACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCC ACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGA GGCATCTTGAACGATAGCCTTTCCTTTATCGCAATGATGGCATTTGTAGG TGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAGTGACAGATAGCTGGG CAATGGAATCCGAGGAGGTTTCCCGATATTACCCTTTGTTGAAAAGTCTC AATAGCCCTTTGGTCTTCTGAGACTGTATCTTTGATATTCTTGGAGTAGA CGAGAGTGTCGTGCTCCACCATGTTGGCAAGCTGCTCTAGCCAATACGCA AACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGAC AGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAG TTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTC GTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGC TATGACCATGATTACGAATTCGGGATATTTCACAAATTGAACATAGACTA CAGAATTTTAGAAAACAAACTTTCTCTCTCTTATCTCACCTTTATCTTTT AGAGAGAAAAAGTTCGATTTCCGGTTGACCGGAATGTATCTTTGTTTTTT TTGTTTTGTAACATATTTCGTTTTCCGATTTAGATCGGATCTCCTTTTCC GTTTTGTCGGACCTTCTTCCGGTTTATCCGGATCTAATAATATCCATCTT AGACTTAGCTAAGTTTGGATCTGTTTTTTGGTTAGCTCTTGTCAATCGCC TCATCATCAGCAAGAAGGTGAAATTTTTGACAAATAAATCTTAGAATCAT GTAGTGTCTTTGGACCTTGGGAATGATAGAAACGATTTGTTATAGCTACT CTATGTATCAGACCCTGACCAAGATCCAACAATCTCATAGGTTTTGTGCA TATGAAACCTTCGACTAACGAGAAGTGGTCTTTTAATGAGAGAGATATCT AAAATGTTATCTTAAAAGCCCACTCAAATCTCAAGGCATAAGGTAGAAAT GCAAATTTGGAAAGTGGGCTGGGCCTTTTGTGGTAAAGGCCTGTAACCTA GCCCAATATTAGCAAAACCCTAGACGCGTACATTGACATATATAAACCCG CCTCCTCCTTGTTTAGGGTTTCTACGTGAGAGAGACGAAACACAAACCAT GGCTTCCTCAGTTCTTTCCTCCGCAGCAGTTGCCACCCGCAGCAATGTTG CTCAAGCTAACATGGTTGCACCTTTCACTGGCCTTAAGTCAGCTGCCTCA TTCCCTGTTTCAAGGAAGCAAAACCTTGACATCACTTCCATTGCCAGCAA CGGCGGAAGAGTGCAATGCATGCAGGTGTGGCCAGCGATGGCTCCCGATC GGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAAGACCCTT CAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAAAGCTGGA ACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCGGATCAAA TCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTATGACGAA AGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACATTATTGT GAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCAAGCCGGG AATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCGCTATAAC TATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTACATGTCGT GGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATATCCAGGC GCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGCAGAGATT TCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAGAAAGGGG AATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTTGAGCGGA TGGGTGGAGGGTCTGGTGGCGGATCACGTGACCACATGGTCCTTCATGAG TACGTAAATGCTGCTGGGATTACACTCGAAGTTATGGCAGAAGTTCGGCC CATAGCCCGATCCATTAAAACGGCTCACGACGATGCGCGAGCGGAATTAA TGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATGATTCTAGAAGAGA CTCTTATCAAGAATCCCATCTCTTGCTTGCTTTTTTTTGTTGCTTCCCTT TGATAGGGTTTGTTTTTCTTGTTTCAGTGACTTTCTATGTTAAAAGATAA TGTCAGTAAAAGGATTTGGTTTTCTATTATTCTGAATCGATTACGGAAGA TTCTTGCTTAATTCCAATCTATACAAGTATCGTGAAATAATGACCGTTTA TGTAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG CAGCCTGAATGGCGAATGCTAGAGCAGCTTGAGCTTGGATCAGATTGTCG TTTCCCGCCTTCAGTTTAAACTATCAGTGTTTGACAGGATATATTGGCGG GTAAACCTAAGAGAAAAGAGCGTTTATTAGAATAACGGATATTTAAAAGG GCGTGAAAAGGTTTATCCGTTCGTCCATTTGTATGTGCATGCCAACCACA GGGTTCCCCTCGGGATCAA

>SEQ ID NO 19 pJF1 1

AGTACTTTGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACG TTCAGTGCAGCCGTCTTCTGAAAACGACATGTCGCACAAGTCCTAAGTTA CGCGACAGGCTGCCGCCCTGCCCTTTTCCTGGCGTTTTCTTGTCGCGTGT TTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTACGC CATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCA CCGACGACCAGGACTTGACCAACCAACGGGCCGAACTGCACGCGGCCGGC TGCACCAAGCTGTTTTCCGAGAAGATCACCGGCACCAGGCGCGACCGCCC GGAGCTGGCCAGGATGCTTGACCACCTACGCCCTGGCGACGTTGTGACAG TGACCAGGCTAGACCGCCTGGCCCGCAGCACCCGCGACCTACTGGACATT GCCGAGCGCATCCAGGAGGCCGGCGCGGGCCTGCGTAGCCTGGCAGAGCC GTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGTGTTCG CCGGCATTGCCGAGTTCGAGCGTTCCCTAATCATCGACCGCACCCGGAGC GGGCGCGAGGCCGCCAAGGCCCGAGGCGTGAAGTTTGGCCCCCGCCCTAC CCTCACCCCGGCACAGATCGCGCACGCCCGCGAGCTGATCGACCAGGAAG GCCGCACCGTGAAAGAGGCGGCTGCACTGCTTGGCGTGCATCGCTCGACC CTGTACCGCGCACTTGAGCGCAGCGAGGAAGTGACGCCCACCGAGGCCAG GCGGCGCGGTGCCTTCCGTGAGGACGCATTGACCGAGGCCGACGCCCTGG CGGCCGCCGAGAATGAACGCCAAGAGGAACAAGCATGAAACCGCACCAGG ACGGCCAGGACGAACCGTTTTTCATTACCGAAGAGATCGAGGCGGAGATG ATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGTGCG GCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGC CGGCCAGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGA TGTGTATTTGAGTAAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGAT GCGATGAGTAAATAAACAAATACGCAAGGGGAACGCATGAAGGTTATCGC TGTACTTAACCAGAAAGGCGGGTCAGGCAAGACGACCATCGCAACCCATC TAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCTGTTAGTCGATTCC GATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGATCAACC GCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGG CCATCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCG GACTTGGCTGTGTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGT GCAGCCAAGCCCTTACGACATATGGGCCACCGCCGACCTGGTGGAGCTGG TTAAGCAGCGCATTGAGGTCACGGATGGAAGGCTACAAGCGGCCTTTGTC GTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAGGC GCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACGCAGCGCG TGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAA CCCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAA ATCAAAACTCATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCAC AAACACGCTAAGTGCCGGCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAA CGTTGGCCAGCCTGGCAGACACGCCAGCCATGAAGCGGGTCAACTTTCAG TTGCCGGCGGAGGATCACACCAAGCTGAAGATGTACGCGGTACGCCAAGG CAAGACCATTACCGAGCTGCTATCTGAATACATCGCGCAGCTACCAGAGT AAATGAGCAAATGAATAAATGAGTAGATGAATTTTAGCGGCTAAAGGAGG CGGCATGGAAAATCAAGAACAACCAGGCACCGACGCCGTGGAATGCCCCA TGTGTGGAGGAACGGGCGGTTGGCCAGGCGTAAGCGGCTGGGTTGTCTGC CGGCCCTGCAATGGCACTGGAACCCCCAAGCCCGAGGAATCGGCGTGAGC GGTCGCAAACCATCCGGCCCGGTACAAATCGGCGCGGCGCTGGGTGATGA CCTGGTGGAGAAGTTGAAGGCCGCGCAGGCCGCCCAGCGGCAACGCATCG AGGCAGAAGCACGCCCCGGTGAATCGTGGCAAGCGGCCGCTGATCGAATC CGCAAAGAATCCCGGCAACCGCCGGCAGCCGGTGCGCCGTCGATTAGGAA GCCGCCCAAGGGCGACGAGCAACCAGATTTTTTCGTTCCGATGCTCTATG ACGTGGGCACCCGCGATAGTCGCAGCATCATGGACGTGGCCGTTTTCCGT CTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTACGAGCTTCC AGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTGT GGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATG AACCGATACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCC ACACGTTGCGGACGTACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGC AGAAAGACGACCTGGTAGAAACCTGCATTCGGTTAAACACCACGCACGTT GCCATGCAGCGTACGAAGAAGGCCAAGAACGGCCGCCTGGTGACGGTATC CGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAAGAGCGAAACCG GGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGCGAG ATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTT TTTGATCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCG CCGCAGGCAAGGCAGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGC AGTGGCAGCGCCGGAGAGTTCAAGAAGTTCTGTTTCACCGTGCGCAAGCT GATCGGGTCAAATGACCTGCCGGAGTACGATTTGAAGGAGGAGGCGGGGC AGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCTGATCGAGGGCGAA GCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATTGCCCT AGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACA TTGGGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAAC CCAAAGCCGTACATTGGGAACCGGTCACACATGTAAGTGACTGATATAAA AGAGAAAAAAGGCGATTTTTCCGCCTAAAACTCTTTAAAACTTATTAAAA CTCTTAAAACCCGCCTGGCCTGTGCATAACTGTCTGGCCAGCGCACAGCC GAAGAGCTGCAAAAAGCGCCTACCCTTCGGTCGCTGCGCTCCCTACGCCC CGCCGCTTCGCGTCGGCCTATCGCGGCCGCTGGCCGCTCAAAAATGGCTG GCCTACGGCCAGGCAATCTACCAGGGCGCGGACAAGCCGCGCCGTCGCCA CTCGACCGCCGGCGCCCACATCAAGGCACCCTGCCTCGCGCGTTTCGGTG ATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCT TGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGC GGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATA GCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGA GAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAA TACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTC GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATAC GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTT CCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTA TCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGC AAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGAT CTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA TTTTGGTCATGCATGATATATCTCCCAATTTGTGTAGGGCTTATTATGCA CGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCTGTGAGCA ATTATGTGCTTAGTGCATCTAATCGCTTGAGTTAACGCCGGCGAAGCGGC GTCGGCTTGAACGAATTTCTAGCTAGACATTATTTGCCGACTACCTTGGT GATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCG AGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGT ATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGC GACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGG ACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAG TTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGG AACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTAT GTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCT GGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTG CAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAA CAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCC GAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAG CCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGC CGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCG AGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTC GGCGATCACCGCTTCCCCCATGATGTTTAACTTTGTTTTAGGGCGACTGC CCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACG GCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACCCCAAA AAAACATGTCATAACAAGAAGCCATGAAAACCGCCACTGCGCCGTTACCA CCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGACGGCAGTTACGC TACTTGCATTACAGCTTACGAACCGAACGAGGCTTATGTCCACTGGGTTC GTGCCCGAATTGATCACAGGCAGCAACGCTCTGTCATCGTTACAATCAAC ATGCTACCCTCCGCGAGATCATCCGTGTTTCAAACCCGGCAGCTTAGTTG CCGTTCTTCCGAATAGCATCGGTAACATGAGCAAAGTCTGCCGCCTTACA ACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAG TGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGTG GCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC ACATTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTAATTCGGGG GATCTATAGGGACTTTAGGTGATCTGGATTTTAGTACTGGATTTTGGTTT TAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACAT ACTAAGGGTTTCTTATATGCTCAACACGTGAGCGAAACCCTATAAGAACC CTAATTCCCTTATCTGGGAACTACTCACACATTATTATGGAGAAACTCGA TGTCGATCGACTCTAGCTAGAACGAATTGTTAGGTGGCGGTACTTGGGTC GATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAG CCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCA CCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAAT GCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCT CACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCA ACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACG GAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGG CTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGAT TTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGA GCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGC TGCTGCGTACCATGGTCGATCGACAGATCTGCGAATCGAGAGAGATAGAT TTGTAGAGAGAGACTGGTGATTTCAGCGTGTCCTCTCCAAATGAAATGAA CTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACG TGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCA TCTTTGGGACCACTGTCGGCAGAGGCATCTTGAACGATAGCCTTTCCTTT ATCGCAATGATGGCATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTT TGATGAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCCGAT ATTACCCTTTGTTGAAAAGTCTCAATAGCCCTTTGGTCTTCTGAGACTGT ATCTTTGATATTCTTGGAGTAGACGAGAGTGTCGTGCTCCACCATGTTAT CACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCC ACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGA GGCATCTTGAACGATAGCCTTTCCTTTATCGCAATGATGGCATTTGTAGG TGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAGTGACAGATAGCTGGG CAATGGAATCCGAGGAGGTTTCCCGATATTACCCTTTGTTGAAAAGTCTC AATAGCCCTTTGGTCTTCTGAGACTGTATCTTTGATATTCTTGGAGTAGA CGAGAGTGTCGTGCTCCACCATGTTGGCAAGCTGCTCTAGCCAATACGCA AACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGAC AGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAG TTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTC GTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGC TATGACCATGATTACGAATTCGGGATATTTCACAAATTGAACATAGACTA CAGAATTTTAGAAAACAAACTTTCTCTCTCTTATCTCACCTTTATCTTTT AGAGAGAAAAAGTTCGATTTCCGGTTGACCGGAATGTATCTTTGTTTTTT TTGTTTTGTAACATATTTCGTTTTCCGATTTAGATCGGATCTCCTTTTCC GTTTTGTCGGACCTTCTTCCGGTTTATCCGGATCTAATAATATCCATCTT AGACTTAGCTAAGTTTGGATCTGTTTTTTGGTTAGCTCTTGTCAATCGCC TCATCATCAGCAAGAAGGTGAAATTTTTGACAAATAAATCTTAGAATCAT GTAGTGTCTTTGGACCTTGGGAATGATAGAAACGATTTGTTATAGCTACT CTATGTATCAGACCCTGACCAAGATCCAACAATCTCATAGGTTTTGTGCA TATGAAACCTTCGACTAACGAGAAGTGGTCTTTTAATGAGAGAGATATCT AAAATGTTATCTTAAAAGCCCACTCAAATCTCAAGGCATAAGGTAGAAAT GCAAATTTGGAAAGTGGGCTGGGCCTTTTGTGGTAAAGGCCTGTAACCTA GCCCAATATTAGCAAAACCCTAGACGCGTACATTGACATATATAAACCCG CCTCCTCCTTGTTTAGGGTTTCTACGTGAGAGAGACGAAACACAAACCAT GGCTTCCTCAGTTCTTTCCTCCGCAGCAGTTGCCACCCGCAGCAATGTTG CTCAAGCTAACATGGTTGCACCTTTCACTGGCCTTAAGTCAGCTGCCTCA TTCCCTGTTTCAAGGAAGCAAAACCTTGACATCACTTCCATTGCCAGCAA CGGCGGAAGAGTGCAATGCATGCAGGTGTGGCCAGCGATGGCTCCCGATC GGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAAGACCCTT CAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAAAGCTGGA ACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCGGATCAAA TCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTATGACGAA AGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACATTATTGT GAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCAAGCCGGG AATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCGCTATAAC TATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTACATGTCGT GGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATATCCAGGC GCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGCAGAGATT TCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAGAAAGGGG AATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTTGAGCGGA TGCGTGACCACATGGTCCTTCATGAGTACGTAAATGCTGCTGGGATTACA GGTGGAGGGTCTGGTGGCGGATCACTCGAAGTTATGGCAGAAGTTCGGCC CATAGCCCGATCCATTAAAACGGCTCACGACGATGCGCGAGCGGAATTAA TGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATGATTCTAGAAGAGA CTCTTATCAAGAATCCCATCTCTTGCTTGCTTTTTTTTGTTGCTTCCCTT TGATAGGGTTTGTTTTTCTTGTTTCAGTGACTTTCTATGTTAAAAGATAA TGTCAGTAAAAGGATTTGGTTTTCTATTATTCTGAATCGATTACGGAAGA TTCTTGCTTAATTCCAATCTATACAAGTATCGTGAAATAATGACCGTTTA TGTAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG CAGCCTGAATGGCGAATGCTAGAGCAGCTTGAGCTTGGATCAGATTGTCG TTTCCCGCCTTCAGTTTAAACTATCAGTGTTTGACAGGATATATTGGCGG GTAAACCTAAGAGAAAAGAGCGTTTATTAGAATAACGGATATTTAAAAGG GCGTGAAAAGGTTTATCCGTTCGTCCATTTGTATGTGCATGCCAACCACA GGGTTCCCCTCGGGATCAA

> SEQ ID NO 20 pJF12

AGTACTTTGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACG TTCAGTGCAGCCGTCTTCTGAAAACGACATGTCGCACAAGTCCTAAGTTA CGCGACAGGCTGCCGCCCTGCCCTTTTCCTGGCGTTTTCTTGTCGCGTGT TTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTACGC CATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCA CCGACGACCAGGACTTGACCAACCAACGGGCCGAACTGCACGCGGCCGGC TGCACCAAGCTGTTTTCCGAGAAGATCACCGGCACCAGGCGCGACCGCCC GGAGCTGGCCAGGATGCTTGACCACCTACGCCCTGGCGACGTTGTGACAG TGACCAGGCTAGACCGCCTGGCCCGCAGCACCCGCGACCTACTGGACATT GCCGAGCGCATCCAGGAGGCCGGCGCGGGCCTGCGTAGCCTGGCAGAGCC GTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGTGTTCG CCGGCATTGCCGAGTTCGAGCGTTCCCTAATCATCGACCGCACCCGGAGC GGGCGCGAGGCCGCCAAGGCCCGAGGCGTGAAGTTTGGCCCCCGCCCTAC CCTCACCCCGGCACAGATCGCGCACGCCCGCGAGCTGATCGACCAGGAAG GCCGCACCGTGAAAGAGGCGGCTGCACTGCTTGGCGTGCATCGCTCGACC CTGTACCGCGCACTTGAGCGCAGCGAGGAAGTGACGCCCACCGAGGCCAG GCGGCGCGGTGCCTTCCGTGAGGACGCATTGACCGAGGCCGACGCCCTGG CGGCCGCCGAGAATGAACGCCAAGAGGAACAAGCATGAAACCGCACCAGG ACGGCCAGGACGAACCGTTTTTCATTACCGAAGAGATCGAGGCGGAGATG ATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGTGCG GCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGC CGGCCAGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGA TGTGTATTTGAGTAAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGAT GCGATGAGTAAATAAACAAATACGCAAGGGGAACGCATGAAGGTTATCGC TGTACTTAACCAGAAAGGCGGGTCAGGCAAGACGACCATCGCAACCCATC TAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCTGTTAGTCGATTCC GATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGATCAACC GCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGG CCATCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCG GACTTGGCTGTGTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGT GCAGCCAAGCCCTTACGACATATGGGCCACCGCCGACCTGGTGGAGCTGG TTAAGCAGCGCATTGAGGTCACGGATGGAAGGCTACAAGCGGCCTTTGTC GTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAGGC GCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACGCAGCGCG TGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAA CCCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAA ATCAAAACTCATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCAC AAACACGCTAAGTGCCGGCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAA CGTTGGCCAGCCTGGCAGACACGCCAGCCATGAAGCGGGTCAACTTTCAG TTGCCGGCGGAGGATCACACCAAGCTGAAGATGTACGCGGTACGCCAAGG CAAGACCATTACCGAGCTGCTATCTGAATACATCGCGCAGCTACCAGAGT AAATGAGCAAATGAATAAATGAGTAGATGAATTTTAGCGGCTAAAGGAGG CGGCATGGAAAATCAAGAACAACCAGGCACCGACGCCGTGGAATGCCCCA TGTGTGGAGGAACGGGCGGTTGGCCAGGCGTAAGCGGCTGGGTTGTCTGC CGGCCCTGCAATGGCACTGGAACCCCCAAGCCCGAGGAATCGGCGTGAGC GGTCGCAAACCATCCGGCCCGGTACAAATCGGCGCGGCGCTGGGTGATGA CCTGGTGGAGAAGTTGAAGGCCGCGCAGGCCGCCCAGCGGCAACGCATCG AGGCAGAAGCACGCCCCGGTGAATCGTGGCAAGCGGCCGCTGATCGAATC CGCAAAGAATCCCGGCAACCGCCGGCAGCCGGTGCGCCGTCGATTAGGAA GCCGCCCAAGGGCGACGAGCAACCAGATTTTTTCGTTCCGATGCTCTATG ACGTGGGCACCCGCGATAGTCGCAGCATCATGGACGTGGCCGTTTTCCGT CTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTACGAGCTTCC AGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTGT GGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATG AACCGATACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCC ACACGTTGCGGACGTACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGC AGAAAGACGACCTGGTAGAAACCTGCATTCGGTTAAACACCACGCACGTT GCCATGCAGCGTACGAAGAAGGCCAAGAACGGCCGCCTGGTGACGGTATC CGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAAGAGCGAAACCG GGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGCGAG ATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTT TTTGATCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCG CCGCAGGCAAGGCAGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGC AGTGGCAGCGCCGGAGAGTTCAAGAAGTTCTGTTTCACCGTGCGCAAGCT GATCGGGTCAAATGACCTGCCGGAGTACGATTTGAAGGAGGAGGCGGGGC AGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCTGATCGAGGGCGAA GCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATTGCCCT AGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACA TTGGGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAAC CCAAAGCCGTACATTGGGAACCGGTCACACATGTAAGTGACTGATATAAA AGAGAAAAAAGGCGATTTTTCCGCCTAAAACTCTTTAAAACTTATTAAAA CTCTTAAAACCCGCCTGGCCTGTGCATAACTGTCTGGCCAGCGCACAGCC GAAGAGCTGCAAAAAGCGCCTACCCTTCGGTCGCTGCGCTCCCTACGCCC CGCCGCTTCGCGTCGGCCTATCGCGGCCGCTGGCCGCTCAAAAATGGCTG GCCTACGGCCAGGCAATCTACCAGGGCGCGGACAAGCCGCGCCGTCGCCA CTCGACCGCCGGCGCCCACATCAAGGCACCCTGCCTCGCGCGTTTCGGTG ATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCT TGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGC GGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATA GCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGA GAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAA TACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTC GGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATAC GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAA GGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTT CCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCT GGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGT GTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTA TCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAG TTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGG TATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGC AAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGAT CTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA TTTTGGTCATGCATGATATATCTCCCAATTTGTGTAGGGCTTATTATGCA CGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCTGTGAGCA ATTATGTGCTTAGTGCATCTAATCGCTTGAGTTAACGCCGGCGAAGCGGC GTCGGCTTGAACGAATTTCTAGCTAGACATTATTTGCCGACTACCTTGGT GATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCG AGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGT ATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGC GACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGG ACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAG TTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGG AACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTAT GTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCT GGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTG CAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAA CAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCC GAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAG CCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGC CGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCG AGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTC GGCGATCACCGCTTCCCCCATGATGTTTAACTTTGTTTTAGGGCGACTGC CCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACG GCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACCCCAAA AAAACATGTCATAACAAGAAGCCATGAAAACCGCCACTGCGCCGTTACCA CCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGACGGCAGTTACGC TACTTGCATTACAGCTTACGAACCGAACGAGGCTTATGTCCACTGGGTTC GTGCCCGAATTGATCACAGGCAGCAACGCTCTGTCATCGTTACAATCAAC ATGCTACCCTCCGCGAGATCATCCGTGTTTCAAACCCGGCAGCTTAGTTG CCGTTCTTCCGAATAGCATCGGTAACATGAGCAAAGTCTGCCGCCTTACA ACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAG TGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGTG GCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAAC ACATTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTAATTCGGGG GATCTATAGGGACTTTAGGTGATCTGGATTTTAGTACTGGATTTTGGTTT TAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACAT ACTAAGGGTTTCTTATATGCTCAACACGTGAGCGAAACCCTATAAGAACC CTAATTCCCTTATCTGGGAACTACTCACACATTATTATGGAGAAACTCGA TGTCGATCGACTCTAGCTAGAACGAATTGTTAGGTGGCGGTACTTGGGTC GATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAG CCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCA CCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAAT GCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCT CACTACGCGGCTGCTCAAACCTGGGCAGAACGTAAGCCGCGAGAGCGCCA ACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACG GAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGG CTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGAT TTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGA GCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGC TGCTGCGTACCATGGTCGATCGACAGATCTGCGAATCGAGAGAGATAGAT TTGTAGAGAGAGACTGGTGATTTCAGCGTGTCCTCTCCAAATGAAATGAA CTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACG TGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCA TCTTTGGGACCACTGTCGGCAGAGGCATCTTGAACGATAGCCTTTCCTTT ATCGCAATGATGGCATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTT TGATGAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCCGAT ATTACCCTTTGTTGAAAAGTCTCAATAGCCCTTTGGTCTTCTGAGACTGT ATCTTTGATATTCTTGGAGTAGACGAGAGTGTCGTGCTCCACCATGTTAT CACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCC ACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGA GGCATCTTGAACGATAGCCTTTCCTTTATCGCAATGATGGCATTTGTAGG TGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAGTGACAGATAGCTGGG CAATGGAATCCGAGGAGGTTTCCCGATATTACCCTTTGTTGAAAAGTCTC AATAGCCCTTTGGTCTTCTGAGACTGTATCTTTGATATTCTTGGAGTAGA CGAGAGTGTCGTGCTCCACCATGTTGGCAAGCTGCTCTAGCCAATACGCA AACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGAC AGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAG TTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTC GTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGC TATGACCATGATTACGAATTCGGGATATTTCACAAATTGAACATAGACTA CAGAATTTTAGAAAACAAACTTTCTCTCTCTTATCTCACCTTTATCTTTT AGAGAGAAAAAGTTCGATTTCCGGTTGACCGGAATGTATCTTTGTTTTTT TTGTTTTGTAACATATTTCGTTTTCCGATTTAGATCGGATCTCCTTTTCC GTTTTGTCGGACCTTCTTCCGGTTTATCCGGATCTAATAATATCCATCTT AGACTTAGCTAAGTTTGGATCTGTTTTTTGGTTAGCTCTTGTCAATCGCC TCATCATCAGCAAGAAGGTGAAATTTTTGACAAATAAATCTTAGAATCAT GTAGTGTCTTTGGACCTTGGGAATGATAGAAACGATTTGTTATAGCTACT CTATGTATCAGACCCTGACCAAGATCCAACAATCTCATAGGTTTTGTGCA TATGAAACCTTCGACTAACGAGAAGTGGTCTTTTAATGAGAGAGATATCT AAAATGTTATCTTAAAAGCCCACTCAAATCTCAAGGCATAAGGTAGAAAT GCAAATTTGGAAAGTGGGCTGGGCCTTTTGTGGTAAAGGCCTGTAACCTA GCCCAATATTAGCAAAACCCTAGACGCGTACATTGACATATATAAACCCG CCTCCTCCTTGTTTAGGGTTTCTACGTGAGAGAGACGAAACACAAACCAT GGCTTCCTCAGTTCTTTCCTCCGCAGCAGTTGCCACCCGCAGCAATGTTG CTCAAGCTAACATGGTTGCACCTTTCACTGGCCTTAAGTCAGCTGCCTCA TTCCCTGTTTCAAGGAAGCAAAACCTTGACATCACTTCCATTGCCAGCAA CGGCGGAAGAGTGCAATGCATGCAGGTGTGGCCAGCGATGGCTCCCGATC GGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAAGACCCTT CAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAAAGCTGGA ACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCGGATCAAA TCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTATGACGAA AGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACATTATTGT GAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCAAGCCGGG AATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCGCTATAAC TATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTACATGTCGT GGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATATCCAGGC GCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGCAGAGATT TCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAGAAAGGGG AATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTTGAGCGGA TGGGTGGAGGGTCTCGTGACCACATGGTCCTTCATGAGTACGTAAATGCT GCTGGGATTACAGGTGGCGGATCACTCGAAGTTATGGCAGAAGTTCGGCC CATAGCCCGATCCATTAAAACGGCTCACGACGATGCGCGAGCGGAATTAA TGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATGATTCTAGAAGAGA CTCTTATCAAGAATCCCATCTCTTGCTTGCTTTTTTTTGTTGCTTCCCTT TGATAGGGTTTGTTTTTCTTGTTTCAGTGACTTTCTATGTTAAAAGATAA TGTCAGTAAAAGGATTTGGTTTTCTATTATTCTGAATCGATTACGGAAGA TTCTTGCTTAATTCCAATCTATACAAGTATCGTGAAATAATGACCGTTTA TGTAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACC CTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGC TGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG CAGCCTGAATGGCGAATGCTAGAGCAGCTTGAGCTTGGATCAGATTGTCG TTTCCCGCCTTCAGTTTAAACTATCAGTGTTTGACAGGATATATTGGCGG GTAAACCTAAGAGAAAAGAGCGTTTATTAGAATAACGGATATTTAAAAGG GCGTGAAAAGGTTTATCCGTTCGTCCATTTGTATGTGCATGCCAACCACA GGGTTCCCCTCGGGATCAA

> SEQ ID NO 21 Deleted virD2 sequence in XYA105DvirD2

atgcccgatcgcgctcaagtaatcattcgcattgtgccaggaggtggaac caagacccttcagcagataatcaatcagctggagtacctgtcccgaaagg gaaagctggaactgcagcgttcagcccggcatctcgatattcccgttccg ccggatcaaatccgtgagcttgcccaaagctgggttacggaggccgggat ttatgacgaaagtcagtcagacgatgacaggcaacaagacttaacaacac acattattgtaagcttccccgcaggtaccgaccaaaccgcagcttatgaa gcaagccgggaatgggcagccgagatgtttgggtcaggatacgggggtgg ccgctataactatctgacagcctaccacgtcgaccgcgatcatccacatt tacatgtcgtggtcaatcgtcgggaacttctggggcaggggtggctgaaa atatccaggcgccatccccagctgaattatgacggcttacggaaaaagat ggcagagatttcacttcgtcacggcatagtcctggatgcgacttcgcgag cagaaaggggaatagcagagcgaccaatcacatatgctgaatatcgacgc cttgagcggatgcaggctcaaaagattcaattcgaagatacagattttga tgagacctcgcctgaggaagatcgtcgggacctcagtcaatcgttcgatc catttcgatcggacgcatctgccggcgaaccggaccgtgcaacccgacat gacaaacaaccgcttgaaccgcacgcccgtttccaggagcccgccggctc cagcatcaaagccgacgcacggatccgcgtaccattggagagcgagcggg gtgcccaaccatccgcgtccaaaatccctgtaactgggcatttcgggatt gagacttcgtatgtcgctgaagccagcgtgcccaaacaaagcggcaattc cgatacttctcgcccggtgactgacgttgccatgcacacagtcgagcgcc agcagcgatcaaaacgacgtcatgacgaggaggcaggtccgagcggagca aaccgtaaaagattgaaggccgcgcaagttgattccgaggcaaatgtcgg tgagcccgacggtcgcgatgacagcaacaaggcggctgatccggtgtctg cttccatccgtaccgagcaaccggaagcttctccaacgtgtccgcgtgac cgtcacgatggagaattgggagaacgcaaacgtgcaagaggtaatcgtcg cgacgatgggcgcggggggacctag

> SEQ ID NO 22 DNA sequence of backbone vector pAGa

actttgatccaacccctccgctgctatagtgcagtcggcttctgacgttc agtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgc gacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgtttt agtcgcataaagtagaatacttgcgactagaaccggagacattacgccat gaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccg acgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgc accaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccgga gctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtga ccaggctagaccgcctggcccgcagcacccgcgacctactggacattgcc gagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtg ggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccg gcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcggg cgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccct caccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggcc gcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctg taccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcg gcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcgg ccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacg gccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatc gcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggct gcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccgg ccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgt gtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcg atgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgt acttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctag cccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgat ccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgct aaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggcca tcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggac ttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgca gccaagcccttacgacatatgggccaccgccgacctggtggagctggtta agcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtg tcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgct ggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtga gctacccaggcactgccgccgccggcacaaccgttcttgaatcagaaccc gagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatc aaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaa cacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgt tggccagcctggcagacacgccagccatgaagcgggtcaactttcagttg ccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaa gaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaa tgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcgg catggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgt gtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccgg ccctgcaatggcactggaacccccaagcccgaggaatcggcgtgagcggt cgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacct ggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgagg cagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgc aaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagcc gcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacg tgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctg tcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccaga cgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtggg attacgacctggtactgatggcggtttcccatctaaccgaatccatgaac cgataccgggaagggaagggagacaagcccggccgcgtgttccgtccaca cgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcaga aagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgcc atgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccga gggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggc ggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatc acagaaggcaagaacccggacgtgctgacggttcaccccgattacttttt gatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccg caggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagt ggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgat cgggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcagg ctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagca tccgccggttcctaatgtacggagcagatgctagggcaaattgccctagc aggggaaaaaggtcgaaaaggtctctttcctgtggatagcacgtacattg ggaacccaaagccgtacattgggaaccggaacccgtacattgggaaccca aagccgtacattgggaaccggtcacacatgtaagtgactgatataaaaga gaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactc ttaaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaa gagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgc cgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcc tacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactc gaccgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatg acggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgt ctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcggg tgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcg gagtgtatactggcttaactatgcggcatcagagcagattgtactgagag tgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatac cgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggt cgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggt tatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttcca taggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga ggtggcgaaacccgacaggactataaagataccaggcgtttccccctgga agctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacct gtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgct gtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcg tcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttc ttgaagtggtggcctaactacggctacactagaaggacagtatttggtat ctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctctt gatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatctt ttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt tggtcatgcatGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTC TTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA AGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT TTTGGTCATGAGCTTGCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCCA GTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCA AATGAAACTGCAATTTATTCACATCAGGATTATCAATACCATATTTTTGA AAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAG GATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAA TACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAA TCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTgTGCAT TTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAAT CACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCAAGA CGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATG CAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAAT CAGGATATTCTTCTAATACCTGGAATGCTGTTTTTCCGGGGATCGCAGTG GTGAGTAACCAcGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGG AAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAA CATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCA TCGGGCTTCCCATACAAGCGATAGATTGTCGCACCTGATTGCCCGACATT ATCGCGtGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTA ATCGCGGCCTCGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTA TTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTT ATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTCCC CCCCCCCCCCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCag gcctcTGGCAGGATATATTGTGGTGTAAACCGAGCTGTaTaGGCTGGCTG GTGGCAGGATATATTGTGGTGTAAACttaattaaTAGGGATAACAGGGTA ATGGCGCGCCtgacaggatatattggcgggtaaactaagtcgctgtatgt gtttgtttggaattcgagctcggtacccggggatcctctagagtcgacct gcaggcatgcaagcttagt

> SEQ ID NO 23 DNA sequence of backbone vector pAGb

actttgatccaacccctccgctgctatagtgcagtcggcttctgacgttc agtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgc gacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgtttt agtcgcataaagtagaatacttgcgactagaaccggagacattacgccat gaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccg acgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgc accaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccgga gctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtga ccaggctagaccgcctggcccgcagcacccgcgacctactggacattgcc gagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtg ggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccg gcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcggg cgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccct caccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggcc gcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctg taccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcg gcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcgg ccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacg gccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatc gcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggct gcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccgg ccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgt gtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcg atgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgt acttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctag cccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgat ccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgct aaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggcca tcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggac ttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgca gccaagcccttacgacatatgggccaccgccgacctggtggagctggtta agcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtg tcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgct ggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtga gctacccaggcactgccgccgccggcacaaccgttcttgaatcagaaccc gagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatc aaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaa cacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgt tggccagcctggcagacacgccagccatgaagcgggtcaactttcagttg ccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaa gaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaa tgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcgg catggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgt gtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccgg ccctgcaatggcactggaacccccaagcccgaggaatcggcgtgagcggt cgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacct ggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgagg cagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgc aaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagcc gcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacg tgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctg tcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccaga cgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtggg attacgacctggtactgatggcggtttcccatctaaccgaatccatgaac cgataccgggaagggaagggagacaagcccggccgcgtgttccgtccaca cgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcaga aagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgcc atgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccga gggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggc ggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatc acagaaggcaagaacccggacgtgctgacggttcaccccgattacttttt gatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccg caggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagt ggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgat cgggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcagg ctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagca tccgccggttcctaatgtacggagcagatgctagggcaaattgccctagc aggggaaaaaggtcgaaaaggtctctttcctgtggatagcacgtacattg ggaacccaaagccgtacattgggaaccggaacccgtacattgggaaccca aagccgtacattgggaaccggtcacacatgtaagtgactgatataaaaga gaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactc ttaaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaa gagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgc cgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcc tacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactc gaccgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatg acggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgt ctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcggg tgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcg gagtgtatactggcttaactatgcggcatcagagcagattgtactgagag tgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatac cgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggt cgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggt tatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttcca taggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga ggtggcgaaacccgacaggactataaagataccaggcgtttccccctgga agctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacct gtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgct gtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcg tcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttc ttgaagtggtggcctaactacggctacactagaaggacagtatttggtat ctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctctt gatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatctt ttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt tggtcatgcatGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTC TTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA AGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT TTTGGTCATGAGCTTGCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCCA GTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCA AATGAAACTGCAATTTATTCACATCAGGATTATCAATACCATATTTTTGA AAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAG GATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAA TACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAA TCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTgTGCAT TTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAAT CACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCAAGA CGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATG CAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAAT CAGGATATTCTTCTAATACCTGGAATGCTGTTTTTCCGGGGATCGCAGTG GTGAGTAACCAcGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGG AAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAA CATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCA TCGGGCTTCCCATACAAGCGATAGATTGTCGCACCTGATTGCCCGACATT ATCGCGtGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTA ATCGCGGCCTCGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTA TTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTT ATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTCCC CCCCCCCCCCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCag gcctcTGGCAGGATATATTGTGGTGTAAACCGAGCTGTaTaGGCTGGCTG GTGGCAGGATATATTGTGGTGTAAACttaattaaTAGGGATAACAGGGTA ATGGCGCGCCtgacaggatatattggcgggtaaactaagtcgctgtatgt gtttgtttgaagcttgcatgcctgcaggtcgactctagaggatccccggg taccgagctcgaattcagt

> SEQ ID NO 24 DNA sequence of backbone vector pAGc

actttgatccaacccctccgctgctatagtgcagtcggcttctgacgttc agtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgc gacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgtttt agtcgcataaagtagaatacttgcgactagaaccggagacattacgccat gaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccg acgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgc accaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccgga gctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtga ccaggctagaccgcctggcccgcagcacccgcgacctactggacattgcc gagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtg ggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccg gcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcggg cgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccct caccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggcc gcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctg taccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcg gcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcgg ccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacg gccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatc gcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggct gcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccgg ccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgt gtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcg atgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgt acttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctag cccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgat ccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgct aaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggcca tcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggac ttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgca gccaagcccttacgacatatgggccaccgccgacctggtggagctggtta agcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtg tcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgct ggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtga gctacccaggcactgccgccgccggcacaaccgttcttgaatcagaaccc gagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatc aaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaa cacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgt tggccagcctggcagacacgccagccatgaagcgggtcaactttcagttg ccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaa gaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaa tgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcgg catggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgt gtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccgg ccctgcaatggcactggaacccccaagcccgaggaatcggcgtgagcggt cgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacct ggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgagg cagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgc aaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagcc gcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacg tgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctg tcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccaga cgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtggg attacgacctggtactgatggcggtttcccatctaaccgaatccatgaac cgataccgggaagggaagggagacaagcccggccgcgtgttccgtccaca cgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcaga aagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgcc atgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccga gggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggc ggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatc acagaaggcaagaacccggacgtgctgacggttcaccccgattacttttt gatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccg caggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagt ggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgat cgggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcagg ctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagca tccgccggttcctaatgtacggagcagatgctagggcaaattgccctagc aggggaaaaaggtcgaaaaggtctctttcctgtggatagcacgtacattg ggaacccaaagccgtacattgggaaccggaacccgtacattgggaaccca aagccgtacattgggaaccggtcacacatgtaagtgactgatataaaaga gaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactc ttaaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaa gagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgc cgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcc tacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactc gaccgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatg acggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgt ctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcggg tgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcg gagtgtatactggcttaactatgcggcatcagagcagattgtactgagag tgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatac cgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggt cgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggt tatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttcca taggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga ggtggcgaaacccgacaggactataaagataccaggcgtttccccctgga agctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacct gtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgct gtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcg tcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttc ttgaagtggtggcctaactacggctacactagaaggacagtatttggtat ctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctctt gatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatctt ttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt tggtcatgcatGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTC TTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA AGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT TTTGGTCATGAGCTTGCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCCA GTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCA AATGAAACTGCAATTTATTCACATCAGGATTATCAATACCATATTTTTGA AAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAG GATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAA TACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAA TCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTgTGCAT TTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAAT CACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCAAGA CGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATG CAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAAT CAGGATATTCTTCTAATACCTGGAATGCTGTTTTTCCGGGGATCGCAGTG GTGAGTAACCAcGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGG AAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAA CATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCA TCGGGCTTCCCATACAAGCGATAGATTGTCGCACCTGATTGCCCGACATT ATCGCGtGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTA ATCGCGGCCTCGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTA TTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTT ATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTCCC CCCCCCCCCCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCag gcctgaattcgagctcggtacccggggatcctctagagtcgacctgcagg catgcaagcttcTGGCAGGATATATTGTGGTGTAAACCGAGCTGTaTaGG CTGGCTGGTGGCAGGATATATTGTGGTGTAAACttaattaaTAGGGATAA CAGGGTAATGGCGCGCCtgacaggatatattggcgggtaaactaagtcgc tgtatgtgtttgtttgagtact

> SEQ ID NO 25 DNA sequence of backbone vector pAGd

actttgatccaacccctccgctgctatagtgcagtcggcttctgacgttc agtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgc gacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgtttt agtcgcataaagtagaatacttgcgactagaaccggagacattacgccat gaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccg acgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgc accaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccgga gctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtga ccaggctagaccgcctggcccgcagcacccgcgacctactggacattgcc gagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtg ggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccg gcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcggg cgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccct caccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggcc gcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctg taccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcg gcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcgg ccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacg gccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatc gcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggct gcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccgg ccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgt gtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcg atgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgt acttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctag cccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgat ccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgct aaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggcca tcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggac ttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgca gccaagcccttacgacatatgggccaccgccgacctggtggagctggtta agcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtg tcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgct ggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtga gctacccaggcactgccgccgccggcacaaccgttcttgaatcagaaccc gagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatc aaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaa cacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgt tggccagcctggcagacacgccagccatgaagcgggtcaactttcagttg ccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaa gaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaa tgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcgg catggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgt gtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccgg ccctgcaatggcactggaacccccaagcccgaggaatcggcgtgagcggt cgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacct ggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgagg cagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgc aaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagcc gcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacg tgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctg tcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccaga cgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtggg attacgacctggtactgatggcggtttcccatctaaccgaatccatgaac cgataccgggaagggaagggagacaagcccggccgcgtgttccgtccaca cgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcaga aagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgcc atgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccga gggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggc ggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatc acagaaggcaagaacccggacgtgctgacggttcaccccgattacttttt gatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccg caggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagt ggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgat cgggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcagg ctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagca tccgccggttcctaatgtacggagcagatgctagggcaaattgccctagc aggggaaaaaggtcgaaaaggtctctttcctgtggatagcacgtacattg ggaacccaaagccgtacattgggaaccggaacccgtacattgggaaccca aagccgtacattgggaaccggtcacacatgtaagtgactgatataaaaga gaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactc ttaaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaa gagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgc cgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcc tacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactc gaccgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatg acggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgt ctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcggg tgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcg gagtgtatactggcttaactatgcggcatcagagcagattgtactgagag tgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatac cgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggt cgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggt tatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttcca taggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga ggtggcgaaacccgacaggactataaagataccaggcgtttccccctgga agctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacct gtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgct gtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcg tcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttc ttgaagtggtggcctaactacggctacactagaaggacagtatttggtat ctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctctt gatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatctt ttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt tggtcatgcatGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTC TTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA AGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT TTTGGTCATGAGCTTGCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCCA GTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCA AATGAAACTGCAATTTATTCACATCAGGATTATCAATACCATATTTTTGA AAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAG GATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAA TACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAA TCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTgTGCAT TTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAAT CACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCAAGA CGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATG CAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAAT CAGGATATTCTTCTAATACCTGGAATGCTGTTTTTCCGGGGATCGCAGTG GTGAGTAACCAcGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGG AAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAA CATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCA TCGGGCTTCCCATACAAGCGATAGATTGTCGCACCTGATTGCCCGACATT ATCGCGtGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTA ATCGCGGCCTCGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTA TTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTT ATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTCCC CCCCCCCCCCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCag gcctaagcttgcatgcctgcaggtcgactctagaggatccccgggtaccg agctcgaattccTGGCAGGATATATTGTGGTGTAAACCGAGCTGTaTaGG CTGGCTGGTGGCAGGATATATTGTGGTGTAAACttaattaaTAGGGATAA CAGGGTAATGGCGCGCCtgacaggatatattggcgggtaaactaagtcgc tgtatgtgtttgtttgagtact

>SEQ ID NO 26 DNA sequence of binary chloroplast transformation vector pAG-CpTEST_ Note dicistronic aadA-gfp marker genes

actttgatccaacccctccgctgctatagtgcagtcggcttctgacgttc agtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgc gacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgtttt agtcgcataaagtagaatacttgcgactagaaccggagacattacgccat gaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccg acgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgc accaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccgga gctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtga ccaggctagaccgcctggcccgcagcacccgcgacctactggacattgcc gagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtg ggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccg gcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcggg cgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccct caccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggcc gcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctg taccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcg gcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcgg ccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacg gccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatc gcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggct gcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccgg ccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgt gtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcg atgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgt acttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctag cccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgat ccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgct aaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggcca tcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggac ttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgca gccaagcccttacgacatatgggccaccgccgacctggtggagctggtta agcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtg tcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgct ggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtga gctacccaggcactgccgccgccggcacaaccgttcttgaatcagaaccc gagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatc aaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaa cacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgt tggccagcctggcagacacgccagccatgaagcgggtcaactttcagttg ccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaa gaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaa tgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcgg catggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgt gtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccgg ccctgcaatggcactggaacccccaagcccgaggaatcggcgtgagcggt cgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacct ggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgagg cagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgc aaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagcc gcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacg tgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctg tcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccaga cgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtggg attacgacctggtactgatggcggtttcccatctaaccgaatccatgaac cgataccgggaagggaagggagacaagcccggccgcgtgttccgtccaca cgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcaga aagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgcc atgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccga gggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggc ggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatc acagaaggcaagaacccggacgtgctgacggttcaccccgattacttttt gatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccg caggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagt ggcagcgccggagagttcaagaagttctgtttcaccgtgcgcaagctgat cgggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcagg ctggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagca tccgccggttcctaatgtacggagcagatgctagggcaaattgccctagc aggggaaaaaggtcgaaaaggtctctttcctgtggatagcacgtacattg ggaacccaaagccgtacattgggaaccggaacccgtacattgggaaccca aagccgtacattgggaaccggtcacacatgtaagtgactgatataaaaga gaaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactc ttaaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaa gagctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgc cgcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcc tacggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactc gaccgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatg acggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgt ctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcggg tgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcg gagtgtatactggcttaactatgcggcatcagagcagattgtactgagag tgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatac cgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggt cgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggt tatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttcca taggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga ggtggcgaaacccgacaggactataaagataccaggcgtttccccctgga agctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacct gtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgct gtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcg tcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttc ttgaagtggtggcctaactacggctacactagaaggacagtatttggtat ctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctctt gatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatctt ttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt tggtcatgcatGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTC TTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA AGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT TTTGGTCATGAGCTTGCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCCA GTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCA AATGAAACTGCAATTTATTCACATCAGGATTATCAATACCATATTTTTGA AAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAG GATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAA TACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAA TCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTgTGCAT TTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAAT CACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCAAGA CGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATG CAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAAT CAGGATATTCTTCTAATACCTGGAATGCTGTTTTTCCGGGGATCGCAGTG GTGAGTAACCAcGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGG AAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAA CATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCA TCGGGCTTCCCATACAAGCGATAGATTGTCGCACCTGATTGCCCGACATT ATCGCGtGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTA ATCGCGGCCTCGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTA TTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTT ATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTCCC CCCCCCCCCCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCag gcctcTGGCAGGATATATTGTGGTGTAAACCGAGCTGTaTaGGCTGGCTG GTGGCAGGATATATTGTGGTGTAAACttaattaaAATTCACCGCCGTATG GCTGACCGGCGATTACTAGCGATTCCGGCTTCATGCAGGCGAGTTGCAGC CTGCAATCCGAACTGAGGACGGGTTTTTGGGGTTAGCTCACCCTCGCGGG ATCGCGACCCTTTGTCCCGGCCATTGTAGCACGTGTGTCGCCCAGGGCAT AAGGGGCATGATGACTTGACGTCATCCTCACCTTCCTCCGGCTTATCACC GGCAGTCTGTTCAGGGTTCCAAACTCAACGATGGCAACTAAACACGAGGG TTGCGCTCGTTGCGGGACTTAACCCAACACCTTACGGCACGAGCTGACGA CAGCCATGCACCACCTGTGTCCGCGTTCCCGAAGGCACCCCTCTCTTTCA AGAGGATTCGCGGCATGTCAAGCCCTGGTAAGGTTCTTCGCTTTGCATCG AATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTG AGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTAACGCGTTAG CTACAGCACTGCACGGGTCGATACGCACAGCGCCTAGTATCCATCGTTTA CGGCTAGGACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGT CTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCT TTCCGATCTCTACGCATTTCACCGCTCCACCGGAAATTCCCTCTGCCCCT ACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGCCCTG GGATTTGACGGCGGACTTAAAAAGCCACCTACAGACGCTTTACGCCCAAT CATTCCGGATAACGCTTGCATCCTCTGTATTACCGCGGCTGCTGGCACAG AGTTAGCCGATGCTTATTCCCCAGATACCGTCATTGCTTCTTCTCCGGGA AAAGAAGTTCACGACCCGTGGGCCTTCTACCTCCACGCGGCATTGCTCCG TCAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTGCCTCCCGTAGGA GTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCGGACCAG CTACTGATCATCGCCTTGGTAAGCTATTGCCTCACCAACTAGCTAATCAG ACGCGAGCCCCTCCTCGGGCGGATTCCTCCTTTTGCTCCTCAGCCTACGG GGTATTAGCAGCCGTTTCCAGCTGTTGTTCCCCTCCCAAGGGCAGGTTCT TACGCGTTACTCACCCGTCCGCCACTGGAAACACCACTTCCCGTCCGACT TGCATGTGTTAAGCATGCCGCCAGCGTTCATCCTGAGCCAGGATCGAACT CTCCATGAGATTCATAGTTGCATTACTTATAGCTTCCTTGTTCGTAGACA AAGCGGATTCGGAATTGTCTTTCATTCCAAGGCATAACTTGTATCCATGC GCTTCATATTCGCCCGGAGTTCGCTCCCAGAAATATAGCCATCCCTGCCC CCTCACGTCAATCCCACGAGCCTCTTATCCATTCTCATTGAACGACGGCG GGGGAGCAAATCCAACTAGAAAAACTCACATTGGGCTTAGGGATAATCAG GCTCGAACTGATGACTTCCACCACGTCAAGGTGACACTCTACCGCTGAGT TATATCCCTTCCCCGCCCCATCGAGAAATAGAACTGACTAATCCTAAGTC AAAGGGTCGAGAAACTCAACGCCACTATTCTTGAACAACTTGGAGCCGGG CCTTCTTTTCGCACTATTACGGATATGAAAATAATGGTCAAAATCGGATT CAATTGTCTACcGCTCCCCCGCCGTCGTTCAATGAGAATGGATAAGAGGC TCGTGGGATTGACGTGAGGGGGCAGGGATGGCTATATTTCTGGGAGAATT AACCGATCGACGTGCaAGCGGACATTTATTTTaAATTCGATAATTTTTGC AAAAACATTTCGACATATTTATTTATTTTATTATTATGAGAATCAATCCT ACTACTTCTGGTTCTGGGGTTTCCACGgctactagcGAAGCGGTGATCGC CGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATC TCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGC GGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAG GCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTT CGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATT GTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACT GCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGC CAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAA CATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGT TCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGA ACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTG TCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGT CGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCA TACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCC TCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGAT CACCAAGGTAGTgGGCAAAgaaCAAAAACTCATTTCTGAAGAAGACTTGT AACTGCAGATAACCCAAATAATGTTTTAAAATTTTAAAAATAATGTAGGA GGAAAAATTATGgctagcAGTAAAGGAGAAGAACTTTTCACTGGAGTTGT CCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTG TCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAA TTTATTTGCACTACTGGAAAACTACCTGTTCCtTGGCCAACACTTGTCAC TACTTTCTCTTATGGTGTTCAATGCTTTTCAAGATACCCAGATCATATGA AGCGGCACGACTTCTTCAAGAGCGCCATGCCTGAGGGATACGTGCAGGAG AGGACCATCTCTTTCAAGGACGACGGGAACTACAAGACACGTGCTGAAGT CAAGTTTGAGGGAGACACCCTCGTCAACAGGATCGAGCTTAAGGGAATCG ATTTCAAGGAGGACGGAAACATCCTCGGCCACAAGTTGGAATACAACTAC AACTCCCACAACGTATACATCACGGCAGACAAACAAAAGAATGGAATCAA AGCTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAG CAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTA CCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAA CGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGA TTACACATGGCATGGATGAACTATACAAATAAGctctagCTAGAGCgatc ctggcctagtctataggaggttttgaaaagaaaggagcaataatcatttt cttgttctatcaagagggtgctattgctcctttctttttttctttttatt tatttactagtattttacttacatagacttttttgtttacattatagaaa aagaaggagaggttattttcttgcatttattcatgGGGGATCAAAGCTAA CAACTGCCCCTATCGGAAATAGGATTGACTACCGATTCCGAAGGAACTGG AGTTACATCTCTTTTCCATTCAAGAGTTCTTATGCGTTTCCACGCCCCTT TGAGACCCCGAAAAATGGACAAATTCCTTTTCTTAGGAACACATACAAGA TTCGTCACTACAAAAAGGATAATGGTAACCCTACCATTAACTACTTCATT TATGAATTTCATAGTAATAGAAATACATGTCCTACCGAGACAGAATTTGG AACTTGCTATCCTCTTGCCTAGCAGGCAAAGATTTACCTCCGTGGAAAGG ATGATTCATTCGGATCGACATGAGAGTCCAACTACATTGCCAGAATCCAT GTTGTATATTTGAAAGAGGTTGACCTCCTTGCTTCTCTCATGGTACACTC CTCTTCCCGCCGAGCCCCTTTTCTCCTCGGTCCACAGAGACAAAATGTAG GACTGGTGCCAACAATTCATCAGACTCACTAAGTCGGGATCACTAACTAA TACTAATCTAATATAATAGTCTAATATATCTAATATAATAGAAAATACTA ATATAATAGAAAAGAACTGTCTTTTCTGTATACTTTCCCCGGTTCCGTTG CTACCGCGGGCTTTACGCAATCGATCGGATTAGATAGATATCCCTTCAAC ATAGGTCATCGAAAGGATCTCGGAGACCCACCAAAGTACGAAAGCCAGGA TCTTTCAGAAAACGGATTCCTATTCAAAGAGTGCATAACCGCATGGATAA GCTCACACTAACCCGTCAATTTGGGATCCAAATTCGAGATTTTCCTTGGG AGGTATCGGGAAGGATTTGGAATGGAATAATATCGATTCATACAGAAGAA AAGGTTCTCTATTGATTCAAACACTGTACCTAACCTATGGGATAGGGATC GAGGAAGGGGAAAAACCGAAGATTTCACATGGTACTTTTATCAATCTGAT TTATTTCGTACCTTTCGTTCAATGAGAAAATGGGTCAAATTCTACAGGAT CAAACCTATGGGACTTAAGGAATGATATAAAAAAAAGAGAGGGAAAATAT TCATATTAAATAAATATGAAGTAGAAGAACCCAGATTCCAAATGAACAAA TTCAAACTTGAAAAGGATCTTCCTTATTCTTGAAGAATGAGGGGCAAAGG GATTGATCAAGAAAGATCGGCGCGCCtgacaggatatattggcgggtaaa ctaagtcgctgtatgtgtttgtttggaattcgagctcggtacccggggat cctctagagtcgacctgcaggcatgcaagcttagt

> SEQ ID NO 27 The DNA sequence of Pt-VirD2 in a VirD promoter/VirF terminator cassette. The Pt-VirD2 has the tobacco SSU Transit peptide+5aa, the mutant minimal VirD2-T4SS signal.

ttatcgatgagtaaaagcgttgttacactatttttatttcacattcgtta taagacaattgcaaatgtagcaagtatattcagtattgactgtaaatgta ctgttgatttcatattgagcagggctagacttccatccgtctacccgggc acatttcgtgctggagtatccagaccttccgctttctttggaggaagcta tggcttcctcagttctttcctccgcagcagttgccacccgcagcaatgtt gctcaagctaacatggttgcacctttcactggccttaagtcagctgcctc attccctgtttcaaggaagcaaaaccttgacatcacttccattgccagca acggcggaagagtgcaatgcatgCAGGTGTGGCCAGCGATGGCTCCCGAT CGGGCCcaagtaatcattcgcattgtgccaggaggtggaaccaagaccct tcagcagataatcaatcagctggagtacctgtccacgaagggaaagctgg aactccagcgttcagcccggcatctcgatattcccgttccgccggatcaa atccgtgagcttgcccaaagctgggttacggaggccgggatttatgacga aagtcagtcagacgatgacaggcaacaagacttaacaacacacattattg tgagcttccccgcaggtacggaccaaaccgcagcttatgaagcaagccgg gaatgggcagccgagatgtttgggtcaggatacgggggtggccgctataa ctatctgacagcctaccacgtggaccgcgatcatccacatttacatgtcg tggtcaatcgtcgggaacttctggggcaggggtggctgaaaatatccagg cgccatccccagctgaattatgacggcttacggaaaaagatggcagagat ttcacttcgtcacggcatagtcctggatgcgacttcccgagcagaaaggg gaatagcagagcgaccaatcacctatgctgaatatcgacgtCTTGAGCGG ATGCTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCATTAAAAC GGCTCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGACCTCGAT CAACGCGCGGTCTATGATTctagaatccgtgctgttcgtcacccgcttat agttgccatcacaatcggatttcaactgccccggtctttaggtgcggccg aggacaatgtagcacgcacttacggtgcgtctcgtctctgacgctttcta tgttccgca

> SEQ ID NO 28 The DNA sequence of Pt-VirD2 in a VirE promoter/VirF terminator cassette. The Pt-VirD2 has the tobacco SSU Transit peptide+5aa, the mutant minimal VirD2-T4SS signal.

cggctgctcgtcaccaacaatgctcacagggatacgatggcctcgacagt caggccgcgtgcgtccagtctttccagttcctccctttcaggtcgattgt ggcatcatttattgcctgctcattgcagttgaaacgcgatatccgtttca agacccgagtatggatggtactttggagatatgagcacttgccagacttc ccggtcggagacttaaaaattccgataagaggttactgacgacggacctt ataagtgggtcgtctagtggtcgcactgatcaaaacagttccgcccccgg ctttgctcaaagtagcaaagcagctttatctcgggttgcagaggattttc tggaaaaccgcaattttgcaggagagaacatatggcttcctcagttcttt cctccgcagcagttgccacccgcagcaatgttgctcaagctaacatggtt gcacctttcactggccttaagtcagctgcctcattccctgtttcaaggaa gcaaaaccttgacatcacttccattgccagcaacggcggaagagtgcaat gcatgCAGGTGTGGCCAGCGATGGCTCCCGATCGGGCCcaagtaatcatt cgcattgtgccaggaggtggaaccaagacccttcagcagataatcaatca gctggagtacctgtccacgaagggaaagctggaactccagcgttcagccc ggcatctcgatattcccgttccgccggatcaaatccgtgagcttgcccaa agctgggttacggaggccgggatttatgacgaaagtcagtcagacgatga caggcaacaagacttaacaacacacattattgtgagcttccccgcaggta cggaccaaaccgcagcttatgaagcaagccgggaatgggcagccgagatg tttgggtcaggatacgggggtggccgctataactatctgacagcctacca cgtggaccgcgatcatccacatttacatgtcgtggtcaatcgtcgggaac ttctggggcaggggtggctgaaaatatccaggcgccatccccagctgaat tatgacggcttacggaaaaagatggcagagatttcacttcgtcacggcat agtcctggatgcgacttcccgagcagaaaggggaatagcagagcgaccaa tcacctatgctgaatatcgacgtCTTGAGCGGATGCTCGAAGTTATGGCA GAAGTTCGGCCCATAGCCCGATCCATTAAAACGGCTCACGACGATGCGCG AGCGGAATTAATGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATGAT Tctagaatccgtgctgttcgtcacccgcttatagttgccatcacaatcgg atttcaactgccccggtctttaggtgcggccgaggacaatgtagcacgca cttacggtgcgtctcgtctctgacgctttctatgttccgca

> SEQ ID NO 29 DNA sequence of pAG-CHL-1 chloroplast binary transformation vector for introduction of transgenes (goi) into the plastid genome. The binary vector carries a Pt-VirD2 gene outside the T-DNA listed as SEQ ID No 27.

actttgatccaacccctccgctgctatagtgcagtcggcttctgacgttc agtgcagccgtcttctgaaaacgacatgtcgcacaagtcctaagttacgc gacaggctgccgccctgcccttttcctggcgttttcttgtcgcgtgtttt agtcgcataaagtagaatacttgcgactagaaccggagacattacgccat gaacaagagcgccgccgctggcctgctgggctatgcccgcgtcagcaccg acgaccaggacttgaccaaccaacgggccgaactgcacgcggccggctgc accaagctgttttccgagaagatcaccggcaccaggcgcgaccgcccgga gctggccaggatgcttgaccacctacgccctggcgacgttgtgacagtga ccaggctagaccgcctggcccgcagcacccgcgacctactggacattgcc gagcgcatccaggaggccggcgcgggcctgcgtagcctggcagagccgtg ggccgacaccaccacgccggccggccgcatggtgttgaccgtgttcgccg gcattgccgagttcgagcgttccctaatcatcgaccgcacccggagcggg cgcgaggccgccaaggcccgaggcgtgaagtttggcccccgccctaccct caccccggcacagatcgcgcacgcccgcgagctgatcgaccaggaaggcc gcaccgtgaaagaggcggctgcactgcttggcgtgcatcgctcgaccctg taccgcgcacttgagcgcagcgaggaagtgacgcccaccgaggccaggcg gcgcggtgccttccgtgaggacgcattgaccgaggccgacgccctggcgg ccgccgagaatgaacgccaagaggaacaagcatgaaaccgcaccaggacg gccaggacgaaccgtttttcattaccgaagagatcgaggcggagatgatc gcggccgggtacgtgttcgagccgcccgcgcacgtctcaaccgtgcggct gcatgaaatcctggccggtttgtctgatgccaagctggcggcctggccgg ccagcttggccgctgaagaaaccgagcgccgccgtctaaaaaggtgatgt gtatttgagtaaaacagcttgcgtcatgcggtcgctgcgtatatgatgcg atgagtaaataaacaaatacgcaaggggaacgcatgaaggttatcgctgt acttaaccagaaaggcgggtcaggcaagacgaccatcgcaacccatctag cccgcgccctgcaactcgccggggccgatgttctgttagtcgattccgat ccccagggcagtgcccgcgattgggcggccgtgcgggaagatcaaccgct aaccgttgtcggcatcgaccgcccgacgattgaccgcgacgtgaaggcca tcggccggcgcgacttcgtagtgatcgacggagcgccccaggcggcggac ttggctgtgtccgcgatcaaggcagccgacttcgtgctgattccggtgca gccaagcccttacgacatatgggccaccgccgacctggtggagctggtta agcagcgcattgaggtcacggatggaaggctacaagcggcctttgtcgtg tcgcgggcgatcaaaggcacgcgcatcggcggtgaggttgccgaggcgct ggccgggtacgagctgcccattcttgagtcccgtatcacgcagcgcgtga gctacccaggcactgccgccgccggcacaaccgttcttgaatcagaaccc gagggcgacgctgcccgcgaggtccaggcgctggccgctgaaattaaatc aaaactcatttgagttaatgaggtaaagagaaaatgagcaaaagcacaaa cacgctaagtgccggccgtccgagcgcacgcagcagcaaggctgcaacgt tggccagcctggcagacacgccagccatgaagcgggtcaactttcagttg ccggcggaggatcacaccaagctgaagatgtacgcggtacgccaaggcaa gaccattaccgagctgctatctgaatacatcgcgcagctaccagagtaaa tgagcaaatgaataaatgagtagatgaattttagcggctaaaggaggcgg catggaaaatcaagaacaaccaggcaccgacgccgtggaatgccccatgt gtggaggaacgggcggttggccaggcgtaagcggctgggttgtctgccgg ccctgcaatggcactggaacccccaagcccgaggaatcggcgtgagcggt cgcaaaccatccggcccggtacaaatcggcgcggcgctgggtgatgacct ggtggagaagttgaaggccgcgcaggccgcccagcggcaacgcatcgagg cagaagcacgccccggtgaatcgtggcaagcggccgctgatcgaatccgc aaagaatcccggcaaccgccggcagccggtgcgccgtcgattaggaagcc gcccaagggcgacgagcaaccagattttttcgttccgatgctctatgacg tgggcacccgcgatagtcgcagcatcatggacgtggccgttttccgtctg tcgaagcgtgaccgacgagctggcgaggtgatccgctacgagcttccaga cgggcacgtagaggtttccgcagggccggccggcatggccagtgtgtggg attacgacctggtactgatggcggtttcccatctaaccgaatccatgaac cgataccgggaagggaagggagacaagcccggccgcgtgttccgtccaca cgttgcggacgtactcaagttctgccggcgagccgatggcggaaagcaga aagacgacctggtagaaacctgcattcggttaaacaccacgcacgttgcc atgcagcgtacgaagaaggccaagaacggccgcctggtgacggtatccga gggtgaagccttgattagccgctacaagatcgtaaagagcgaaaccgggc ggccggagtacatcgagatcgagctagctgattggatgtaccgcgagatc acagaaggcaagaacccggacgtgctgacggttcaccccgattacttttt gatcgatcccggcatcggccgttttctctaccgcctggcacgccgcgccg caggcaaggcagaagccagatggttgttcaagacgatctacgaacgcagt ggcagcgccggagagtcaagaagttctgtttcaccgtgcgcaagctgatc gggtcaaatgacctgccggagtacgatttgaaggaggaggcggggcaggc tggcccgatcctagtcatgcgctaccgcaacctgatcgagggcgaagcat ccgccggttcctaatgtacggagcagatgctagggcaaattgccctagca ggggaaaaaggtcgaaaaggtctctttcctgtggatagcacgtacattgg gaacccaaagccgtacattgggaaccggaacccgtacattgggaacccaa agccgtacattgggaaccggtcacacatgtaagtgactgatataaaagag aaaaaaggcgatttttccgcctaaaactctttaaaacttattaaaactct taaaacccgcctggcctgtgcataactgtctggccagcgcacagccgaag agctgcaaaaagcgcctacccttcggtcgctgcgctccctacgccccgcc gcttcgcgtcggcctatcgcggccgctggccgctcaaaaatggctggcct acggccaggcaatctaccagggcgcggacaagccgcgccgtcgccactcg accgccggcgcccacatcaaggcaccctgcctcgcgcgtttcggtgatga cggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtc tgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggt gttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcgg agtgtatactggcttaactatgcggcatcagagcagattgtactgagagt gcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatacc gcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtc gttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggtt atccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggcc agcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccat aggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagag gtggcgaaacccgacaggactataaagataccaggcgtttccccctggaa gctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctg tccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctg taggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgc acgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgt cttgagtccaacccggtaagacacgacttatcgccactggcagcagccac tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttct tgaagtggtggcctaactacggctacactagaaggacagtatttggtatc tgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttg atccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagc agcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttt tctacggggtctgacgctcagtggaacgaaaactcacgttaagggatttt ggtcatgcatGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCT TGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAA GCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCT TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATT TTGGTCATGAGCTTGCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCCAG TGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAA ATGAAACTGCAATTTATTCACATCAGGATTATCAATACCATATTTTTGAA AAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGG ATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAAT ACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAAT CACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTgTGCATT TCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATC ACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCAAGAC GAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGC AACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATC AGGATATTCTTCTAATACCTGGAATGCTGTTTTTCCGGGGATCGCAGTGG TGAGTAACCAcGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGA AGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAAC ATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCAT CGGGCTTCCCATACAAGCGATAGATTGTCGCACCTGATTGCCCGACATTA TCGCGtGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAA TCGCGGCCTCGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTAT TACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTA TCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTCCCC CCCCCCCCCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCagg cctcTGGCAGGATATATTGTGGTGTAAACCGAGCTGTaTaGGCTGGCTGG TGGCAGGATATATTGTGGTGTAAACttaattaaAATTCACCGCCGTATGG CTGACCGGCGATTACTAGCGATTCCGGCTTCATGCAGGCGAGTTGCAGCC TGCAATCCGAACTGAGGACGGGTTTTTGGGGTTAGCTCACCCTCGCGGGA TCGCGACCCTTTGTCCCGGCCATTGTAGCACGTGTGTCGCCCAGGGCATA AGGGGCATGATGACTTGACGTCATCCTCACCTTCCTCCGGCTTATCACCG GCAGTCTGTTCAGGGTTCCAAACTCAACGATGGCAACTAAACACGAGGGT TGCGCTCGTTGCGGGACTTAACCCAACACCTTACGGCACGAGCTGACGAC AGCCATGCACCACCTGTGTCCGCGTTCCCGAAGGCACCCCTCTCTTTCAA GAGGATTCGCGGCATGTCAAGCCCTGGTAAGGTTCTTCGCTTTGCATCGA ATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGA GTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTAACGCGTTAGC TACAGCACTGCACGGGTCGATACGCACAGCGCCTAGTATCCATCGTTTAC GGCTAGGACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTC TCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTT TCCGATCTCTACGCATTTCACCGCTCCACCGGAAATTCCCTCTGCCCCTA CCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGCCCTGG GATTTGACGGCGGACTTAAAAAGCCACCTACAGACGCTTTACGCCCAATC ATTCCGGATAACGCTTGCATCCTCTGTATTACCGCGGCTGCTGGCACAGA GTTAGCCGATGCTTATTCCCCAGATACCGTCATTGCTTCTTCTCCGGGAA AAGAAGTTCACGACCCGTGGGCCTTCTACCTCCACGCGGCATTGCTCCGT CAGGCTTTCGCCCATTGCGGAAAATTCCCCACTGCTGCCTCCCGTAGGAG TCTGGGCCGTGTCTCAGTCCCAGTGTGGCTGATCATCCTCTCGGACCAGC TACTGATCATCGCCTTGGTAAGCTATTGCCTCACCAACTAGCTAATCAGA CGCGAGCCCCTCCTCGGGCGGATTCCTCCTTTTGCTCCTCAGCCTACGGG GTATTAGCAGCCGTTTCCAGCTGTTGTTCCCCTCCCAAGGGCAGGTTCTT ACGCGTTACTCACCCGTCCGCCACTGGAAACACCACTTCCCGTCCGACTT GCATGTGTTAAGCATGCCGCCAGCGTTCATCCTGAGCCAGGATCGAACTC TCCATGAGATTCATAGTTGCATTACTTATAGCTTCCTTGTTCGTAGACAA AGCGGATTCGGAATTGTCTTTCATTCCAAGGCATAACTTGTATCCATGCG CTTCATATTCGCCCGGAGTTCGCTCCCAGAAATATAGCCATCCCTGCCCC CTCACGTCAATCCCACGAGCCTCTTATCCATTCTCATTGAACGACGGCGG GGGAGCAAATCCAACTAGAAAAACTCACATTGGGCTTAGGGATAATCAGG CTCGAACTGATGACTTCCACCACGTCAAGGTGACACTCTACCGCTGAGTT ATATCCCTTCCCCGCCCCATCGAGAAATAGAACTGACTAATCCTAAGTCA AAGGGTCGAGAAACTCAACGCCACTATTCTTGAACAACTTGGAGCCGGGC CTTCTTTTCGCACTATTACGGATATGAAAATAATGGTCAAAATCGGATTC AATTGTCTACcatgaataaatgcaagaaaataacctctccttctttttct ataatgtaaacaaaaaagtctatgtaagtaaaatactagtaaataaataa aaagaaaaaaagaaaggagcaatagcaccctcttgatagaacaagaaaat gattattgctcctttcttttcaaaacctcctatagactaggccaggatcT TACAAGTCTTCTTCAGAAATGAGTTTTTGttcTTTGCCcACTACCTTGGT GATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCG AGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGT ATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGC GACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGG ACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAG TTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGG AACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTAT GTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCT GGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTG CAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAA CAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCC GAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAG CCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGC CGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCG AGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTC GGCGATCACCGCTTCgctagtagcCGTGGAAACCCCAGAACCAGAAGTAG TAGGATTGATTCTCATAATAATAAAATAAATAAATATGTCGAAATGTTTT TGCAAAAATTATCGAATTtAAAATAAATGTCCGCTtGCACGTCGATCGGT TAATTCTCCCAGAAATATAGCCATCCCTGCCCCCTCACGTCAATCCCACG AGCCTCTTATCCATTCTCATTGAACGACGGCGGGGGAGCGTAgaattcga gctcggtacccggggatcctctagagtcgacctgcaggcatgcaagcttG TTAACAACTGCCCCTATCGGAAATAGGATTGACTACCGATTCCGAAGGAA CTGGAGTTACATCTCTTTTCCATTCAAGAGTTCTTATGCGTTTCCACGCC CCTTTGAGACCCCGAAAAATGGACAAATTCCTTTTCTTAGGAACACATAC AAGATTCGTCACTACAAAAAGGATAATGGTAACCCTACCATTAACTACTT CATTTATGAATTTCATAGTAATAGAAATACATGTCCTACCGAGACAGAAT TTGGAACTTGCTATCCTCTTGCCTAGCAGGCAAAGATTTACCTCCGTGGA AAGGATGATTCATTCGGATCGACATGAGAGTCCAACTACATTGCCAGAAT CCATGTTGTATATTTGAAAGAGGTTGACCTCCTTGCTTCTCTCATGGTAC ACTCCTCTTCCCGCCGAGCCCCTTTTCTCCTCGGTCCACAGAGACAAAAT GTAGGACTGGTGCCAACAATTCATCAGACTCACTAAGTCGGGATCACTAA CTAATACTAATCTAATATAATAGTCTAATATATCTAATATAATAGAAAAT ACTAATATAATAGAAAAGAACTGTCTTTTCTGTATACTTTCCCCGGTTCC GTTGCTACCGCGGGCTTTACGCAATCGATCGGATTAGATAGATATCCCTT CAACATAGGTCATCGAAAGGATCTCGGAGACCCACCAAAGTACGAAAGCC AGGATCTTTCAGAAAACGGATTCCTATTCAAAGAGTGCATAACCGCATGG ATAAGCTCACACTAACCCGTCAATTTGGGATCCAAATTCGAGATTTTCCT TGGGAGGTATCGGGAAGGATTTGGAATGGAATAATATCGATTCATACAGA AGAAAAGGTTCTCTATTGATTCAAACACTGTACCTAACCTATGGGATAGG GATCGAGGAAGGGGAAAAACCGAAGATTTCACATGGTACTTTTATCAATC TGATTTATTTCGTACCTTTCGTTCAATGAGAAAATGGGTCAAATTCTACA GGATCAAACCTATGGGACTTAAGGAATGATATAAAAAAAAGAGAGGGAAA ATATTCATATTAAATAAATATGAAGTAGAAGAACCCAGATTCCAAATGAA CAAATTCAAACTTGAAAAGGATCTTCCTTATTCTTGAAGAATGAGGGGCA AAGGGATTGATCAAGAAAGATCGGCGCGCCtgacaggatatattggcggg taaactaagtcgctgtatgtgtttgtttggaattttatcgatgagtaaaa gcgttgttacactatttttatttcacattcgttataagacaattgcaaat gtagcaagtatattcagtattgactgtaaatgtactgttgatttcatatt gagcagggctagacttccatccgtctacccgggcacatttcgtgctggag tatccagaccttccgctttctttggaggaagctatggcttcctcagttct ttcctccgcagcagttgccacccgcagcaatgttgctcaagctaacatgg ttgcacctttcactggccttaagtcagctgcctcattccctgtttcaagg aagcaaaaccttgacatcacttccattgccagcaacggcggaagagtgca atgcatgCAGGTGTGGCCAGCGATGGCTCCCGATCGGGCCcaagtaatca ttcgcattgtgccaggaggtggaaccaagacccttcagcagataatcaat cagctggagtacctgtccacgaagggaaagctggaactccagcgttcagc ccggcatctcgatattcccgttccgccggatcaaatccgtgagcttgccc aaagctgggttacggaggccgggatttatgacgaaagtcagtcagacgat gacaggcaacaagacttaacaacacacattattgtgagcttccccgcagg tacggaccaaaccgcagcttatgaagcaagccgggaatgggcagccgaga tgtttgggtcaggatacgggggtggccgctataactatctgacagcctac cacgtggaccgcgatcatccacatttacatgtcgtggtcaatcgtcggga acttctggggcaggggtggctgaaaatatccaggcgccatccccagctga attatgacggcttacggaaaaagatggcagagatttcacttcgtcacggc atagtcctggatgcgacttcccgagcagaaaggggaatagcagagcgacc aatcacctatgctgaatatcgacgtCTTGAGCGGATGCTCGAAGTTATGG CAGAAGTTCGGCCCATAGCCCGATCCATTAAAACGGCTCACGACGATGCG CGAGCGGAATTAATGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATG ATTctagaatccgtgctgttcgtcacccgcttatagttgccatcacaatc ggatttcaactgccccggtctttaggtgcggccgaggacaatgtagcacg cacttacggtgcgtctcgtctctgacgctttctatgttccgcagcttagt

> SEQ ID NO 30 DNA sequence of kanamycin resistance gene in StuI-NsiI fragment

aggcctGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGGGGGG GGGGGGGAAAGCCACGTTGTGTCTCAAAATCTCTGATGTTACATTGCACA AGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACA GTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCG CGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCaCG CGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGC CCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAAT GATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCC TCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCgTGGTTAC TCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATAT CCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCG GTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTAT TTCGTCTTGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCG AGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAA AGAAATGCAcAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATG GTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGT TGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGC CATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGC TTTTTCAAAAATATGGTATTGATAATCCTGATGTGAATAAATTGCAGTTT CATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTA ACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGAC CAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAG AAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGC TGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGA TCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCatgcat

> SEQ ID NO 31 The DNA sequence of Pt-VirD2-3 in a VirD promoter/VirF terminator cassette where Pt-VirD2 has the wild type NLS1. The Pt-VirD2-3 has the tobacco SSU Transit peptide+5aa, the wt minimal VirD2-T4SS signal.

ttatcgatgagtaaaagcgttgttacactatttttatttcacattcgtta taagacaattgcaaatgtagcaagtatattcagtattgactgtaaatgta ctgttgatttcatattgagcagggctagacttccatccgtctacccgggc acatttcgtgctggagtatccagaccttccgctttctttggaggaagcta tggcttcctcagttctttcctccgcagcagttgccacccgcagcaatgtt gctcaagctaacatggttgcacctttcactggccttaagtcagctgcctc attccctgtttcaaggaagcaaaaccttgacatcacttccattgccagca acggcggaagagtgcaatgcatgCAGGTGTGGCCAGCGATGGCTCCCGAT CGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAAGACCCT TCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAAAGCTGG AACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCGGATCAA ATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTATGACGA AAGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACATTATTG TGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCAAGCCGG GAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCGCTATAA CTATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTACATGTCG TGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATATCCAGG CGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGCAGAGAT TTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAGAAAGGG GAATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTTGAGCGG ATGCTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCATTAAAAC GGCTCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGACCTCGAT CAACGCGCGGTCTATGATTctagaatccgtgctgttcgtcacccgcttat agttgccatcacaatcggatttcaactgccccggtctttaggtgcggccg aggacaatgtagcacgcacttacggtgcgtctcgtctctgacgctttcta tgttccgca

> SEQ ID NO 32 The DNA sequence of Pt-VirD2-4 in a VirE promoter/VirF terminator cassette where Pt-VirD2 has the wild type NLS1. The Pt-VirD2-4 has the tobacco SSU Transit peptide+5aa, the wt minimal VirD2-T4SS signal.

cggctgctcgtcaccaacaatgctcacagggatacgatggcctcgacagt caggccgcgtgcgtccagtctttccagttcctccctttcaggtcgattgt ggcatcatttattgcctgctcattgcagttgaaacgcgatatccgtttca agacccgagtatggatggtactttggagatatgagcacttgccagacttc ccggtcggagacttaaaaattccgataagaggttactgacgacggacctt ataagtgggtcgtctagtggtcgcactgatcaaaacagttccgcccccgg ctttgctcaaagtagcaaagcagctttatctcgggttgcagaggattttc tggaaaaccgcaattttgcaggagagaacatatggcttcctcagttcttt cctccgcagcagttgccacccgcagcaatgttgctcaagctaacatggtt gcacctttcactggccttaagtcagctgcctcattccctgtttcaaggaa gcaaaaccttgacatcacttccattgccagcaacggcggaagagtgcaat gcatgCAGGTGTGGCCAGCGATGGCTCCCGATCGGGCCCAAGTAATCATT CGCATTGTGCCAGGAGGTGGAACCAAGACCCTTCAGCAGATAATCAATCA GCTGGAGTACCTGTCCCGAAAGGGAAAGCTGGAACTCCAGCGTTCAGCCC GGCATCTCGATATTCCCGTTCCGCCGGATCAAATCCGTGAGCTTGCCCAA AGCTGGGTTACGGAGGCCGGGATTTATGACGAAAGTCAGTCAGACGATGA CAGGCAACAAGACTTAACAACACACATTATTGTGAGCTTCCCCGCAGGTA CCGACCAAACCGCAGCTTATGAAGCAAGCCGGGAATGGGCAGCCGAGATG TTTGGGTCAGGATACGGGGGTGGCCGCTATAACTATCTGACAGCCTACCA CGTGGACCGCGATCATCCACATTTACATGTCGTGGTCAATCGTCGGGAAC TTCTGGGGCAGGGGTGGCTGAAAATATCCAGGCGCCATCCCCAGCTGAAT TATGACGGCTTACGGAAAAAGATGGCAGAGATTTCACTTCGTCACGGCAT AGTCCTGGATGCGACTTCCCGAGCAGAAAGGGGAATAGCAGAGCGACCAA TCACCTATGCTGAATATCGACGTCTTGAGCGGATGCTCGAAGTTATGGCA GAAGTTCGGCCCATAGCCCGATCCATTAAAACGGCTCACGACGATGCGCG AGCGGAATTAATGTCGGCGGACAGACCTCGATCAACGCGCGGTCTATGAT Tctagaatccgtgctgttcgtcacccgcttatagttgccatcacaatcgg atttcaactgccccggtctttaggtgcggccgaggacaatgtagcacgca cttacggtgcgtctcgtctctgacgctttctatgttccgca

> SEQ ID NO 33 DNA sequence introducing a C to T point mutation at nucleotide 473 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring streptomycin resistance

TTAATTAAAATAAGCATCGGCTAACTCTGTGCCAGCAGICGCGGTAATAC AGAGGATGCAAGCGTTATCGGCGCGCC

> SEQ ID NO 34 DNA sequence introducing a C to A point mutation at nucleotide 861 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring streptomycin resistance

TTAATTAACCTGGGGAGTACGTTCGCAAGAATGAAACTAAAAGGAATTGA CGGGGGCCCGCACAAGCGGGGCGCGCC

> SEQ ID NO 35 DNA sequence introducing a C to T point mutation at nucleotide 861 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring streptomycin resistance

TTAATTAACCTGGGGAGTACGTTCGCAAGAATGAAACTIAAAGGAATTGA CGGGGGCCCGCACAAGCGGGGCGCGCC

> SEQ ID NO 36 DNA sequence introducing a G to A point mutation at nucleotide 1013 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring spectinomycin resistance

TTAATTAAGAACGCGGACACAGGTGGTGCATGGCTGTCATCAGCTCGTGC CGTAAGGTGTTGGGTTAAGGGCGCGCC

> SEQ ID NO 37 DNA sequence introducing a A to C point mutation at nucleotide 1139 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring spectinomycin resistance

TTAATTAACCGGTGATAAGCCGGAGGAAGGTGAGGATGCCGTCAAGTCAT CATGCCCCTTATGCCCTGGGGCGCGCC

> SEQ ID NO 38 DNA sequence introducing a C to T point mutation at nucleotide 1140 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring spectinomycin resistance

TTAATTAACGGTGATAAGCCGGAGGAAGGTGAGGATGATGTCAAGTCATC ATGCCCCTTATGCCCTGGGGGCGCGCC

> SEQ ID NO 39 DNA sequence introducing a G to A point mutation at nucleotide 1141 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring spectinomycin resistance

TTAATTAAGGTGATAAGCCGGAGGAAGGTGAGGATGACATCAAGTCATCA TGCCCCTTATGCCCTGGGCGGCGCGCC

> SEQ ID NO 40 DNA sequence introducing a G to A point mutation at nucleotide 1335 in tobacco plastid 16S RNA coding sequence flanked by PacI and AscI conferring spectinomycin resistance

TTAATTAAGTCAGCCATACGGCGGTGAATTCGTTCCCGAGCCTTGTACAC ACCGCCCGTCACACTATGGGGCGCGCC

> SEQ ID NO 41 DNA sequence introducing streptomycin resistance and a silent mutation in the tobacco rps12 coding sequence. Flanked by PacI and AscI restriction sites.. C271T changes Pro to Ser conferring streptomycin resistance. A270G is a silent mutation.

TTAATTAAGTCTTAGTAAGAGGGGGAAGGGTTAAGGATTTGTCCGGTGTG AGATATCACATTGTTCGAGGAAGGCGCGCC

> SEQ ID NO 42 DNA sequence of Pt-ACC2-virD2 in a VirE promoter/VirF terminator cassette (P/T are in lower case) where Pt-ACC2-virD2 has A. thaliana ACC2 transit peptide+6aa fused with wt minimal VirD2 and T4SS signal.

ttatcgatgagtaaaagcgttgttacactatttttatttcacattcgtta taagacaattgcaaatgtagcaagtatattcagtattgactgtaaatgta ctgttgatttcatattgagcagggctagacttccatccgtctacccgggc acatttcgtgctggagtatccagaccttccgctttctttggaggaagcta tggagatgagagctttgggttcttcgtgttctactggtaatggaggttct gctccgattaccctcacgaatatatctccttggatcacaacagtttttcc atctacagtgaagctgagaagtagtttgagaaccttcaaaggagtttcgt caagagtgagaacctttaaaggagtttcttcgacaagagttttgtctcgg accaaacaacagtttcctctgttttgtttcctaaaccctgatccgATGGC TCCCGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCA AGACCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGA AAGCTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCC GGATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTT ATGACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACAC ATTATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGC AAGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCC GCTATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTA CATGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAAT ATCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGG CAGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCA GAAAGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCT TGAGCGGATGCTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCA TTAAAACGGCTCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGA CCTCGATCAACGCGCGGTCTATGAttctagaatccgtgctgttcgtcacc cgcttatagttgccatcacaatcggatttcaactgccccggtctttaggt gcggccgaggacaatgtagcacgcacttacggtgcgtctcgtctctgacg ctttctatgttccgca

> SEQ ID NO 43 DNA sequence of PtMKK4-virD2 in a VirE promoter/VirF terminator cassette (P/T are in lower case) where PtMKK4-virD2 has A. thaliana MKK4 transit peptide fused with wt minimal VirD2 and T4SS signal.

ttatcgatgagtaaaagcgttgttacactatttttatttcacattcgtta taagacaattgcaaatgtagcaagtatattcagtattgactgtaaatgta ctgttgatttcatattgagcagggctagacttccatccgtctacccgggc acatttcgtgctggagtatccagaccttccgctttctttggaggaagctA TGAGACCGATTCAATCGCCTCCAGGAGTTTCCGTTCCGGTGAAAAGCCGT CCCCGTCGCCGTCCTGATCTTACCTTACCGCTTCCTCAACGCGATGTTTC TCTCGCTGTACCTCTTCCTCTCCCACCTACTTCCGGTGGTTCCGGTGGCT CTAGTGGATCTGCGCCGTCTTCTGGTGGTTCGGCGTCTTCAACGATGGCT CCCGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCAA GACCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGAA AGCTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCCG GATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTTA TGACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACACA TTATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGCA AGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCCG CTATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTAC ATGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAATA TCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGGC AGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCAG AAAGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCTT GAGCGGATGCTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCAT TAAAACGGCTCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGAC CTCGATCAACGCGCGGTCTATGAttctagaatccgtgctgttcgtcaccc gcttatagttgccatcacaatcggatttcaactgccccggtctttaggtg cggccgaggacaatgtagcacgcacttacggtgcgtctcgtctctgacgc tttctatgttccgca

> SEQ ID NO 44 DNA sequence of pEX18Kan. pEX18Tc (Accession no. AF047519) derivative with kanamycin resistance as selectable marker.

tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccg gagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccg tcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatg cggcatcagagcagattgtactgagagtgcaccataatcggcattttctt ttgcgtttttatttgttaactgttaattgtccttgttcaaggatgctgtc tttgacaacagatgttttcttgcctttgatgttcagcaggaagctaggcg caaacgttgattgtttgtctgcgtagaatcctctgtttgtcatatagctt gtaatcacgacattgtttcctttcgcttgaggtacagcgaagtgtgagta agtaaaggttacatcgttaggatcaagatccatttttaacacaaggccag ttttgttcagcggcttgtatgggccagttaaagaattagaaacataacca agcatgtaaatatcgttagacgtaatgccgtcaatcgtcatttttgatcc gcgggagtcagtgaacagataccatttgccgttcattttaaagacgttcg cgcgttcaatttcatctgttactgtgttagatgcaatcagcggtttcatc acttttttcagtgtgtaatcatcgtttagctcaatcataccgagagcgcc gtttgctaactcagccgtgcgttttttatcgctttgcagaagtttttgac tttcttgacggaagaatgatgtgcttttgccatagtatgctttgttaaat aaagattcttcgccttggtagccatcttcagttccagtgtttgcttcaaa tactaagtatttgtggcctttatcttctacgtagtgaggatctctcagcg tatggttgtcgcctgagctgtagttgccttcatcgatgaactgctgtaca ttttgatacgtttttccgtcaccgtcaaagattgatttataatcctctac accgttgatgttcaaagagctgtctgatgctgatacgttaacttgtgcag ttgtcagtgtttgtttgccgtaatgtttaccggagaaatcagtgtagaat aaacggatttttccgtcagatgtaaatgtggctgaacctgaccattcttg tgtttggtcttttaggatagaatcatttgcatcgaatttgtcgctgtctt taaagacgcggccagcgtttttccagctgtcaatagaagtttcgccgact ttttgatagaacatgtaaatcgatgtgtcatccgcatttttaggatctcc ggctaatgcaaagacgatgtggtagccgtgatagtttgcgacagtgccgt cagcgttttgtaatggccagctgtcccaaacgtccaggccttttgcagaa gagatatttttaattgtggacgaatcgaactcaggaacttgatatttttc atttttttgctgttcagggatttgcagcatatcatggcgtgtaatatggg aaatgccgtatgtttccttatatggcttttggttcgtttctttcgcaaac gcttgagttgcgcctcctgccagcagtgcggtagtaaaggttaatactgt tgcttgttttgcaaactttttgatgttcatcgttcatgtctcctttttta tgtactgtgttagcggtctgcttcttccagccctcctgtttgaagatggc aagttagttacgcacaataaaaaaagacctaaaatatgtaaggggtgacg ccaaagtatacactttgccctttacacattttaggtcttgcctgctttat cagtaacaaacccgcgcgatttacttttcgacctcattctattagactct cgtttggattgcaactggtctattttcctcttttgtttgatagaaaatca taaaaggatttgcagactacgggcctaaagaactaaaaaatctatctgtt tcttttcattctctgtattttttatagtttctgttgcatgggcataaagt tgcctttttaatcacaattcagaaaatatcataatatctcatttcactaa ataatagtgaacggcaggtatatgtgatgggttaaaaaggatcgatcctc tagctagagtcgatcttcgccagcagggcgaggatcgtggcatcaccgaa ccgcgccgtgcgcgggtcgtcggtgagccagagtttcagcaggccgccca ggcggcccaggtcgccattgatgcgggccagctcgcggacgtgctcatag tccacgacgcccgtgattttgtagccctggccgacggccagcaggtaggc cgacaggctcatgccggccgccgccgccttttcctcaatcgctcttcgtt cgtctggaaggcagtacaccttgataggtgggctgcccttcctggttggc ttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagc cggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaat aagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacct atcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaac cctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaa aaatcgctataatgaccccgaagcagggttatgcagcggaaaagcgctgc ttccctgctgttttgtggaatatctaccgactggaaacaggcaaatgcag gaaattactgaactgaggggacaggcgagagacgatgccaaagagctaca ccgacgagctggccgagtgggttgaatcccgcgcggccaagaagcgccgg cgtgatgaggctgcggttgcgttcctggcggtgagggcggatgtcgatat gcgtaaggagaaaataccgcatcaggcgcatatttgaatgtatttagaaa aataaacaaaaagagtttgtagaaacgcaaaaaggccatccgtcaggatg gccttctgcttaatttgatgcctggcagtttatggcgggcgtcctgcccg ccaccctccgggccgttgcttcgcaacgttcaaatccgctcccggcggat ttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaag gcccagtctttcgactgagcctttcgttttatttgatgcctggcagttcc ctactctcgcatggggagaccccacactaccatcggcgctacggcgtttc acttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgcc aggcaaattctgttttatcagaccgcttctgcgttctgatttaatctgta tcaggctgaaaatcttctctcatccgccaaaacagccaagctcgccattc gccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctctt cgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagt tgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccag tgccaagcttgcatgcctgcaggtcgactctagaggatccccgggtaccg agctcgaattcgtaatcatggtcatagctgtttcctgtgtgaaattgtta tccgctcacaattccacacaacatacgagccggaagcataaagtgtaaag cctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctca ctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaat cggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgctt cctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggta tcagctcactcaaaggcggtaatacggttatccacagaatcaggggataa cgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgta aaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgag catcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggact ataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctg ttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggga agcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgta ggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaaga cacgacttatcgccactggcagcagccactggtaacaggattagcagagc gaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacg gctacactagaaggacagtatttggtatctgcgctctgctgaagccagtt accttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgc tggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaa aaggatctcaagaagatcctttgatcttttctacggggtctgacgctcag tggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaag gatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatct aaagtatatatgagtaaacttggtctgacaatcgatgcaagggttggttt gTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGG CAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA TTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACG GGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCAT GAGCTTGCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCCAGTGTTACAA CCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACT GCAATTTATTCACATCAGGATTATCAATACCATATTTTTGAAAAAGCCGT TTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAG ATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTA TTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGA GTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTgTGCATTTCTTTCCA GACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCAT CAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCAAGACGAAATACG CGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCG CAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATT CTTCTAATACCTGGAATGCTGTTTTTCCGGGGATCGCAGTGGTGAGTAAC CAcGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCAT AAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGG CAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTC CCATACAAGCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGTGC CCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCC TCGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTT ATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGC AATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTCCCCCCCCCCCC CATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCAGGctaagaaa ccattattatcatgacattaacctataaaaataggcgtatcacgaggccc tttcgtc

> SEQ ID NO 45. EcoRI-HindIII fragment of Pt-virD2 flanked with 500 bp of virD1 and 500 bp of virD3. The PtVirD2 has the tobacco SSU Transit peptide, the wt minimal VirD2-T4SS signal listed as SEQ ID NO 31.

gaattcttccgctttctttggaggaagctatgtcaaaacacaccagagcc acgtcgagtgagactaccatcaaccagcatcgatccctgaaagttgaagg gttcaaggtcgtgagtgcccgtctgcgatcggccgagtatgaaacctttt cctatcaagcgcgcctgctgggactttcggatagtatggcaattcgcgtt gcggtgcgccgcatcgggggctttctcgaaatagatgcagacacacgaga aaagatggaagccatacttcagtccatcggaatactctcaagcaatgtat ccatgcttctatctgcctacgccgaagaccctcgatcggatctggaggct gtgcgagatgaacgtattgcttttggcgaggctttcgccgccctcgatgg actactgcgctccattttgtccgtatcccggcgacggatcgacggtcgct cgttactgaaaggtgccttgtagcacttgaccacgcacctgacgggagaa aattggATGGCTTCCTCAGTTCTTTCCTCCGCAGCAGTTGCCACCCGCAG CAATGTTGCTCAAGCTAACATGGTTGCACCTTTCACTGGCCTTAAGTCAG CTGCCTCATTCCCTGTTTCAAGGAAGCAAAACCTTGACATCACTTCCATT GCCAGCAACGGCGGAAGAGTGCAATGCATGCAGGTGTGGCCAGCGATGGC TCCCGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTGGAACCA AGACCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGAAAGGGA AAGCTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGTTCCGCC GGATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCGGGATTT ATGACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAACAACACAC ATTATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTATGAAGC AAGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGGGTGGCC GCTATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCCACATTTA CATGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCTGAAAAT ATCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAAAGATGG CAGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCCCGAGCA GAAAGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATCGACGTCT TGAGCGGATGCTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCCGATCCA TTAAAACGGCTCACGACGATGCGCGAGCGGAATTAATGTCGGCGGACAGA CCTCGATCAACGCGCGGTCTATGAagacaggaaggaccgaataatggcaa atggtcagttcacgatacgctctgctcgcccggcctccgtcggactgaca ggcgaacggcgtggagccgcatccgcctctagctctgcactgtccaatgt tcaaagagatgttagggataggctgattccaactagctcaccaagattac caaatgcagccatattgcgtgattcctcgggaagagcgtcgactggtctg cggtacatggcggctactcttcattggtctgcgatcgcgccattatcgct aataaacagcaacgacctggctccggccgcttatgactttgagacgcgaa ataacgcaagaaatgtgactgccaaagtcggcagggcagtccctgttccc aagcaaggcgggctcggcaaaacgctcgcacccgtaccccttagtacacg tatatcaagggtcaattccgaccgaagactgcccgctgacgcagaagacc gccctgaaacgcgcgacccccagaaagctt

> SEQ ID NO 46. EcoRI-HindIII fragment of PtACC2-virD2 flanked with 500 bp of virD1 and 500 bp of virD3. The PtACC2-VirD2 has the Arabidopsis ACC2 Transit peptide, the wt minimal VirD2-T4SS signal listed as SEQ ID NO 42.

gaattcttccgctttctttggaggaagctatgtcaaaacacaccagagcc acgtcgagtgagactaccatcaaccagcatcgatccctgaaagttgaagg gttcaaggtcgtgagtgcccgtctgcgatcggccgagtatgaaacctttt cctatcaagcgcgcctgctgggactttcggatagtatggcaattcgcgtt gcggtgcgccgcatcgggggctttctcgaaatagatgcagacacacgaga aaagatggaagccatacttcagtccatcggaatactctcaagcaatgtat ccatgcttctatctgcctacgccgaagaccctcgatcggatctggaggct gtgcgagatgaacgtattgcttttggcgaggctttcgccgccctcgatgg actactgcgctccattttgtccgtatcccggcgacggatcgacggtcgct cgttactgaaaggtgccttgtagcacttgaccacgcacctgacgggagaa aattggATGGAGATGAGAGCTTTGGGTTCTTCGTGTTCTACTGGTAATGG AGGTTCTGCTCCGATTACCCTCACGAATATATCTCCTTGGATCACAACAG TTTTTCCATCTACAGTGAAGCTGAGAAGTAGTTTGAGAACCTTCAAAGGA GTTTCGTCAAGAGTGAGAACCTTTAAAGGAGTTTCTTCGACAAGAGTTTT GTCTCGGACCAAACAACAGTTTCCTCTGTTTTGTTTCCTAAACCCTGATC CGATGGCTCCCGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGT GGAACCAAGACCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCG AAAGGGAAAGCTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCG TTCCGCCGGATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCC GGGATTTATGACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAAC AACACACATTATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTT ATGAAGCAAGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGG GGTGGCCGCTATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCC ACATTTACATGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGC TGAAAATATCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAA AAGATGGCAGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTC CCGAGCAGAAAGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATC GACGTCTTGAGCGGATGCTCGAAGTTATGGCAGAAGTTCGGCCCATAGCC CGATCCATTAAAACGGCTCACGACGATGCGCGAGCGGAATTAATGTCGGC GGACAGACCTCGATCAACGCGCGGTCTATGAagacaggaaggaccgaata atggcaaatggtcagttcacgatacgctctgctcgcccggcctccgtcgg actgacaggcgaacggcgtggagccgcatccgcctctagctctgcactgt ccaatgttcaaagagatgttagggataggctgattccaactagctcacca agattaccaaatgcagccatattgcgtgattcctcgggaagagcgtcgac tggtctgcggtacatggcggctactcttcattggtctgcgatcgcgccat tatcgctaataaacagcaacgacctggctccggccgcttatgactttgag acgcgaaataacgcaagaaatgtgactgccaaagtcggcagggcagtccc tgttcccaagcaaggcgggctcggcaaaacgctcgcacccgtacccctta gtacacgtatatcaagggtcaattccgaccgaagactgcccgctgacgca gaagaccgccctgaaacgcgcgacccccagaaagctt

> SEQ ID NO 47. EcoRI-HindIII fragment of PtMKK4-virD2 flanked with 500 bp of virD1 and 500 bp of virD3. The PtMKK4-VirD2 has the Arabidopsis MKK4 Transit peptide, the wt minimal VirD2-T4SS signal listed as SEQ ID NO 43.

gaattcttccgctttctttggaggaagctatgtcaaaacacaccagagcc acgtcgagtgagactaccatcaaccagcatcgatccctgaaagttgaagg gttcaaggtcgtgagtgcccgtctgcgatcggccgagtatgaaacctttt cctatcaagcgcgcctgctgggactttcggatagtatggcaattcgcgtt gcggtgcgccgcatcgggggctttctcgaaatagatgcagacacacgaga aaagatggaagccatacttcagtccatcggaatactctcaagcaatgtat ccatgcttctatctgcctacgccgaagaccctcgatcggatctggaggct gtgcgagatgaacgtattgcttttggcgaggctttcgccgccctcgatgg actactgcgctccattttgtccgtatcccggcgacggatcgacggtcgct cgttactgaaaggtgccttgtagcacttgaccacgcacctgacgggagaa aattggATGAGACCGATTCAATCGCCTCCAGGAGTTTCCGTTCCGGTGAA AAGCCGTCCCCGTCGCCGTCCTGATCTTACCTTACCGCTTCCTCAACGCG ATGTTTCTCTCGCTGTACCTCTTCCTCTCCCACCTACTTCCGGTGGTTCC GGTGGCTCTAGTGGATCTGCGCCGTCTTCTGGTGGTTCGGCGTCTTCAAC GATGGCTCCCGATCGGGCCCAAGTAATCATTCGCATTGTGCCAGGAGGTG GAACCAAGACCCTTCAGCAGATAATCAATCAGCTGGAGTACCTGTCCCGA AAGGGAAAGCTGGAACTCCAGCGTTCAGCCCGGCATCTCGATATTCCCGT TCCGCCGGATCAAATCCGTGAGCTTGCCCAAAGCTGGGTTACGGAGGCCG GGATTTATGACGAAAGTCAGTCAGACGATGACAGGCAACAAGACTTAACA ACACACATTATTGTGAGCTTCCCCGCAGGTACCGACCAAACCGCAGCTTA TGAAGCAAGCCGGGAATGGGCAGCCGAGATGTTTGGGTCAGGATACGGGG GTGGCCGCTATAACTATCTGACAGCCTACCACGTGGACCGCGATCATCCA CATTTACATGTCGTGGTCAATCGTCGGGAACTTCTGGGGCAGGGGTGGCT GAAAATATCCAGGCGCCATCCCCAGCTGAATTATGACGGCTTACGGAAAA AGATGGCAGAGATTTCACTTCGTCACGGCATAGTCCTGGATGCGACTTCC CGAGCAGAAAGGGGAATAGCAGAGCGACCAATCACCTATGCTGAATATCG ACGTCTTGAGCGGATGCTCGAAGTTATGGCAGAAGTTCGGCCCATAGCCC GATCCATTAAAACGGCTCACGACGATGCGCGAGCGGAATTAATGTCGGCG GACAGACCTCGATCAACGCGCGGTCTATGAagacaggaaggaccgaataa tggcaaatggtcagttcacgatacgctctgctcgcccggcctccgtcgga ctgacaggcgaacggcgtggagccgcatccgcctctagctctgcactgtc caatgttcaaagagatgttagggataggctgattccaactagctcaccaa gattaccaaatgcagccatattgcgtgattcctcgggaagagcgtcgact ggtctgcggtacatggcggctactcttcattggtctgcgatcgcgccatt atcgctaataaacagcaacgacctggctccggccgcttatgactttgaga cgcgaaataacgcaagaaatgtgactgccaaagtcggcagggcagtccct gttcccaagcaaggcgggctcggcaaaacgctcgcacccgtaccccttag tacacgtatatcaagggtcaattccgaccgaagactgcccgctgacgcag aagaccgccctgaaacgcgcgacccccagaaagctt

>SEQ ID NO 48 - AM102 Primer

GTTGAGTCGATACTTCGGCG

>SEQ ID NO 49 - AM103

GGCACCAGTCCTACATTTTG

>SEQ ID NO 50 - GFP11

RDHMBLHEYBNAAGIT

>SEQ ID NO 51 - Left Border sequence

TGGCAGGATATATTGTGGTGTAAAC

>SEQ ID NO 52 - Spacer between Left Border repeats

CGAGCTGTATAGGCTGGCTGG

>SEQ ID NO 53 - Right Border sequence

TGACAGGATATATTGGCGGGTAAAC

>SEQ ID NO 54 - Overdrive sequence

TAAGTCGCTGTATGTGTTTGTTTG

SEQ ID NO 55 DNA sequence introducing streptomycin resistance and a silent mutation in the tobacco rps12 coding sequence. Flanked by PacI and AscI restriction sites.. C271T changes Pro to Ser conferring streptomycin resistance. T276A is a silent mutation.

TTAATTAAGTCTTAGTAAGAGGGGGAAGGGTTAAGGATTTATCCGGAGTG AGATATCACATTGTTCGAGGAAGGCGCGCC

>SEQ ID NO 56. A variant of SEQ ID NO 29 (FIG. 11 ), with shorter plastid targeting sequences. Note that the PacI and AscI sites have been blunted.

CCGCCAGCGTTCATCCTGAGCCAGGATCGAACTCTCCATGAGATTCATAG TTGCATTACTTATAGCTTCCTTGTTCGTAGACAAAGCGGATTCGGAATTG TCTTTCATTCCAAGGCATAACTTGTATCCATGCGCTTCATATTCGCCCGG AGTTCGCTCCCAGAAATATAGCCATCCCTGCCCCCTCACGTCAATCCCAC GAGCCTCTTATCCATTCTCATTGAACGACGGCGGGGGAGCAAATCCAACT AGAAAAACTCACATTGGGCTTAGGGATAATCAGGCTCGAACTGATGACTT CCACCACGTCAAGGTGACACTCTACCGCTGAGTTATATCCCTTCCCCGCC CCATCGAGAAATAGAACTGACTAATCCTAAGTCAAAGGGTCGAGAAACTC AACGCCACTATTCTTGAACAACTTGGAGCCGGGCCTTCTTTTCGCACTAT TACGGATATGAAAATAATGGTCAAAATCGGATTCAATTCCCgggTTGGGT TGCGCTATATATATGAAAGAGTATACAATAATGATGTATTTGagttgtag ggagggatttATGgGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTAT CAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCC GTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGA TATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGC GAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGC GAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCAT TCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGC GCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGAT CTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGG TCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTG AGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCT GGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGC AGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGG AGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCT TATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGA AGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTgGGCAAAg aacaaaaactcatttctgaagaagacttgtgaGATCCTGGCCTAGTCTAT AGGAGGTTTTGAAAAGAAAGGAGCAATAATCATTTTCTTGTTCTATCAAG AGGGTGCTATTGCTCCTTTCTTTTTTTCTTTTTATTTATTTACTAGTATT TTACTTACATAGACTTTTTTGTTTACATTATAGAAAAAGAAGGAGAGGTT ATTTTCTTGCATTTATTCATGcccGGGCAACTGCCCCTATCGGAAATAGG ATTGACTACCGATTCCGAAGGAACTGGAGTTACATCTCTTTTCCATTCAA GAGTTCTTATGCGTTTCCACGCCCCTTTGAGACCCCGAAAAATGGACAAA TTCCTTTTCTTAGGAACACATACAAGATTCGTCACTACAAAAAGGATAAT GGTAACCCTACCATTAACTACTTCATTTATGAATTTCATAGTAATAGAAA TACATGTCCTACCGAGACAGAATTTGGAACTTGCTATCCTCTTGCCTAGC AGGCAAAGATTTACCTCCGTGGAAAGGATGATTCATTCGGATCGACATGA GAGTCCAACTACATTGCCAGAATCCATGTTGTATATTTGAAAGAGGTTGA CCTCCTTGCTTCTCTCATGGTACACTCCTCTTCCCGCCGAGCCCCTTTTC TCCTCGGTCCACAGAGACAAAATGTAGGACTGGTGCCAACAATTCATCAG ACTCACTAAGTCGGGATCACTAACTAATACTAATCTAATATAATAGTCTA ATATATCTAATATAATAGAAAATACTAATATAATAGAAAAGAACTGTCTT TTCTGTATACTTTCCCCGGTTCCGTTGCTACCGCGGGCTTTACGCAATCG ATCGGATTAGATAGAT

>SEQ ID NO 57. attB sequence.

CCAGGGCGTGCCCTTGGGCTCCCCGGGCG

>SEQ ID NO 58. attP sequence.

ACTGGGGTAACCTTTGAGTTCTCTCAGTT

>SEQ ID NO 59. attL sequence.

CCAGGGCGTGCCCTTGAGTTCTCTCAGTT

>SEQ ID NO 60. attR sequence.

ACTGGGGTAACCTTTGGGCTCCCCGGGCG

>SEQ ID NO 61 Oligonucleotide confers streptomycin resistance and the silent mutations create a KpnI restriction site in the rps12 plastid gene.

GTCTTAGTAAGAGGGGGAAGGGTTAAGGATTTATCCGGTGTGAGGTACCA CATTGTTCGAGGAACCCTAGATGCTGTC

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While certain features of the invention have been described herein, many modifications, substitutions, changes, and equivalents will now occur to those of ordinary skill in the art. It is, therefore, to be understood that the appended claims are intended to cover all such modifications and changes as fall within the true spirit of the invention. 

What is claimed is:
 1. A method for delivering a polynucleotide of interest to plant cell chloroplasts, said method comprising a) providing an Agrobacterium variant strain lacking VirD2 in the Vir operon, b) introducing a binary vector plasmid encoding a polynucleotide fusion construct encoding a VirD2 variant protein with a chloroplast transit peptide operably linked to a promoter and terminator which drives expression in said Agrobacterium variant strain and a T DNA comprising a chloroplast sequence mediating homologous recombination into the chloroplast genome, a nucleic acid encoding a polypeptide of interest, and a nucleic acid encoding a selectable marker, wherein said VirD2 variant protein forms a complex with said T-DNA and delivers said complex to the chloroplast, c) selecting infected plant cells which survive in the presence of a selection agent, thereby identifying cells expressing said polynucleotide of interest.
 2. The method of claim 1, wherein said VirD2 variant protein is expressed from a binary plasmid comprising a sequence selected from SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 42 and SEQ ID NO:
 43. 3. The method of claim 1, wherein the selectable marker in the binary vector is encoded by a sequence selected from SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO 40, SEQ ID NO: 41 and SEQ ID NO:
 55. 4. The method of claim 1, wherein said polynucleotide fusion construct is present in a binary vector backbone selected from a pAGa vector of SEQ ID NO: 22, a pAGb vector of SEQ ID NO: 23, a pAGc vector of SEQ ID NO: 24, and a pAGd vector of SEQ ID NO:
 25. 5. The method of claim 4, wherein said pAG binary vector encoding any PtVirD2 variant is introduced into a XYA105ΔvirD2 strain for co-cultivation and chloroplast transformation in a plant and said ptVirD2 variant is encoded by a sequence selected from SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 42 and SEQ ID NO:
 43. 6. The method of claim 1, wherein said binary vector of , wherein said binary is a pAG vector comprising a PtVirD2 variant encoded by SEQ ID NO:
 29. 7. An isolated Agrobacterium strain XYA105Pt variant selected from XYA105Pt45, XYA105Pt46, and XYA105Pt47, comprising a reconstituted virD operon encoding a VirD2 variant selected from SEQ ID NO: 45, SEQ ID NO: 46 and SEQ ID NO: 47, said variant Agrobacterium strain optionally comprising a binary vector harboring a T-DNA encoding a nucleic acid of interest and a selectable marker, said T-DNA comprising a sequence which mediates homologous recombination in a chloroplast genome in said plant cell.
 8. The method of claim 1, wherein said plant is infected with a XYA105ΔvirD2 Agrobacterium strain carrying said binary vector comprising said nucleic acids encoding said T-DNA and said Pt-VirD2 variant.
 9. A method for delivering a polynucleotide of interest to plant cell chloroplasts, said method comprising a) infiltrating a plant with XYA105Pt variant Agrobacterium strain as claimed in claim 7, said strain harboring said T-DNA, b) culturing treated leaf sections in selection media comprising a selective agent; and c) isolating transplastomic plant cells resistant to said selection agent.
 10. The method of claim 9, wherein saidVirD2 variant is encoded by SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 42 and SEQ ID NO:
 43. 11. The method of claim 1, when the VirD2 variant is incorporated in the virD operon and has a sequence selected from SEQ ID NO: 45, SEQ ID NO: 46 or SEQ ID NO: 47 for reconstitution of said operon.
 12. The method of claim 1 wherein said plant cell is a tobacco, Arabidopsis, corn, soybean, sorghum, wheat, rice, alfalfa, sunflower, or Brassica sp. cell.
 13. The method of claim 1 wherein selectable marker is a protein.
 14. The method of claim 13, wherein said selectable marker protein is a visible or chemical selectable marker protein.
 15. The method of claim 14, wherein said selectable marker confers spectinomycin resistance, streptomycin resistance.
 16. The method of claim 14, wherein said selectable marker is Green Fluorescent Protein, m-cherry, m-scarlett, luciferase or GUS encoding the beta-glucuronidase enzyme.
 17. The method of claim 14, wherein said visual-selectable marker is selected from aadA^(au,) encoded by the sequence provided in GENBANK ID NO. HQ023426 and bar^(au) encoded by the sequence provided in GENBANK ID NO. GH980699.
 18. The method of claim 1, wherein said plant cell is a monocot.
 19. The method of claim 1, wherein said plant cell is a dicot.
 20. The method of claim 1, wherein said plant cell is the plant cell is a Brassica sp., tobacco, Arabidopsis, corn, soybean, sorghum, wheat, rice, alfalfa, or sunflower cell.
 21. The method of claim 1, further comprising growing the plant cell to produce a plant.
 22. The method of claim 1, further comprising exposing a flower from said plant to said Agrobacterium, harvesting seed from said flower and optionally germinating said seed on selective media.
 23. The method of claims 1, further comprising exposing a flower from said plant to said Agrobacterium, harvesting seed from said flower and identifying transplastomic events by leaf phenotypic change.
 24. A binary vector for use in the method of claim 5 comprising a transgene expressing a protein or RNA of interest.
 25. The vector of claim 24, present in a transplastomic plant.
 26. A method for delivering a polynucleotide of interest to plant cell chloroplasts, said method comprising a) provide an Agrobacterium variant strain with a wild type Vir operon, b) introducing a binary vector plasmid encoding a polynucleotide fusion construct encoding a VirD2 variant protein operably linked to a promoter and terminator which drives expression in said Agrobacterium variant strain, and a T DNA comprising a chloroplast sequence mediating homologous recombination into the chloroplast genome, a nucleic acid encoding a polypeptide of interest, and a nucleic acid encoding a selectable marker, wherein said VirD2 variant protein forms a complex with said T-DNA and delivers said complex to the chloroplast, c) selecting infected plant cells which survive in the presence of a selection agent, thereby identifying cells expressing said polynucleotide of interest.
 27. The method of claim 26, wherein said VirD2 variant protein is expressed from a binary plasmid comprising a sequence selected from SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 42 and SEQ ID NO:
 43. 28. The method of claim 8, wherein said plant cell is the plant cell is a Brassica sp., tobacco, Arabidopsis, corn, soybean, sorghum, wheat, rice, alfalfa, or sunflower cell.
 29. The method of claim 8, further comprising growing the plant cell to produce a plant.
 30. The method of claim 8, further comprising exposing a flower from said plant to said Agrobacterium, harvesting seed from said flower and optionally germinating said seed on selective media.
 31. The method of claim 9, wherein said plant cell is the plant cell is a Brassica sp., tobacco, Arabidopsis, corn, soybean, sorghum, wheat, rice, alfalfa, or sunflower cell.
 32. The method of claim 9, further comprising growing the plant cell to produce a plant.
 33. The method of claim 9, further comprising exposing a flower from said plant to said Agrobacterium, harvesting seed from said flower and optionally germinating said seed on selective media.
 34. A kit for practicing the method of claim
 1. 